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[176820_at]

Description R05D3.11 /REP_DB=WormBase Gene ID /WP=CE25069 /GB=AAK21437.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 84      6grp_high1_10_10 > 47      4grp_highest_8_8 > 51      4grp_high_8_8 > 32      6grp_highest_nonorm > 82      6grp_total > 47      4grp_total > 47      6grp_highest_sub > Cu      6grp_cgc > 17      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 244
1 2 3 4 range
A 414.3
(3)
P 		196.4
(-2.4)
P 		170.7
(-3)
P 		203.4
(-2.2)
P 		244
B 317.5
(3)
P 		222.5
(-2.6)
P 		216
(-3)
P 		231.4
(-2.1)
P 		102
C 337.5
(3)
P 		196.7
(-2.4)
P 		257.4
(-0.1)
P 		181.6
(-3)
P 		156
avr 356.4
(3) 		205.2
(-3) 		214.7
(-2.6) 		205.5
(-3) 		151

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 223
1 2 3 4 5 6 range
A 346.3
(2.1)
P 		268.8
(-3)
P 		295.6
(-1.2)
P 		300.2
(-0.9)
P 		328.8
(1)
P 		359.6
(3)
P 		91
B 491.3
(3)
P 		381.1
(-0.5)
P 		365.2
(-1)
P 		360
(-1.2)
P 		303.9
(-3)
P 		457.5
(1.9)
P 		187
C 351.1
(-0.5)
P 		423.7
(2.6)
P 		302.5
(-2.5)
P 		298.9
(-2.7)
P 		291.4
(-3)
P 		432.5
(3)
P 		141
avr 396.2
(1.9) 		357.9
(-0.2) 		321.1
(-2.3) 		319.7
(-2.4) 		308
(-3) 		416.5
(3) 		109
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 121
1 2 3 4 range
A 150.8
(3)
P 		42.6
(-2.4)
P 		44.6
(-2.3)
P 		29.8
(-3)
P 		121
B 101.3
(3)
P 		54.9
(-2.4)
P 		50
(-2.9)
P 		49.3
(-3)
P 		52
C 111.7
(3)
P 		56.6
(-1.1)
P 		89.7
(1.4)
P 		30.6
(-3)
P 		81
avr 121.3
(3) 		51.4
(-2) 		61.4
(-1.2) 		36.6
(-3) 		85

