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[176856_at]

Description T27F7.3 /REP_DB=WormBase Gene ID /WP=CE07559 /CHR=2 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG01561.1
Cluster 6grp_highest_10_10 > 70      6grp_high1_10_10 > 17      4grp_highest_8_8 > 63      4grp_high_8_8 > 27      6grp_highest_nonorm > 80      6grp_total > 18      4grp_total > 37      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 200
1 2 3 4 range
A 333.3
(0.7)
P 		282.8
(-1.7)
P 		255.8
(-3)
P 		380.6
(3)
P 		125
B 331.3
(3)
P 		298.8
(1.7)
P 		181
(-3)
P 		259
(0.1)
P 		150
C 313.8
(2.6)
P 		318.9
(3)
P 		249.2
(-3)
P 		279.4
(-0.4)
P 		70
avr 326.1
(3) 		300.2
(1.4) 		228.7
(-3) 		306.3
(1.8) 		97

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 256
1 2 3 4 5 6 range
A 396
(3)
P 		243.3
(-1.4)
P 		193.2
(-2.8)
P 		203.1
(-2.5)
P 		200.9
(-2.6)
P 		185.7
(-3)
P 		210
B 209.7
(3)
P 		161.7
(-2)
P 		152
(-3)
P 		198.3
(1.8)
P 		184.7
(0.4)
P 		189.4
(0.9)
P 		58
C 230
(3)
P 		192.7
(0.5)
P 		221.5
(2.4)
P 		151.5
(-2.3)
P 		147
(-2.6)
P 		140.3
(-3)
P 		90
avr 278.6
(3) 		199.2
(-1.5) 		188.9
(-2) 		184.3
(-2.3) 		177.5
(-2.7) 		171.8
(-3) 		107
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 80
1 2 3 4 range
A 121.4
(3)
P 		61.4
(-2.5)
P 		66.9
(-2)
P 		55.7
(-3)
P 		66
B 105.7
(3)
P 		73.8
(-0)
P 		41.9
(-3)
P 		55.2
(-1.7)
P 		64
C 103.9
(3)
P 		91.7
(1.7)
P 		86.9
(1.2)
P 		47.1
(-3)
P 		57
avr 110.3
(3) 		75.6
(-0.6) 		65.2
(-1.7) 		52.7
(-3) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 56
1 2 3 4 5 6 range
A 73
(1.2)
P 		86.5
(3)
P 		42.1
(-3)
P 		51.5
(-1.7)
P 		60.6
(-0.5)
P 		45.2
(-2.6)
P 		44
B 73
(2.7)
P 		53.8
(-0.3)
P 		36.9
(-3)
P 		52.5
(-0.5)
P 		75
(3)
P 		70.6
(2.3)
P 		38
C 85.3
(3)
P 		58.8
(0.1)
P 		56.4
(-0.2)
P 		30.5
(-3)
P 		47.6
(-1.1)
P 		47
(-1.2)
P 		55
avr 77.1
(3) 		66.4
(1) 		45.1
(-2.9) 		44.8
(-3) 		61.1
(0) 		54.3
(-1.2) 		32
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176856_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5136
Sequence Type Exemplar sequence
Representative Public ID T27F7.3 NCBI
Target Description T27F7.3 /REP_DB=WormBase Gene ID /WP=CE07559 /CHR=2 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG01561.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062517(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062517 NCBI Caenorhabditis elegans T27F7.3a (T27F7.3) mRNA, complete cds. 11/11 A 
SNAP00000006642 ENSEMBL cdna:SNAP chromosome:CEL140:II:4991320:4993062:-1 11/11 None
GENEFINDER00000006646 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4991572:4993062:-1 11/11 None
T27F7.3a ENSEMBL cdna:known chromosome:CEL140:II:4990816:4993565:-1 gene:T27F7.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
266853 2 1 NM_062517 176856_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4991317-4993534(-) 100.0 100.0
Public Domain and Genome References
Ensembl T27F7.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_494918 T27F7.3a [Caenorhabditis elegans] gb|AAK39304.2| Hypothetical protein T27F7.3a [Caenorhabditis elegans] emb|CAD24083.1| putative alpha 2 mannosyltransferase [Caenorhabditis elegans] 0.0
blast CAE59016 Hypothetical protein CBG02293 [Caenorhabditis briggsae] 0.0
blast CAF99570 unnamed protein product [Tetraodon nigroviridis] 2.0E-63
Protein Domains
Database ID Description E-Value
Pfam IPR005599 EMBL-EBI Plasmid Maintenance Protein 5.3E-57
Pfam IPR005599 EMBL-EBI Plasmid Maintenance Protein 8.9E-102
Pfam IPR001950 EMBL-EBI Translation initiation factor SUI1 1.9E-11
Pfam IPR005599 EMBL-EBI Plasmid Maintenance Protein 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494918.2 7 7-26,167-189,202-224,256-278,285-304,309-326,335-357
T27F7.3a 7 7-26,167-189,202-224,256-278,285-304,309-326,335-357
SNAP00000006642 7 7-26,130-152,165-187,219-241,248-267,272-289,298-320
GENEFINDER00000006646 7 7-26,167-189,202-224,256-278,285-304,309-326,335-357
Sequence
Target Sequence 
>C. ELEGANS:176856_AT
gtcgtttttcgttgtatttctgtctaccttgttgcttcgtggttcgttcccgacgaagtt
taccagtctgctgaagttgctcaccatctcgtctatggcactggtcacctttcgtgggag
tggcgccactcgctacgttcattcttccaccctgctttgattgctttcaatgtcgtgcaa
ggaggaagtgctttgtttggagttcatccatggtactggtatatcgtttctggaatacct
gcagtgctcactgttcaaatgattcccatcatcgtcggacttctcggcccaaacatattc
cgaccgtcgctgcttccattttttgctacgatcacctacatcattgttcactctttgctt
ccgcacaaagagcaacgtttcctcctcccaatcattccactactttgtatttatgcagga
ggagcatttcaaaatttgaagaagtggcgaggaagtgctatggctgttatgatcgcaata
aacataggaatcgcactattcacatctcgatatca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCGTTTTTCGTTGTATTTCTGTCT 503 473 238 Antisense
GTTCCCGACGAAGTTTACCAGTCTG 593 439 283 Antisense
TCACCATCTCGTCTATGGCACTGGT 85 627 318 Antisense
CTGGAATACCTGCAGTGCTCACTGT 439 241 467 Antisense
GACTTCTCGGCCCAAACATATTCCG 550 371 515 Antisense
TCGCTGCTTCCATTTTTTGCTACGA 23 601 544 Antisense
TTTGCTACGATCACCTACATCATTG 333 669 559 Antisense
TACATCATTGTTCACTCTTTGCTTC 188 645 574 Antisense
GCACAAAGAGCAACGTTTCCTCCTC 312 317 600 Antisense
CTCCCAATCATTCCACTACTTTGTA 619 233 622 Antisense
TCGCACTATTCACATCTCGATATCA 125 601 728 Antisense
Sequence Source Affymetrix Proprietary Database