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[176847_at]

Description M01B12.2 /REP_DB=WormBase Gene ID /WP=CE18060 /GB=AAC17563.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 10      6grp_high1_10_10 > 0      4grp_highest_8_8 > 42      4grp_high_8_8 > 49      6grp_highest_nonorm > 90      6grp_total > 04      4grp_total > 62      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 109
1 2 3 4 range
A 197.4
(3)
P 		179.1
(1.7)
P 		164.4
(0.6)
P 		114.5
(-3)
P 		83
B 223.2
(3)
P 		139.5
(-2.4)
P 		166.2
(-0.6)
P 		129.5
(-3)
P 		94
C 197.9
(3)
P 		149.4
(-2.4)
P 		182
(1.2)
P 		143.9
(-3)
P 		54
avr 206.2
(3) 		156
(-0.9) 		170.9
(0.2) 		129.3
(-3) 		77

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 125
1 2 3 4 5 6 range
A 184.6
(3)
P 		157.3
(0.7)
P 		113.8
(-3)
P 		133.3
(-1.3)
P 		153
(0.3)
P 		136.2
(-1.1)
P 		71
B 185.6
(3)
P 		185
(3)
P 		153.3
(1)
P 		161.3
(1.5)
P 		90.1
(-3)
P 		121
(-1.1)
P 		96
C 154.5
(-0.1)
P 		132
(-1.3)
P 		98.7
(-3)
P 		215
(3)
P 		133.8
(-1.2)
P 		125.8
(-1.6)
P 		116
avr 174.9
(3) 		158.1
(1.1) 		121.9
(-3) 		169.9
(2.4) 		125.6
(-2.6) 		127.7
(-2.3) 		53
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 55
1 2 3 4 range
A 71.9
(3)
P 		38.9
(-0.6)
P 		43
(-0.1)
P 		16.8
(-3)
P 		55
B 71.2
(3)
P 		34.5
(-2.1)
P 		38.5
(-1.5)
P 		27.6
(-3)
P 		44
C 65.5
(3)
P 		42.9
(-0.3)
P 		63.4
(2.7)
P 		24.3
(-3)
P 		41
avr 69.5
(3) 		38.8
(-1) 		48.3
(0.3) 		22.9
(-3) 		47

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 40
1 2 3 4 5 6 range
A 34
(-1.2)
P 		55.9
(3)
P 		24.8
(-3)
P 		33.8
(-1.3)
P 		46.2
(1.1)
P 		33.2
(-1.4)
P 		31
B 64.6
(3)
P 		61.6
(2.4)
P 		37.2
(-2.9)
P 		42.7
(-1.7)
P 		36.6
(-3)
P 		45.1
(-1.2)
P 		28
C 57.3
(3)
P 		40.3
(-0.2)
P 		25.1
(-3)
P 		43.2
(0.4)
P 		43.3
(0.4)
P 		42.1
(0.2)
P 		32
avr 52
(2.8) 		52.6
(3) 		29
(-3) 		39.9
(-0.2) 		42
(0.3) 		40.1
(-0.2) 		24
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176847_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.268
Sequence Type Exemplar sequence
Representative Public ID M01B12.2 NCBI
Target Description M01B12.2 /REP_DB=WormBase Gene ID /WP=CE18060 /GB=AAC17563.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058696(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058696 NCBI Caenorhabditis elegans Y54E10BR.1 (Y54E10BR.1) mRNA, complete cds. 11/11 A 
SNAP00000040601 ENSEMBL cdna:SNAP chromosome:CEL140:I:3061462:3069251:1 11/11 None
GENEFINDER00000040606 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3062164:3069251:1 11/11 None
Y54E10BR.1 ENSEMBL cdna:known chromosome:CEL140:I:3059252:3069394:1 gene:Y54E10BR.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3062391-3069251(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y54E10BR.1
Entrez Gene 171881 Entrez gene
SwissProt Q9N3C5 EMBL-EBI
WormBase CE36244 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:258951_AT phosphatidylinositolglycan class N (PIG-N) family protein at
