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[176857_s_at]

Description ZK616.B /REP_DB=WormBase Gene ID /WP=CE26344 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60943.1
Cluster 6grp_total > 84      4grp_total > 48      6grp_cgc > 65      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 384
1 2 3 4 range
A 15
(-3)
A 		293.4
(1.4)
P 		398.1
(3)
P 		87.1
(-1.9)
A 		383
B 14.5
(-3)
A 		277.9
(3)
P 		209.7
(1.4)
P 		65.2
(-1.8)
A 		263
C 44.8
(-3)
A 		279.6
(1.6)
P 		354.3
(3)
P 		132.6
(-1.3)
P 		310
avr 24.8
(-3) 		283.6
(2.2) 		320.7
(3) 		95
(-1.6) 		296

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 196
1 2 3 4 5 6 range
A 75.9
(-3)
A 		172.3
(0.1)
P 		128.9
(-1.3)
P 		111.4
(-1.8)
P 		252.2
(2.7)
M 		260.6
(3)
P 		185
B 71.8
(-3)
A 		152.2
(-0.5)
P 		178.5
(0.3)
A 		255.3
(2.6)
P 		268.1
(3)
P 		169.2
(-0)
P 		196
C 82
(-3)
A 		178.9
(3)
P 		163.5
(2)
A 		169
(2.4)
P 		124.1
(-0.4)
M 		155.5
(1.6)
P 		97
avr 76.6
(-3) 		167.8
(1) 		157
(0.5) 		178.6
(1.4) 		214.8
(3) 		195.1
(2.1) 		138
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 119
1 2 3 4 range
A 5.5
(-3)
A 		63.7
(0.5)
P 		104.1
(3)
P 		12.7
(-2.6)
A 		99
B 4.6
(-3)
A 		68.6
(3)
P 		48.6
(1.1)
P 		13.9
(-2.1)
A 		64
C 14.8
(-3)
A 		80.4
(0.6)
P 		123.5
(3)
P 		22.4
(-2.6)
P 		109
avr 8.3
(-3) 		70.9
(1.5) 		92.1
(3) 		16.3
(-2.4) 		84

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 95
1 2 3 4 5 6 range
A 14
(-3)
A 		61.3
(1.6)
P 		28.1
(-1.6)
P 		28.2
(-1.6)
P 		76.1
(3)
M 		63.5
(1.8)
P 		62
B 25
(-3)
A 		50.7
(-1.2)
P 		43.4
(-1.7)
A 		67.6
(0)
P 		108.9
(3)
P 		63.1
(-0.3)
P 		84
C 30.4
(-3)
A 		54.6
(3)
P 		41.6
(-0.2)
A 		34
(-2.1)
P 		40.2
(-0.6)
M 		52.1
(2.4)
P 		24
avr 23.1
(-3) 		55.5
(0.7) 		37.7
(-1.3) 		43.3
(-0.7) 		75.1
(3) 		59.6
(1.2) 		52
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176857_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19303
Sequence Type Exemplar sequence
Representative Public ID ZK616.B NCBI
Target Description ZK616.B /REP_DB=WormBase Gene ID /WP=CE26344 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60943.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068407(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068407 NCBI Caenorhabditis elegans SaPosin-like Protein family member (spp-7) (spp-7) mRNA, complete cds. 11/11 None
SNAP00000016321 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5412878:5415127:-1 11/11 None
GENEFINDER00000016324 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5412878:5415749:-1 11/11 None
ZK616.9 ENSEMBL cdna:known chromosome:CEL140:IV:5412874:5415749:-1 gene:ZK616.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5412883-5415755(-) 90.49 100.0
Public Domain and Genome References
Gene Title
Gene Symbol spp-7
Ensembl ZK616.9
Entrez Gene 177326 Entrez gene
SwissProt Q9N4M7 EMBL-EBI
WormBase CE26344 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5576 extracellular region inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5179 hormone activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF60943 Saposin-like protein family protein 7 [Caenorhabditis elegans] ref|NP_500808.1| SaPosin-like Protein family member (spp-7) [Caenorhabditis elegans] 1.0E-151
blast CAE72709 Hypothetical protein CBG19946 [Caenorhabditis briggsae] 1.0E-132
blast AAF60943 Saposin-like protein family protein 7 [Caenorhabditis elegans] ref|NP_500808.1| SaPosin-like Protein family member (spp-7) [Caenorhabditis elegans] 1.0E-127
blast CAE72709 Hypothetical protein CBG19946 [Caenorhabditis briggsae] 1.0E-113
Protein Domains
Database ID Description E-Value
Pfam IPR008138 EMBL-EBI Saposin-like type B, 2 8.6E-9
Pfam IPR008138 EMBL-EBI Saposin-like type B, 2 8.6E-9
Sequence
Target Sequence 
>C. ELEGANS:176857_S_AT
agaaatccatttgcgatcggcggtccacttccatcaatcgaagctacaactctgtaccca
gaagaagaagctgaggaaattgaacttccgactactacaacaagaagacgtactacaaca
actacaacaactacaactccaccaccaactactactactacagtacgaactacaacagct
ttgactcgaccaaatccaagtattgagcaagtgagaagagaagagcataatgatagaatt
ggacataagaatgtgttggagataattattccaccaccgcttcgttcaaaatacatgtct
cggaatacaattgtcgaatcttcaagagaaaaagttcccgaaaaagaagaaattgacgag
aaacgtctaacatgtgcgttttgtgagagaatgctggaaaatgccaagaattatgcggtc
acttcaaaaactgacataacctcatttgccaatacagcttgtgcttctcttgccaaagga
acaacatctgatcaatgttatcagatggctgataagaaaatcgcagaactcgcgaaattt
gtggatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAAATCCATTTGCGATCGGCGGTC 23 71 376 Antisense
TCGAAGCTACAACTCTGTACCCAGA 93 601 413 Antisense
ATTGAACTTCCGACTACTACAACAA 603 3 454 Antisense
GAACTACAACAGCTTTGACTCGACC 241 349 542 Antisense
GGAGATAATTATTCCACCACCGCTT 20 519 633 Antisense
CACCACCGCTTCGTTCAAAATACAT 496 201 647 Antisense
ATGCCAAGAATTATGCGGTCACTTC 94 41 776 Antisense
GACATAACCTCATTTGCCAATACAG 401 369 808 Antisense
GCCAATACAGCTTGTGCTTCTCTTG 455 277 823 Antisense
GTGCTTCTCTTGCCAAAGGAACAAC 9 479 836 Antisense
GCAGAACTCGCGAAATTTGTGGATC 590 311 898 Antisense
Sequence Source Affymetrix Proprietary Database