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[176855_s_at]

Description Y67H2A.A /REP_DB=WormBase Gene ID /WP=CE24571 /CHR=4 /FEA=Sanger Annotation /DEF=(HINXTON) protein_id:CAB61133.1
Cluster 6grp_total > 26      4grp_total > 53      6grp_cgc > 14      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 127
1 2 3 4 range
A 137.8
(3)
A 		127.1
(2.3)
A 		69.5
(-1.7)
A 		50.4
(-3)
A 		87
B 88.2
(3)
A 		73.6
(1.6)
A 		46.5
(-0.9)
A 		23.4
(-3)
A 		65
C 150
(3)
A 		85.1
(-0.1)
A 		91.3
(0.2)
A 		25.1
(-3)
A 		125
avr 125.3
(3) 		95.3
(1) 		69.1
(-0.7) 		33
(-3) 		92

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 183
1 2 3 4 5 6 range
A 71.8
(-0.5)
A 		150.2
(3)
A 		39.1
(-2)
A 		35.7
(-2.1)
A 		16.3
(-3)
A 		108.8
(1.1)
A 		134
B 108.9
(3)
A 		102.2
(2.5)
A 		39.7
(-1.8)
A 		26.1
(-2.8)
A 		22.9
(-3)
A 		55.8
(-0.7)
A 		86
C 199.2
(3)
A 		114.2
(-0.2)
A 		52.9
(-2.4)
A 		68.4
(-1.9)
A 		87.5
(-1.2)
A 		38
(-3)
A 		161
avr 126.6
(3) 		122.2
(2.7) 		43.9
(-2.9) 		43.4
(-2.9) 		42.2
(-3) 		67.5
(-1.2) 		84
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 46
1 2 3 4 range
A 50.2
(3)
A 		27.6
(-0.2)
A 		18.2
(-1.5)
A 		7.4
(-3)
A 		43
B 28.1
(3)
A 		18.2
(0.4)
A 		10.8
(-1.5)
A 		5
(-3)
A 		23
C 49.6
(3)
A 		24.5
(-0.3)
A 		31.8
(0.6)
A 		4.2
(-3)
A 		45
avr 42.6
(3) 		23.4
(-0.1) 		20.3
(-0.6) 		5.5
(-3) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 69
1 2 3 4 5 6 range
A 13.2
(-2)
A 		53.4
(3)
A 		8.5
(-2.6)
A 		9.1
(-2.5)
A 		4.9
(-3)
A 		26.5
(-0.3)
A 		49
B 37.9
(3)
A 		34
(2.2)
A 		9.7
(-2.5)
A 		6.9
(-3)
A 		9.3
(-2.5)
A 		20.8
(-0.3)
A 		31
C 73.8
(3)
A 		34.8
(-0.8)
A 		13.5
(-2.9)
A 		13.8
(-2.9)
A 		28.3
(-1.5)
A 		12.7
(-3)
A 		61
avr 41.6
(3) 		40.7
(2.8) 		10.6
(-2.9) 		9.9
(-3) 		14.2
(-2.2) 		20
(-1.1) 		32
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176855_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19117
Sequence Type Exemplar sequence
Representative Public ID Y67H2A.A NCBI
Target Description Y67H2A.A /REP_DB=WormBase Gene ID /WP=CE24571 /CHR=4 /FEA=Sanger Annotation /DEF=(HINXTON) protein_id:CAB61133.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028094(11), NM_001028095(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028094 NCBI Caenorhabditis elegans UNCoordinated family member (unc-26) (unc-26) mRNA, complete cds. 11/11 None
NM_001028095 NCBI Caenorhabditis elegans UNCoordinated family member (unc-26) (unc-26) mRNA, complete cds. 11/11 None
SNAP00000010778 ENSEMBL cdna:SNAP chromosome:CEL140:IV:13265443:13282838:-1 11/11 None
GENEFINDER00000010795 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:13265462:13272480:-1 11/11 None
