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[176826_at]

Description Y40B10A.2 /REP_DB=WormBase Gene ID /WP=CE26078 /GB=AAK70657.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 83      6grp_high1_10_10 > 48      4grp_highest_8_8 > 21      4grp_high_8_8 > 15      6grp_highest_nonorm > 84      6grp_total > 38      4grp_total > 08      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 309
1 2 3 4 range
A 381.5
(-3)
P 		413.4
(-2.3)
P 		500
(-0.5)
P 		666.2
(3)
P 		285
B 407.2
(-2.3)
P 		387.3
(-3)
P 		461.4
(-0.4)
P 		560.5
(3)
P 		173
C 378.6
(-2.1)
P 		357.6
(-3)
P 		399.4
(-1.2)
P 		498.2
(3)
P 		141
avr 389.1
(-2.9) 		386.1
(-3) 		453.6
(-0.9) 		575
(3) 		189

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 919
1 2 3 4 5 6 range
A 635.5
(3)
P 		444.6
(0.1)
P 		484.6
(0.7)
P 		241
(-3)
P 		434.5
(-0.1)
P 		462.6
(0.4)
P 		395
B 1159.5
(3)
P 		633.5
(-0.7)
P 		399.5
(-2.3)
P 		299.2
(-3)
P 		598.6
(-0.9)
P 		846.2
(0.8)
P 		860
C 571.1
(-0.7)
P 		714.8
(0.7)
P 		348.6
(-3)
P 		416.6
(-2.3)
P 		402.5
(-2.5)
P 		937.8
(3)
P 		589
avr 788.7
(3) 		597.6
(0.6) 		410.9
(-1.8) 		318.9
(-3) 		478.5
(-1) 		748.9
(2.5) 		470
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 55
1 2 3 4 range
A 138.9
(3)
P 		89.7
(-3)
P 		130.7
(2)
P 		97.5
(-2)
P 		49
B 129.9
(3)
P 		95.7
(-3)
P 		106.9
(-1)
P 		119.4
(1.2)
P 		34
C 125.3
(1.5)
P 		102.8
(-1)
P 		139.2
(3)
P 		84
(-3)
P 		55
avr 131.4
(3) 		96.1
(-3) 		125.6
(2) 		100.3
(-2.3) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 342
1 2 3 4 5 6 range
A 117.1
(0.5)
P 		158.1
(3)
P 		105.5
(-0.3)
P 		61.1
(-3)
P 		131.1
(1.3)
P 		112.6
(0.2)
P 		97
B 403.4
(3)
P 		210.9
(-0.6)
P 		97
(-2.7)
P 		79.2
(-3)
P 		243.2
(0)
P 		315.5
(1.4)
P 		324
C 211.7
(0.3)
P 		218.1
(0.5)
P 		88.7
(-2.9)
P 		83.8
(-3)
P 		130.3
(-1.8)
P 		313.9
(3)
P 		230
avr 244.1
(2.9) 		195.7
(1.2) 		97.1
(-2.2) 		74.7
(-3) 		168.2
(0.3) 		247.3
(3) 		173
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176826_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15663
Sequence Type Exemplar sequence
Representative Public ID Y40B10A.2 NCBI
Target Description Y40B10A.2 /REP_DB=WormBase Gene ID /WP=CE26078 /GB=AAK70657.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071159(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071159 NCBI Caenorhabditis elegans Y40B10A.2 (Y40B10A.2) mRNA, complete cds. 11/11 None
SNAP00000009378 ENSEMBL cdna:SNAP chromosome:CEL140:V:2057496:2066737:1 11/11 None
GENEFINDER00000009383 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2057496:2060908:1 11/11 None
Y40B10A.2 ENSEMBL cdna:known chromosome:CEL140:V:2057496:2060908:1 gene:Y40B10A.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2059306-2060908(+) 94.09 100.0
Public Domain and Genome References
Gene Title
Ensembl Y40B10A.2
Entrez Gene 189785 Entrez gene
SwissProt Q965W3 EMBL-EBI
WormBase CE21796 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
8171 O-methyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK70657 Hypothetical protein Y40B10A.2 [Caenorhabditis elegans] ref|NP_503560.2| Y40B10A.2 [Caenorhabditis elegans] 1.0E-127
blast AAK70657 Hypothetical protein Y40B10A.2 [Caenorhabditis elegans] ref|NP_503560.2| Y40B10A.2 [Caenorhabditis elegans] 1.0E-115
blast CAE66789 Hypothetical protein CBG12149 [Caenorhabditis briggsae] 1.0E-109
blast AAK70661 Hypothetical protein Y40B10A.6 [Caenorhabditis elegans] ref|NP_503558.1| Y40B10A.6 [Caenorhabditis elegans] 1.0E-103
Protein Domains
Database ID Description E-Value
Pfam IPR002935 EMBL-EBI O-methyltransferase, family 3 3.7E-8
Pfam IPR002935 EMBL-EBI O-methyltransferase, family 3 5.2E-34
Pfam IPR002935 EMBL-EBI O-methyltransferase, family 3 3.7E-8
Pfam IPR002935 EMBL-EBI O-methyltransferase, family 3 5.2E-34
Sequence
Target Sequence 
>C. ELEGANS:176826_AT
actccaggctgagctcctcaaggagacactcgacaatgctccaatgtctatgatgctcgg
agcgccggaagttctcacgttcggacagaatttcatcagatcgttcggtggcaggcgtgt
cattgatgtcggtacctacaccggagcatctgccttggcatgggctctcgctgttccgga
tgatggagaagtgttcacttttgacattgatcacactaattataaaaagttcggagttcc
gatcatctcgaaatgcgagaagactttcaaaaagattcgtgctatcgaaggatccgcgtt
ggatggacttgacaagctcatcgccgacggacaatctggaacctttgattttgccttcat
cgatgccgacaaggtgaactacagtaactattacgatagagctgtcaccctgctccgcaa
gggaggtgtcatttttgtagacaatagtctttggaacggatccgtgtgcgatccagcgag
aagaaccgagccgagcaccgtcgcgatccaccaggcaaatgacaaaatcttcaaggatga
ccgtacctattctgctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTCCAGGCTGAGCTCCTCAAGGAG 601 97 72 Antisense
GAGACACTCGACAATGCTCCAATGT 309 393 94 Antisense
CAATGTCTATGATGCTCGGAGCGCC 468 183 113 Antisense
TCATCAGATCGTTCGGTGGCAGGCG 209 621 164 Antisense
GCAGGCGTGTCATTGATGTCGGTAC 419 311 182 Antisense
ATGTCGGTACCTACACCGGAGCATC 217 45 197 Antisense
GGAGTTCCGATCATCTCGAAATGCG 604 517 304 Antisense
ATTACGATAGAGCTGTCACCCTGCT 427 13 461 Antisense
GGAACGGATCCGTGTGCGATCCAGC 633 523 524 Antisense
GTCGCGATCCACCAGGCAAATGACA 330 475 571 Antisense
AAGGATGACCGTACCTATTCTGCTC 256 161 604 Antisense
Sequence Source Affymetrix Proprietary Database