CANINE:1606104_AT phosphatidylinositol glycan, class N cfa
CANINE_2:CFA.3287.1.A1_AT phosphatidylinositol glycan, class N cfa
CANINE_2:CFAAFFX.1098.1.S1_AT phosphatidylinositol glycan, class N cfa
DROSOPHILA_2:1638995_AT dm
DROSGENOME1:146918_AT dm
CHICKEN:GGAAFFX.8161.1.S1_AT similar to Phosphatidylinositol glycan, class N gga
CHICKEN:GGAAFFX.8161.2.S1_S_AT similar to Phosphatidylinositol glycan, class N gga
HG-U133_PLUS_2:219048_AT phosphatidylinositol glycan, class N hs
HG-U133A:219048_AT phosphatidylinositol glycan, class N hs
HG-FOCUS:219048_AT phosphatidylinositol glycan, class N hs
HG-U133A_2:219048_AT phosphatidylinositol glycan, class N hs
HU35KSUBB:RC_AA033974_AT phosphatidylinositol glycan, class N hs
HU35KSUBB:RC_AA190634_AT phosphatidylinositol glycan, class N hs
HU35KSUBC:RC_Z41050_S_AT phosphatidylinositol glycan, class N hs
HU35KSUBD:RC_AA398154_AT phosphatidylinositol glycan, class N hs
U133_X3P:G6912499_3P_AT phosphatidylinositol glycan, class N hs
U133_X3P:HS.306402.0.S1_3P_S_AT phosphatidylinositol glycan, class N hs
HG-U133_PLUS_2:232101_S_AT phosphatidylinositol glycan, class N hs
HG-U133B:232101_S_AT phosphatidylinositol glycan, class N hs
HG-U95B:43331_AT phosphatidylinositol glycan, class N hs
HG-U95B:47408_I_AT phosphatidylinositol glycan, class N hs
HG-U95B:47409_R_AT phosphatidylinositol glycan, class N hs
HG-U95B:52513_AT phosphatidylinositol glycan, class N hs
HG-U95E:72596_AT phosphatidylinositol glycan, class N hs
HG-U95C:61830_R_AT Phosphatidylinositol glycan, class N hs
HG-U95C:63898_F_AT Phosphatidylinositol glycan, class N hs
HG-U95D:67120_AT Phosphatidylinositol glycan, class N hs
HU35KSUBD:RC_R05483_AT Phosphatidylinositol glycan, class N hs
MOUSE430_2:1425997_A_AT phosphatidylinositol glycan, class N mm
MOE430A:1425997_A_AT phosphatidylinositol glycan, class N mm
MOUSE430A_2:1425997_A_AT phosphatidylinositol glycan, class N mm
MOUSE430_2:1432115_A_AT phosphatidylinositol glycan, class N mm
MOE430A:1432115_A_AT phosphatidylinositol glycan, class N mm
MOUSE430A_2:1432115_A_AT phosphatidylinositol glycan, class N mm
MG-U74AV2:92296_AT phosphatidylinositol glycan, class N mm
MOUSE430_2:1421284_AT phosphatidylinositol glycan, class N mm
MOE430A:1421284_AT phosphatidylinositol glycan, class N mm
MOUSE430A_2:1421284_AT phosphatidylinositol glycan, class N mm
MOE430A:1425813_AT phosphatidylinositol glycan, class N mm
MOUSE430A_2:1425813_AT phosphatidylinositol glycan, class N mm
MOUSE430_2:1425813_AT phosphatidylinositol glycan, class N mm
MG-U74BV2:113764_AT Phosphatidylinositol glycan, class N, mRNA (cDNA clone MGC:13757 IMAGE:4024793) mm
MOE430B:1435262_AT Phosphatidylinositol glycan, class N, mRNA (cDNA clone MGC:13757 IMAGE:4024793) mm
MOUSE430_2:1435262_AT Phosphatidylinositol glycan, class N, mRNA (cDNA clone MGC:13757 IMAGE:4024793) mm
RAE230A:1376731_AT Phosphatidylinositol glycan, class N (predicted) rn
RAT230_2:1376731_AT Phosphatidylinositol glycan, class N (predicted) rn
