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[176821_s_at]

Description Y50D7A.6 /REP_DB=WormBase Gene ID /WP=CE26143 /GB=AAK68513.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 29      4grp_total > 28      6grp_cgc > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 80
1 2 3 4 range
A 47.7
(-2.5)
A 		84
(0.6)
A 		42.2
(-3)
A 		112.7
(3)
A 		71
B 86.5
(3)
A 		61.2
(0.2)
A 		32.4
(-3)
A 		67.1
(0.8)
A 		54
C 87.1
(0.6)
A 		102.4
(2.6)
A 		59.9
(-3)
A 		105.3
(3)
A 		45
avr 73.8
(0.5) 		82.5
(1.5) 		44.8
(-3) 		95
(3) 		50

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 88
1 2 3 4 5 6 range
A 71.1
(3)
A 		40.4
(-0.1)
A 		10.8
(-3)
A 		15.8
(-2.5)
A 		50.3
(0.9)
A 		45.3
(0.4)
A 		60
B 90.1
(2.2)
A 		59.8
(-0.4)
A 		98.8
(3)
A 		41.6
(-2)
A 		77.7
(1.2)
P 		29.9
(-3)
A 		69
C 75.9
(3)
P 		50.9
(0.6)
A 		14.8
(-2.8)
A 		12.9
(-3)
A 		73.3
(2.8)
P 		46
(0.2)
A 		63
avr 79
(3) 		50.4
(-0.1) 		41.5
(-1) 		23.4
(-3) 		67.1
(1.7) 		40.4
(-1.2) 		56
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 22
1 2 3 4 range
A 17.4
(2.3)
A 		18.2
(3)
A 		11
(-3)
A 		16.5
(1.6)
A 		7
B 27.6
(3)
A 		15.1
(-0.7)
A 		7.5
(-3)
A 		14.3
(-1)
A 		20
C 28.8
(2.7)
A 		29.4
(3)
A 		20.9
(-1.4)
A 		17.8
(-3)
A 		12
avr 24.6
(3) 		20.9
(1.1) 		13.1
(-3) 		16.2
(-1.4) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 13.1
(2)
A 		14.4
(2.6)
A 		2.4
(-3)
A 		4
(-2.3)
A 		15.2
(3)
A 		11
(1)
A 		13
B 31.4
(2.9)
A 		19.9
(-0.4)
A 		24
(0.8)
A 		11
(-3)
A 		31.6
(3)
P 		11.2
(-2.9)
A 		21
C 28.1
(3)
P 		15.5
(0)
A 		3.8
(-2.7)
A 		2.6
(-3)
A 		23.7
(2)
P 		15.4
(0)
A 		26
avr 24.2
(3) 		16.6
(0.5) 		10.1
(-1.6) 		5.9
(-3) 		23.5
(2.8) 		12.5
(-0.8) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176821_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8148
Sequence Type Exemplar sequence
Representative Public ID Y50D7A.6 NCBI
Target Description Y50D7A.6 /REP_DB=WormBase Gene ID /WP=CE26143 /GB=AAK68513.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064777(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064777 NCBI Caenorhabditis elegans Kinesin-Like Protein family member (klp-20) (klp-20) mRNA, complete cds. 11/11 None
GENEFINDER00000021197 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:221418:235694:1 11/11 None
SNAP00000021185 ENSEMBL cdna:SNAP chromosome:CEL140:III:222109:230595:1 11/11 None
Y50D7A.6 ENSEMBL cdna:known chromosome:CEL140:III:221418:228837:1 gene:Y50D7A.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:221417-227565(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol klp-20
Ensembl Y50D7A.6
Entrez Gene 3565057 Entrez gene
SwissProt Q965T6 EMBL-EBI
WormBase CE26143 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.2266.1.S1_S_AT similar to Kinesin-like protein KIF3A (Microtubule plus end-directed kinesin motor 3A) cfa
DROSOPHILA_2:1628601_AT Kinesin-like protein at 64D dm
DROSGENOME1:143453_AT Kinesin-like protein at 64D dm
CHICKEN:GGAAFFX.4247.1.A1_AT kinesin family member 3A gga