JC8.10b ENSEMBL cdna:known chromosome:CEL140:IV:13261526:13272483:-1 gene:JC8.10 11/11 A 
JC8.10a ENSEMBL cdna:known chromosome:CEL140:IV:13261526:13272483:-1 gene:JC8.10 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:13265467-13280715(-) 99.23 100.0
Public Domain and Genome References
Ensembl JC8.10
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAC44311 Hypothetical protein JC8.10a [Caenorhabditis elegans] emb|CAB05234.2| Hypothetical protein JC8.10a [Caenorhabditis elegans] ref|NP_001023265.1| UNCoordinated family member (unc-26) [Caenorhabditis elegans] gb|AAG18575.1| synaptojanin UNC-26B [Caenorhabditis elegans] 0.0
blast CAC70127 Hypothetical protein JC8.10b [Caenorhabditis elegans] emb|CAC70096.1| Hypothetical protein JC8.10b [Caenorhabditis elegans] ref|NP_001023266.1| UNCoordinated family member (unc-26) [Caenorhabditis elegans] gb|AAG18574.1| synaptojanin UNC-26A [Caenorhabditis elegans] 0.0
blast CAE58631 Hypothetical protein CBG01799 [Caenorhabditis briggsae] 0.0
blast CAC44310 Hypothetical protein Y67H2A.1 [Caenorhabditis elegans] ref|NP_502553.2| Y67H2A.1 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002013 EMBL-EBI Synaptojanin, N-terminal 5.1E-71
Pfam IPR005135 EMBL-EBI Endonuclease/exonuclease/phosphatase 1.8E-44
Pfam IPR005135 EMBL-EBI Endonuclease/exonuclease/phosphatase 1.8E-44
Pfam IPR002013 EMBL-EBI Synaptojanin, N-terminal 5.1E-71
Pfam IPR005135 EMBL-EBI Endonuclease/exonuclease/phosphatase 8.6E-6
Pfam IPR001279 EMBL-EBI Beta-lactamase-like 3.5E-21
Pfam IPR002013 EMBL-EBI Synaptojanin, N-terminal 3.4E-62
Pfam IPR005135 EMBL-EBI Endonuclease/exonuclease/phosphatase 8.6E-6
Sequence
Target Sequence 
>C. ELEGANS:176855_S_AT
cgccgtatcgcagcttgtcgagcgaagtcccgaaatcgccgaacctcaatccattaaaat
attcgttggaacttggaatgtgaatggaggaaagaatattcataatgtggcattccgtaa
tgaatcgagtctctcccactggatatttgccaattcaatgacacgtctcgtatctgtaga
agatgagcaactagctgatattgtagcaattggagttgaagaacttgttgatttgaatgc
aagtaatatggttaaagcaagtaccacaaatcaacgaatgtggtgtgaaagtattcgaaa
aactctttctgaaaaagctccatttgtgctcattggctccgagcagctcgtcggtgtttg
tctattcctcttcgcaagaccacgtgtctcaccatacctgaaagac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGCCGTATCGCAGCTTGTCGAGCGA 125 257 3288 Antisense
GAGCGAAGTCCCGAAATCGCCGAAC 197 387 3307 Antisense
TCGCCGAACCTCAATCCATTAAAAT 638 597 3323 Antisense
TCCGTAATGAATCGAGTCTCTCCCA 621 593 3401 Antisense
GTCTCTCCCACTGGATATTTGCCAA 582 467 3416 Antisense
TTGCCAATTCAATGACACGTCTCGT 81 701 3434 Antisense
GACACGTCTCGTATCTGTAGAAGAT 646 367 3447 Antisense
AAGCTCCATTTGTGCTCATTGGCTC 448 149 3602 Antisense
TTGTGCTCATTGGCTCCGAGCAGCT 46 689 3611 Antisense
CCGAGCAGCTCGTCGGTGTTTGTCT 684 263 3626 Antisense
ACGTGTCTCACCATACCTGAAAGAC 317 95 3669 Antisense
Sequence Source Affymetrix Proprietary Database