RAE230B:1383604_AT Phosphatidylinositol glycan, class N (predicted) rn
RAT230_2:1383604_AT Phosphatidylinositol glycan, class N (predicted) rn
RAE230B:1383640_AT Phosphatidylinositol glycan, class N (predicted) rn
RAT230_2:1383640_AT Phosphatidylinositol glycan, class N (predicted) rn
RG-U34B:RC_AI009021_AT Phosphatidylinositol glycan, class N (predicted) rn
RG-U34C:RC_AI071177_AT Phosphatidylinositol glycan, class N (predicted) rn
YEAST_2:1771206_AT Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
9401 phosphoenolpyruvate-dependent sugar phosphotransferase system inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5351 sugar porter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF59557 Hypothetical protein Y54E10BR.1 [Caenorhabditis elegans] ref|NP_491097.3| Y54E10BR.1 [Caenorhabditis elegans] 0.0
blast CAE60571 Hypothetical protein CBG04200 [Caenorhabditis briggsae] 0.0
blast AAF59557 Hypothetical protein Y54E10BR.1 [Caenorhabditis elegans] ref|NP_491097.3| Y54E10BR.1 [Caenorhabditis elegans] 1.0E-131
blast CAE60571 Hypothetical protein CBG04200 [Caenorhabditis briggsae] 1.0E-126
blast XP_684789 PREDICTED: similar to Phosphatidylinositol glycan, class N [Danio rerio] 1.0E-125
Protein Domains
Database ID Description E-Value
Pfam IPR007070 EMBL-EBI Phosphatidylinositolglycan class N (PIG-N) 1.7E-25
Pfam IPR007070 EMBL-EBI Phosphatidylinositolglycan class N (PIG-N) 1.7E-25
Pfam IPR007070 EMBL-EBI Phosphatidylinositolglycan class N (PIG-N) 1.7E-25
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491097.3 12 5-27,415-437,444-466,519-541,548-570,585-607,627-646,694-716,754-776,791-813,826-845,860-882
Y54E10BR.1 12 5-27,415-437,444-466,519-541,548-570,585-607,627-646,694-716,754-776,791-813,826-845,860-882
SNAP00000040601 12 100-122,132-154,205-227,232-254,267-289,309-331,340-362,377-399,433-455,470-492,505-524,539-561
GENEFINDER00000040606 9 254-276,339-361,390-412,421-443,458-480,514-536,551-573,586-605,620-642
Sequence
Target Sequence 
>C. ELEGANS:176847_AT
tcctaactctattcgttcgcttcgaattcggacatctctctgacgttgagctcttgcagc
tgaaagttgattcaacgaaaatttcaaccggagaatacgtggagcttcgccgaacggtcg
tctgcgtctccttcgtcctgtgccctctcttcggcaccggaaactttgcatcaatcaatt
cattcaatccatccactttgaatctgttcatttcggtgttcagcccatttacaatggcta
ttctgttgatcttaaaacttcttattccgattcttctcgtcacatcggcttttgcttcca
tagttcgatttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCTAACTCTATTCGTTCGCTTCGA 232 593 1097 Antisense
TTCGCTTCGAATTCGGACATCTCTC 687 695 1112 Antisense
GACATCTCTCTGACGTTGAGCTCTT 88 371 1127 Antisense
GACGTTGAGCTCTTGCAGCTGAAAG 149 377 1138 Antisense
AGAATACGTGGAGCTTCGCCGAACG 46 73 1188 Antisense
CTCTCTTCGGCACCGGAAACTTTGC 213 229 1241 Antisense
AATCCATCCACTTTGAATCTGTTCA 489 167 1282 Antisense
TCATTTCGGTGTTCAGCCCATTTAC 551 619 1304 Antisense
AATGGCTATTCTGTTGATCTTAAAA 517 161 1329 Antisense
TCTTAAAACTTCTTATTCCGATTCT 289 611 1346 Antisense
GCTTTTGCTTCCATAGTTCGATTTG 50 305 1384 Antisense
Sequence Source Affymetrix Proprietary Database