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[176812_at]

Description F47D12.1 /REP_DB=WormBase Gene ID /WP=CE24987 /GB=AAK20069.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 13      4grp_total > 46      6grp_cgc > 40      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 717
1 2 3 4 range
A 961.7
(3)
P 		537
(-1.5)
A 		465.2
(-2.3)
A 		397
(-3)
P 		565
B 893.2
(3)
P 		629.1
(-0.5)
A 		551.4
(-1.6)
A 		445.7
(-3)
P 		448
C 1113.5
(3)
P 		696.1
(-0.9)
A 		489.6
(-2.8)
A 		471.8
(-3)
P 		642
avr 989.5
(3) 		620.7
(-1) 		502.1
(-2.3) 		438.2
(-3) 		551

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 705
1 2 3 4 5 6 range
A 803.9
(3)
A 		776.3
(2.6)
A 		442.5
(-1.8)
A 		569
(-0.1)
A 		407.5
(-2.2)
A 		347.4
(-3)
A 		457
B 775.5
(3)
M 		671.8
(1.8)
A 		441.4
(-1)
A 		590
(0.8)
A 		274.8
(-3)
A 		294.4
(-2.8)
A 		501
C 573.5
(-0.3)
P 		959.2
(3)
A 		640.3
(0.3)
A 		583.2
(-0.2)
A 		314.7
(-2.5)
A 		254.7
(-3)
P 		705
avr 717.6
(2) 		802.4
(3) 		508.1
(-0.5) 		580.7
(0.4) 		332.3
(-2.6) 		298.8
(-3) 		504
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 311
1 2 3 4 range
A 350.2
(3)
P 		116.6
(-1.8)
A 		121.6
(-1.7)
A 		58.1
(-3)
P 		292
B 284.8
(3)
P 		155.4
(-1.1)
A 		127.8
(-2)
A 		95
(-3)
P 		190
C 368.6
(3)
P 		200.1
(-0.5)
A 		170.6
(-1.1)
A 		79.5
(-3)
P 		289
avr 334.5
(3) 		157.4
(-1.1) 		140
(-1.5) 		77.5
(-3) 		257

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 208
1 2 3 4 5 6 range
A 148.2
(-1)
A 		276.1
(3)
A 		96.4
(-2.6)
A 		144.3
(-1.1)
A 		123
(-1.8)
A 		84.6
(-3)
A 		192
B 269.8
(3)
M 		223.6
(1.3)
A 		107.2
(-3)
A 		156.3
(-1.2)
A 		111.6
(-2.8)
A 		109.8
(-2.9)
A 		163
C 212.6
(0.7)
P 		292.7
(3)
A 		163
(-0.8)
A 		117.3
(-2.1)
A 		101.8
(-2.5)
A 		85.3
(-3)
P 		207
avr 210.2
(1.1) 		264.1
(3) 		122.2
(-2) 		139.3
(-1.4) 		112.1
(-2.3) 		93.2
(-3) 		171
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176812_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6343
Sequence Type Exemplar sequence
Representative Public ID F47D12.1 NCBI
Target Description F47D12.1 /REP_DB=WormBase Gene ID /WP=CE24987 /GB=AAK20069.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027424(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027424 NCBI Caenorhabditis elegans G-protein-linked Acetylcholine Receptor family member (gar-2) (gar-2) mRNA, complete cds. 11/11 None
GENEFINDER00000002715 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:6317269:6320542:1 11/11 None
SNAP00000002708 ENSEMBL cdna:SNAP chromosome:CEL140:III:6318096:6320542:1 9/11 None
F47D12.1c ENSEMBL cdna:known chromosome:CEL140:III:6309317:6320784:1 gene:F47D12.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001027423 7/11 Cross Hyb Matching Probes None
NM_001027422 8/11 Cross Hyb Matching Probes None
NM_001027426 7/11 Cross Hyb Matching Probes None
F47D12.1b.1 7/11 Cross Hyb Matching Probes None
F47D12.1a 8/11 Cross Hyb Matching Probes None
F47D12.1b.2 7/11 Cross Hyb Matching Probes None
F47D12.1e 7/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
175893 5 1 NM_001027424 176812_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6309358-6320541(+) 100.0 100.0
Public Domain and Genome References
Ensembl F47D12.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM98012 G-protein-linked acetylcholine receptor protein 2, isoform c [Caenorhabditis elegans] ref|NP_001022595.1| G-protein-linked Acetylcholine Receptor family member (gar-2) [Caenorhabditis elegans] 0.0
blast AAL15152 G-protein-linked acetylcholine receptor GAR-2a [Caenorhabditis elegans] 0.0
blast AAL65783 G-protein-linked acetylcholine receptor protein 2, isoform a [Caenorhabditis elegans] ref|NP_001022593.1| G-protein-linked Acetylcholine Receptor family member (gar-2) [Caenorhabditis elegans] gb|AAL15153.1| G-protein-linked acetylcholine receptor GAR-2b [Caenorhabditis elegans] sp|Q09388|ACM2_CAEEL Probable muscarinic acetylcholine receptor gar-2 (G-protein linked acetylcholine receptor 2) 0.0
blast AAL15152 G-protein-linked acetylcholine receptor GAR-2a [Caenorhabditis elegans] 1.0E-152
Protein Domains
Database ID Description E-Value
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 6.7E-14
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 5.2E-16
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 1.9E-67
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001022595.1 7 10-32,44-66,81-102,123-145,172-194,590-612,627-649
F47D12.1c 7 10-32,44-66,81-102,123-145,172-194,590-612,627-649
GENEFINDER00000002715 3 25-47,406-428,443-465
SNAP00000002708 2 259-281,296-318
Sequence
Target Sequence 
>C. ELEGANS:176812_AT
aaaggtcccattaatcgcagtttcgagagtggaaagtgtaaaaagtacagctggtggaaa
agttcgtcgattgataacccaaatgcgaagtcacagtattcgaagcaaacggaaagcgaa
taaaaataaagtgagcacttgttctccaaagagcacaacatcccttctcctcccaccttc
tacgtcatcaaccacccgtgaacggacacgccatccggtcacaattacactacaatttca
gagtgtcttatcggctcttaatttttttcaacgaaaaaaggtaacacgggaatacaaatc
tcgatctgagaaccgcgctcgaaaagctctacgtacaatcactttcatcttgggatcttt
tatcatactctggactcccttctacgttttggctacaatttatggattctgtgagacttg
taaagcttcaccatcattcaacacactttacacaatatcgtattacttgtgttatatgaa
ttcaccgctaaatccattctgctatgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAGGTCCCATTAATCGCAGTTTCG 213 121 1443 Antisense
TCCAAAGAGCACAACATCCCTTCTC 7 589 1587 Antisense
ATCAACCACCCGTGAACGGACACGC 211 23 1629 Antisense
CCATCCGGTCACAATTACACTACAA 637 271 1653 Antisense
AGTGTCTTATCGGCTCTTAATTTTT 538 53 1684 Antisense
TGAGAACCGCGCTCGAAAAGCTCTA 621 567 1749 Antisense
AGCTCTACGTACAATCACTTTCATC 526 79 1767 Antisense
ATCTTTTATCATACTCTGGACTCCC 674 31 1797 Antisense
TCCCTTCTACGTTTTGGCTACAATT 464 597 1818 Antisense
GTAAAGCTTCACCATCATTCAACAC 571 461 1862 Antisense
CACCGCTAAATCCATTCTGCTATGC 255 201 1925 Antisense
Sequence Source Affymetrix Proprietary Database