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[176822_at] Calmodulin

Description C50C3.5 /REP_DB=WormBase Gene ID /WP=CE00121 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Calmodulin
Cluster 6grp_high1_10_10 > 90      4grp_highest_8_8 > 31      4grp_high_8_8 > 05      6grp_total > 0      4grp_total > 12      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 605
1 2 3 4 range
A 364.8
(-0.9)
P 		212.2
(-2.6)
P 		178.1
(-3)
P 		708
(3)
P 		530
B 342
(-1.2)
P 		271.5
(-2)
P 		187.7
(-3)
P 		701.2
(3)
P 		514
C 302.1
(-2.4)
P 		282.4
(-2.6)
P 		246.6
(-3)
P 		782.8
(3)
P 		536
avr 336.3
(-1.5) 		255.4
(-2.4) 		204.1
(-3) 		730.7
(3) 		527

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 160
1 2 3 4 5 6 range
A 181
(-3)
P 		271.7
(0.4)
P 		340.9
(3)
P 		296.3
(1.3)
P 		228.2
(-1.2)
P 		209.8
(-1.9)
P 		160
B 259.8
(-1.1)
P 		337.6
(3)
P 		285.3
(0.2)
P 		225.1
(-3)
P 		336.9
(3)
P 		281.6
(0)
P 		113
C 299.1
(2.1)
P 		311
(2.7)
P 		187.6
(-3)
P 		258.4
(0.3)
A 		318
(3)
P 		266.5
(0.6)
P 		130
avr 246.6
(-3) 		306.8
(3) 		271.3
(-0.5) 		259.9
(-1.7) 		294.4
(1.8) 		252.6
(-2.4) 		60
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 106
1 2 3 4 range
A 132.8
(3)
P 		46.1
(-3)
P 		46.6
(-3)
P 		103.6
(1)
P 		87
B 109
(0.7)
P 		67
(-1.7)
P 		43.5
(-3)
P 		149.4
(3)
P 		106
C 100
(-0.8)
P 		81.2
(-3)
P 		85.9
(-2.4)
P 		132
(3)
P 		51
avr 113.9
(1.8) 		64.8
(-2.5) 		58.7
(-3) 		128.3
(3) 		70

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 104
1 2 3 4 5 6 range
A 33.4
(-3)
P 		96.6
(3)
P 		74.3
(0.9)
P 		75.2
(1)
P 		68.9
(0.4)
P 		51.1
(-1.3)
P 		63
B 90.4
(-0.6)
P 		112.4
(1.1)
P 		69.3
(-2.2)
P 		59.6
(-3)
P 		136.9
(3)
P 		105
(0.5)
P 		77
C 110.9
(3)
P 		94.9
(1.5)
P 		47.8
(-3)
P 		52
(-2.6)
A 		102.9
(2.2)
P 		89.2
(0.9)
P 		63
avr 78.2
(-0.7) 		101.3
(2.8) 		63.8
(-2.8) 		62.3
(-3) 		102.9
(3) 		81.8
(-0.1) 		41
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176822_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7181
Sequence Type Exemplar sequence
Representative Public ID C50C3.5 NCBI
Target Description C50C3.5 /REP_DB=WormBase Gene ID /WP=CE00121 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Calmodulin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066385(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066385 NCBI Caenorhabditis elegans C50C3.5 (Calmodulin) mRNA, complete cds. 11/11 None
GENEFINDER00000020239 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8179177:8180218:1 10/11 None
C50C3.5 ENSEMBL cdna:known chromosome:CEL140:III:8179177:8181485:1 gene:C50C3.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000020234 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8179172-8181481(+) 100.0 100.0
Public Domain and Genome References
Gene Title Calmodulin
Ensembl C50C3.5
Entrez Gene 183642 Entrez gene
SwissProt P34368 EMBL-EBI
WormBase CE00121 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA27976 Hypothetical protein C50C3.5 [Caenorhabditis elegans] ref|NP_498786.1| C50C3.5 [Caenorhabditis elegans] sp|P34368|YLJ5_CAEEL Hypothetical calcium-binding protein C50C3.5 in chromosome III 2.0E-87
blast AAA27976 Hypothetical protein C50C3.5 [Caenorhabditis elegans] ref|NP_498786.1| C50C3.5 [Caenorhabditis elegans] sp|P34368|YLJ5_CAEEL Hypothetical calcium-binding protein C50C3.5 in chromosome III 5.0E-76
blast CAE70195 Hypothetical protein CBG16669 [Caenorhabditis briggsae] 1.0E-50
blast CAE70195 Hypothetical protein CBG16669 [Caenorhabditis briggsae] 1.0E-35
blast AAC68892 VU91D calmodulin [synthetic construct] 1.0E-9
blast AAC68892 VU91D calmodulin [synthetic construct] 2.0E-9
Protein Domains
Database ID Description E-Value
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 6.6E-6
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 5.9E-6
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.31
Sequence
Target Sequence 
>C. ELEGANS:176822_AT
atgcccgtggcaaaagacaatcaattttgggatgcactgatggaaaataaagtggcgaaa
aagttgataaagaagcataaatgcaaggctaagtgtgaaaatattgatgatctggcaaat
agatatgaagtctccaagcaagaagttgaaaaggtgttcaaaattttccaattaatggat
gacgatggatcaggtacaatttcttcatctgaagttgccaaaatgttaaatgaattgggt
attgatgtttcaccaaaagttgttcaagctgtaatgagaagcagtgatgtcagtggagat
ggacaaattgattttgaagaatttctagctgctgttacttccaaaattaagcttagtacc
gtgaaagcagatgtacagttgatgctttcaaaaattgataacaatccagaaaaagtggta
agttggggaattgaacacttgatgattatattctgtgaagagccactcaagtcttgttac
tatagacttgctctgactg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCCCGTGGCAAAAGACAATCAAT 343 41 13 Antisense
GACAATCAATTTTGGGATGCACTGA 125 365 28 Antisense
GGATCAGGTACAATTTCTTCATCTG 700 511 199 Antisense
GTTTCACCAAAAGTTGTTCAAGCTG 586 449 259 Antisense
GAAGCAGTGATGTCAGTGGAGATGG 381 341 290 Antisense
GAATTTCTAGCTGCTGTTACTTCCA 687 325 331 Antisense
CTAGCTGCTGTTACTTCCAAAATTA 464 225 337 Antisense
GCAGATGTACAGTTGATGCTTTCAA 281 313 379 Antisense
TATTCTGTGAAGAGCCACTCAAGTC 165 661 461 Antisense
GAGCCACTCAAGTCTTGTTACTATA 81 385 472 Antisense
TGTTACTATAGACTTGCTCTGACTG 40 563 487 Antisense
Sequence Source Affymetrix Proprietary Database