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[176810_s_at]

Description F45E4.3B /REP_DB=WormBase Gene ID /WP=CE24981 /GB=AAK29822.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 50      6grp_high1_10_10 > 16      4grp_highest_8_8 > 50      4grp_high_8_8 > 33      6grp_highest_nonorm > 60      6grp_total > 15      4grp_total > 46      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 894
1 2 3 4 range
A 1119
(3)
P 		402.7
(-2.8)
P 		380.9
(-3)
P 		376
(-3)
P 		743
B 1004.3
(3)
P 		581.7
(-0.7)
P 		387.2
(-2.5)
P 		327.9
(-3)
P 		676
C 1221.6
(3)
P 		437.4
(-2.3)
P 		370.8
(-2.7)
P 		329.3
(-3)
P 		892
avr 1115
(3) 		473.9
(-2) 		379.6
(-2.7) 		344.4
(-3) 		771

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 605
1 2 3 4 5 6 range
A 523.8
(1.2)
P 		657.6
(3)
P 		203.6
(-3)
P 		350.9
(-1.1)
P 		227.5
(-2.7)
P 		274
(-2.1)
P 		454
B 586.1
(3)
P 		449.7
(0.9)
P 		373.2
(-0.2)
P 		271.2
(-1.8)
P 		192.2
(-3)
P 		205.8
(-2.8)
P 		394
C 797.1
(3)
P 		406
(-1)
P 		489.8
(-0.2)
P 		335.1
(-1.8)
P 		225.1
(-2.9)
P 		216.2
(-3)
P 		581
avr 635.7
(3) 		504.4
(1.1) 		355.5
(-1) 		319.1
(-1.5) 		214.9
(-3) 		232
(-2.8) 		421
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 352
1 2 3 4 range
A 407.4
(3)
P 		87.4
(-2.4)
P 		99.6
(-2.2)
P 		55
(-3)
P 		352
B 320.3
(3)
P 		143.7
(-1.2)
P 		89.7
(-2.5)
P 		69.9
(-3)
P 		250
C 404.4
(3)
P 		125.7
(-1.8)
P 		129.2
(-1.7)
P 		55.5
(-3)
P 		349
avr 377.4
(3) 		118.9
(-1.9) 		106.2
(-2.1) 		60.1
(-3) 		317

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 251
1 2 3 4 5 6 range
A 96.6
(-1.3)
P 		233.9
(3)
P 		44.3
(-3)
P 		89
(-1.6)
P 		68.7
(-2.2)
P 		66.7
(-2.3)
P 		190
B 203.9
(3)
P 		149.7
(0.5)
P 		90.6
(-2.1)
P 		71.8
(-3)
P 		78.1
(-2.7)
P 		76.8
(-2.8)
P 		132
C 295.4
(3)
P 		123.9
(-1.5)
P 		124.7
(-1.5)
P 		67.4
(-3)
P 		72.8
(-2.9)
P 		72.4
(-2.9)
P 		228
avr 198.6
(3) 		169.2
(1.6) 		86.5
(-2.3) 		76.1
(-2.8) 		73.2
(-2.9) 		72
(-3) 		127
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176810_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8443
Sequence Type Exemplar sequence
Representative Public ID F45E4.3B NCBI
Target Description F45E4.3B /REP_DB=WormBase Gene ID /WP=CE24981 /GB=AAK29822.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068837(11), NM_068836(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068837 NCBI Caenorhabditis elegans F45E4.3b (F45E4.3) mRNA, complete cds. 11/11 None
NM_068836 NCBI Caenorhabditis elegans F45E4.3a (F45E4.3) mRNA, complete cds. 11/11 A 
SNAP00000024159 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7621026:7623536:1 11/11 None
GENEFINDER00000024180 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7621026:7623536:1 11/11 None
F45E4.3b.1 ENSEMBL cdna:known chromosome:CEL140:IV:7619614:7623536:1 gene:F45E4.3 11/11 None
F45E4.3b.2 ENSEMBL cdna:known chromosome:CEL140:IV:7619637:7623536:1 gene:F45E4.3 11/11 None
F45E4.3a ENSEMBL cdna:known chromosome:CEL140:IV:7621026:7628218:1 gene:F45E4.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7621031-7623542(+) 100.0 100.0
Public Domain and Genome References
Ensembl F45E4.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK29822 Hypothetical protein F45E4.3b [Caenorhabditis elegans] ref|NP_501238.1| F45E4.3b [Caenorhabditis elegans] 0.0
blast AAK29821 Hypothetical protein F45E4.3a [Caenorhabditis elegans] ref|NP_501237.1| F45E4.3a [Caenorhabditis elegans] 0.0
blast T25753 hypothetical protein F45E4.3 - Caenorhabditis elegans 0.0
blast CAE61926 Hypothetical protein CBG05923 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000008 EMBL-EBI C2 domain 4.2E-26
Sequence
Target Sequence 
>C. ELEGANS:176810_S_AT
gctcaacttcctcaaccaatgattccgattacaagatatgatgcaccgatgtccgatcct
tacgtgtcgagtagatctcgtcttgtggatcagatgaatcaaggttacaactccaatcaa
tatcatcatcattccacactgctcgcaccaaaatctgctcaaatgactcatattgctcca
acctataatcaacttgctcaacaacaaggaataccgatgcaaaacactcaaatggatcca
ttgatgatgtcttcaggtagaattccatcttcttcgtcgcaagtctactcaagaaatgag
atgaactacggaagtcgtccagctcagagtagtttattcgaatatggaaatcgccgacag
tatggagccgcaccacctccaacatatgatgttccaaatgtatcaagtgcctcggattgg
agaacaactcaacaacaaggattaatacaacaaatgccgatgcaaatgcaacaacaacaa
cataatacatttgatgctcgatggccaaaagaagatgctctctcaaggatgtatgcaact
gcttcacgaaggcgtgctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTCAACTTCCTCAACCAATGATTC 605 301 1924 Antisense
GATATGATGCACCGATGTCCGATCC 1 427 1958 Antisense
ATGTCCGATCCTTACGTGTCGAGTA 302 49 1972 Antisense
CTGCTCGCACCAAAATCTGCTCAAA 699 237 2062 Antisense
ATGACTCATATTGCTCCAACCTATA 474 45 2086 Antisense
TTCTTCGTCGCAAGTCTACTCAAGA 152 691 2193 Antisense
GAACTACGGAAGTCGTCCAGCTCAG 710 347 2226 Antisense
AAATCGCCGACAGTATGGAGCCGCA 401 119 2271 Antisense
CGCACCACCTCCAACATATGATGTT 198 255 2292 Antisense
AAGATGCTCTCTCAAGGATGTATGC 33 155 2435 Antisense
GCAACTGCTTCACGAAGGCGTGCTC 678 317 2458 Antisense
Sequence Source Affymetrix Proprietary Database