HU35KSUBC:RC_W70205_AT kinesin family member 3A hs
U133_X3P:HS.43670.0.S1_3P_AT kinesin family member 3A hs
HG-FOCUS:213623_AT kinesin family member 3A hs
HG-U133A_2:213623_AT kinesin family member 3A hs
HG-U133_PLUS_2:213623_AT kinesin family member 3A hs
HG-U133A:213623_AT kinesin family member 3A hs
HG-U95AV2:39956_AT kinesin family member 3A hs
HG-U95D:85549_AT kinesin family member 3A hs
MOUSE430_2:1420375_AT kinesin family member 3A mm
MOE430A:1420375_AT kinesin family member 3A mm
MOUSE430A_2:1420375_AT kinesin family member 3A mm
MG-U74AV2:100398_AT kinesin family member 3A mm
MU11KSUBA:D12645_S_AT kinesin family member 3A mm
MG-U74BV2:115929_AT kinesin family member 3A mm
MG-U74AV2:161275_AT kinesin family member 3A mm
MOE430A:1449841_AT kinesin family member 3A mm
MOUSE430A_2:1449841_AT kinesin family member 3A mm
MOUSE430_2:1449841_AT kinesin family member 3A mm
MG-U74CV2:130184_AT Kinesin family member 3A (Kif3a), mRNA mm
MU19KSUBB:TC26270_S_AT Kinesin family member 3A (Kif3a), mRNA mm
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5875 microtubule associated complex inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3774 motor activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK68513 Kinesin-like protein protein 20 [Caenorhabditis elegans] ref|NP_497178.1| Kinesin-Like Protein family member (klp-20) [Caenorhabditis elegans] 0.0
blast AAF99087 KRP85 [Caenorhabditis elegans] 0.0
blast CAE69512 Hypothetical protein CBG15720 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
scop a.2.7.Methicillin resistance protein FemA probable tRNA-binding arm All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Methicillin resistance protein FemA probable tRNA-binding arm 2.59999990463257
Pfam IPR001752 EMBL-EBI Kinesin, motor region 1.0E-126
Pfam IPR001752 EMBL-EBI Kinesin, motor region 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000021197 2 680-702,898-920
SNAP00000021185 1 542-564
Sequence
Target Sequence 
>C. ELEGANS:176821_S_AT
caatccaaattccaggaggcccacctcagaactcaattggaagaacggacggctgtaaaa
gtggaaattgaggagaggtattcatctttacaagaggaagcctttgtaaaatccaagaaa
atcaaaaaagttagcaatgagctgaaggatgctcgagcggagcttaaggatttggaagag
gaccatcagaggcaggtggaagctatgttggatgatattcgccagttgagaaaagagctc
ctactaaatattgccataatcgatgagtacattccggtggagcacgtggagctaattgag
aaatatgtcagttggagtgaggagcacggagattggcagctcaaagcaattgcctatact
ggaaataatatgagagcctccgcgcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAATCCAAATTCCAGGAGGCCCACC 394 181 1414 Antisense
GGCCCACCTCAGAACTCAATTGGAA 275 541 1431 Antisense
GAGGAGAGGTATTCATCTTTACAAG 125 405 1483 Antisense
GCTGAAGGATGCTCGAGCGGAGCTT 517 295 1554 Antisense
GGAAGAGGACCATCAGAGGCAGGTG 655 531 1587 Antisense
GAGGCAGGTGGAAGCTATGTTGGAT 633 405 1602 Antisense
GAGCTCCTACTAAATATTGCCATAA 422 387 1648 Antisense
TGAGTACATTCCGGTGGAGCACGTG 82 567 1677 Antisense
AGTGAGGAGCACGGAGATTGGCAGC 207 59 1729 Antisense
GCAGCTCAAAGCAATTGCCTATACT 38 315 1749 Antisense
GAAATAATATGAGAGCCTCCGCGCC 304 363 1775 Antisense
Sequence Source Affymetrix Proprietary Database