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[176827_at]

Description Y97E10B.7 /REP_DB=WormBase Gene ID /WP=CE26327 /GB=AAK71415.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 21      4grp_total > 47      6grp_cgc > 72      6grp_cgc_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 115
1 2 3 4 range
A 92
(3)
A 		79.9
(2.1)
A 		83.6
(2.4)
A 		10.9
(-3)
A 		81
B 105.4
(3)
A 		9
(-3)
A 		13.5
(-2.7)
A 		11.5
(-2.8)
A 		96
C 124
(3)
A 		16.3
(-3)
A 		30.8
(-2.2)
A 		92.7
(1.3)
A 		108
avr 107.1
(3) 		35.1
(-3) 		42.6
(-2.4) 		38.4
(-2.7) 		72

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 166
1 2 3 4 5 6 range
A 135.9
(0.9)
A 		183.9
(3)
A 		123
(0.3)
A 		144.8
(1.3)
A 		47
(-3)
A 		47.7
(-3)
A 		137
B 112
(3)
A 		102.2
(1.9)
P 		106.6
(2.4)
A 		91
(0.6)
A 		94.3
(0.9)
P 		60.4
(-3)
A 		52
C 73.3
(-2.2)
A 		206.3
(2.8)
A 		212.5
(3)
A 		115.9
(-0.6)
A 		66.2
(-2.5)
A 		52.4
(-3)
A 		160
avr 107.1
(-0.1) 		164.1
(3) 		147.4
(2.1) 		117.2
(0.5) 		69.2
(-2.1) 		53.5
(-3) 		111
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 39
1 2 3 4 range
A 33.5
(3)
A 		17.3
(-0)
A 		21.9
(0.8)
A 		1.6
(-3)
A 		32
B 33.6
(3)
A 		2.2
(-3)
A 		3.1
(-2.8)
A 		2.5
(-2.9)
A 		31
C 41
(3)
A 		4.7
(-3)
A 		10.7
(-2)
A 		15.6
(-1.2)
A 		36
avr 36
(3) 		8.1
(-2.7) 		11.9
(-1.9) 		6.6
(-3) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 54
1 2 3 4 5 6 range
A 25.1
(-1.5)
A 		65.4
(3)
A 		26.8
(-1.3)
A 		36.7
(-0.2)
A 		14.2
(-2.7)
A 		11.6
(-3)
A 		54
B 39
(3)
A 		34
(1.2)
P 		25.9
(-1.8)
A 		24.1
(-2.4)
A 		38.3
(2.7)
P 		22.5
(-3)
A 		17
C 27.2
(-1.7)
A 		62.9
(3)
A 		54.1
(1.8)
A 		23.3
(-2.2)
A 		21.4
(-2.5)
A 		17.5
(-3)
A 		45
avr 30.4
(-0.9) 		54.1
(3) 		35.6
(-0) 		28
(-1.2) 		24.6
(-1.8) 		17.2
(-3) 		37
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176827_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11946
Sequence Type Exemplar sequence
Representative Public ID Y97E10B.7 NCBI
Target Description Y97E10B.7 /REP_DB=WormBase Gene ID /WP=CE26327 /GB=AAK71415.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072638(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072638 NCBI Caenorhabditis elegans Na/Ca eXchangers family member (ncx-10) (ncx-10) mRNA, complete cds. 11/11 None
SNAP00000041699 ENSEMBL cdna:SNAP chromosome:CEL140:V:7933066:7935613:-1 10/11 None
GENEFINDER00000041704 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:7933066:7937704:-1 10/11 None
Y97E10B.7 ENSEMBL cdna:known chromosome:CEL140:V:7933066:7935613:-1 gene:Y97E10B.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:7933066-7935614(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ncx-10
Ensembl Y97E10B.7
Entrez Gene 190821 Entrez gene
SwissProt Q965R5 EMBL-EBI
WormBase CE26327 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:250053_AT cation exchanger, putative (CAX8) at
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK71415 Na/ca exchangers protein 10 [Caenorhabditis elegans] ref|NP_505039.1| Na/Ca eXchangers family member (ncx-10) [Caenorhabditis elegans] 0.0
blast CAE64032 Hypothetical protein CBG08629 [Caenorhabditis briggsae] 0.0
blast AAB65309 Na/ca exchangers protein 9 [Caenorhabditis elegans] ref|NP_504342.1| Na/Ca eXchangers family member (ncx-9) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004837 EMBL-EBI Sodium/calcium exchanger membrane region 4.9E-29
Pfam IPR004837 EMBL-EBI Sodium/calcium exchanger membrane region 3.4E-17
Pfam IPR004837 EMBL-EBI Sodium/calcium exchanger membrane region 8.3E-22
Pfam IPR004837 EMBL-EBI Sodium/calcium exchanger membrane region 2.8E-29
Pfam IPR004837 EMBL-EBI Sodium/calcium exchanger membrane region 4.9E-29
Pfam IPR004837 EMBL-EBI Sodium/calcium exchanger membrane region 2.8E-29
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505039.1 12 99-121,175-197,210-229,234-256,398-415,430-452,459-481,501-523,528-550,578-600,612-634,638-660
Y97E10B.7 12 99-121,175-197,210-229,234-256,398-415,430-452,459-481,501-523,528-550,578-600,612-634,638-660
SNAP00000041699 12 99-121,175-197,206-228,253-272,366-383,398-420,427-449,469-491,496-518,528-550,563-585,589-611
GENEFINDER00000041704 13 15-37,145-167,221-243,256-275,280-302,444-461,476-498,505-527,547-569,574-596,606-628,641-663,667-689
Sequence
Target Sequence 
>C. ELEGANS:176827_AT
tattgagtcttcttgctctcaggttcactccgcttgacagggagcaaaagtattaccggg
aagtgtattcatacttaggatttttgatgtcaattgcctggatttatacaatttcttctg
aaatcatcaatgtcatcacaatgatcggcgtggcgactggagtctcacaggaaattcttg
gtttaaccatcatggcgtggagtaattgcattggagatattgtttctgacgtagctgttg
tgaaacaaggattccctaaaatggcgatggcagctgcgatcggaggaccactttttagta
agttgtactcgaggccctgtcaaataaaaataaccacttatatttcagatctccttattg
gatttggtctcccattcacaatcgcatgtttgcaaggaaaacaaattgatcttatcataa
caccggtctatcgtcttctcatgctatttttggcgatttctctattcacttcccttatcg
caatatttgttcaaaggttcaaggttcgatggccacatgcagttgcacttctca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATTGAGTCTTCTTGCTCTCAGGTT 656 663 1421 Antisense
GGTTCACTCCGCTTGACAGGGAGCA 339 507 1442 Antisense
ATGATCGGCGTGGCGACTGGAGTCT 90 45 1561 Antisense
AACCACTTATATTTCAGATCTCCTT 229 149 1752 Antisense
TCTCCCATTCACAATCGCATGTTTG 490 611 1788 Antisense
TATCATAACACCGGTCTATCGTCTT 188 659 1833 Antisense
TCGTCTTCTCATGCTATTTTTGGCG 420 603 1851 Antisense
TTTTGGCGATTTCTCTATTCACTTC 537 677 1868 Antisense
TATTCACTTCCCTTATCGCAATATT 689 663 1883 Antisense
GGTTCAAGGTTCGATGGCCACATGC 608 505 1916 Antisense
TGGCCACATGCAGTTGCACTTCTCA 496 543 1930 Antisense
Sequence Source Affymetrix Proprietary Database