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 111
1 2 3 4 5 6 range
A 63.8
(-3)
P 		95.6
(2.4)
P 		64.4
(-2.9)
P 		76.1
(-0.9)
P 		99.2
(3)
P 		87.6
(1)
P 		35
B 171
(3)
P 		126.9
(-0.2)
P 		88.7
(-3)
P 		95.3
(-2.5)
P 		123.5
(-0.5)
P 		170.6
(3)
P 		82
C 130.1
(2)
P 		129.3
(1.9)
P 		77
(-1.8)
P 		60.1
(-3)
P 		94.3
(-0.6)
P 		144.8
(3)
P 		85
avr 121.6
(1.7) 		117.3
(1.2) 		76.7
(-3) 		77.2
(-2.9) 		105.7
(0) 		134.3
(3) 		58
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176820_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7574
Sequence Type Exemplar sequence
Representative Public ID R05D3.11 NCBI
Target Description R05D3.11 /REP_DB=WormBase Gene ID /WP=CE25069 /GB=AAK21437.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066447(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066447 NCBI Caenorhabditis elegans R05D3.11 (R05D3.11) mRNA, complete cds. 11/11 None
SNAP00000035981 ENSEMBL cdna:SNAP chromosome:CEL140:III:8375215:8387369:1 11/11 None
GENEFINDER00000035984 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8375215:8384235:1 11/11 None
R05D3.11 ENSEMBL cdna:known chromosome:CEL140:III:8375215:8380748:1 gene:R05D3.11 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8375210-8380543(+) 99.3 100.0
Public Domain and Genome References
Gene Title
Gene Symbol met-2
Ensembl R05D3.11
Entrez Gene 176183 Entrez gene
SwissProt P34544 EMBL-EBI
WormBase CE25069 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
16568 chromatin modification inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
18024 histone-lysine N-methyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK21437 Hypothetical protein R05D3.11 [Caenorhabditis elegans] ref|NP_498848.1| R05D3.11 [Caenorhabditis elegans] sp|P34544|YNCA_CAEEL Hypothetical protein R05D3.11 in chromosome III 0.0
blast S44861 DNA topoisomerase II - Caenorhabditis elegans 0.0
blast CAE71274 Hypothetical protein CBG18157 [Caenorhabditis briggsae] 0.0
blast AAM54162 Hypothetical protein R05D3.1 [Caenorhabditis elegans] ref|NP_498849.2| R05D3.1 [Caenorhabditis elegans] sp|P34534|TOP2M_CAEEL Putative DNA topoisomerase II, mitochondrial precursor 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001241 EMBL-EBI DNA topoisomerase II 3.4E-45
Pfam IPR007728 EMBL-EBI Pre-SET 1.5E-49
Pfam IPR001739 EMBL-EBI Methyl-CpG binding 1.1E-27
Pfam IPR003594 EMBL-EBI ATP-binding region, ATPase-like 1.8E-12
Pfam IPR001214 EMBL-EBI Nuclear protein SET 3.1E-37
Pfam IPR002205 EMBL-EBI DNA gyrase/topoisomerase IV, subunit A 6.1E-31
Pfam IPR002205 EMBL-EBI DNA gyrase/topoisomerase IV, subunit A 9.9E-10
Pfam IPR001241 EMBL-EBI DNA topoisomerase II 3.4E-45
Pfam IPR007728 EMBL-EBI Pre-SET 1.0E-49
Pfam IPR001739 EMBL-EBI Methyl-CpG binding 1.1E-27
Pfam IPR003594 EMBL-EBI ATP-binding region, ATPase-like 4.7E-11
Pfam IPR001214 EMBL-EBI Nuclear protein SET 3.1E-37
Pfam IPR007728 EMBL-EBI Pre-SET 1.0E-49
Pfam IPR001739 EMBL-EBI Methyl-CpG binding 1.1E-27
Pfam IPR001214 EMBL-EBI Nuclear protein SET 1.8E-34
Sequence
Target Sequence 
>C. ELEGANS:176820_AT
aatacttcgctgatttggacttgaaggataccgtggagctggaaaagggtcgcgaagatc
atgaaactgattttggttacggaggagacgagtcagattatgatgacgaagaaggaagtg
atggtgactccggtgatgatgtaatgaacaaaatggtgaaacgtcaagactcttcggaga
gtggtgaagaaacaaaacggctgacaagacagaaaagaaagcaatctaaaaaatccggta
aaggaggaagtgtggagaaagatgacaccactccaagagattcaatggaaaaggataata
ttgaaagtaaagacgaacccgttttcaattgggataagtattttgagccgtttccattgt
atgttatagatgcaaaacagagaggaaatcttggaagattcttgaatcactcttgcgatc
cgaatgtgcacgttcaacacgtcatgtacgatacgcatgatcttcgtcttccatgggtcg
cgtttttcacacgaaaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATACTTCGCTGATTTGGACTTGAA 646 171 3377 Antisense
GGACTTGAAGGATACCGTGGAGCTG 409 523 3393 Antisense
GATGGTGACTCCGGTGATGATGTAA 111 409 3496 Antisense
GGTGAAACGTCAAGACTCTTCGGAG 175 501 3531 Antisense
GAAAGATGACACCACTCCAAGAGAT 532 359 3633 Antisense
GTATTTTGAGCCGTTTCCATTGTAT 133 453 3714 Antisense
GATTCTTGAATCACTCTTGCGATCC 651 431 3773 Antisense
GCGATCCGAATGTGCACGTTCAACA 705 289 3791 Antisense
GTTCAACACGTCATGTACGATACGC 544 443 3808 Antisense
ACGATACGCATGATCTTCGTCTTCC 75 95 3824 Antisense
TGGGTCGCGTTTTTCACACGAAAAT 205 551 3850 Antisense
Sequence Source Affymetrix Proprietary Database