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[176808_at]

Description F14H12.8 /REP_DB=WormBase Gene ID /WP=CE07067 /GB=AAK68330.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 94      4grp_total > 18      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 118
1 2 3 4 range
A 11.3
(-3)
A 		14.9
(-2.8)
A 		29.2
(-2.1)
A 		129.3
(3)
A 		118
B 51
(-3)
A 		67.3
(0.2)
A 		52
(-2.8)
A 		81.4
(3)
A 		30
C 12.6
(-3)
A 		88.3
(3)
A 		17.8
(-2.6)
A 		78.9
(2.3)
A 		76
avr 25
(-3) 		56.8
(-0.3) 		33
(-2.3) 		96.5
(3) 		72

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 95
1 2 3 4 5 6 range
A 86.9
(2)
A 		13.3
(-2.7)
A 		36.5
(-1.2)
A 		8.9
(-3)
A 		101.7
(3)
A 		39.5
(-1)
A 		93
B 6.5
(-3)
A 		50.1
(1.7)
A 		29.3
(-0.5)
A 		50.8
(1.8)
A 		60.4
(2.8)
A 		61.9
(3)
A 		55
C 7.3
(-2.9)
A 		21.6
(-1.5)
A 		6.6
(-3)
A 		68.3
(3)
A 		62.7
(2.5)
A 		12.1
(-2.5)
A 		62
avr 33.6
(-1.9) 		28.3
(-2.5) 		24.1
(-3) 		42.7
(-0.8) 		74.9
(3) 		37.8
(-1.4) 		51
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 22
1 2 3 4 range
A 4.1
(-2.7)
A 		3.2
(-3)
A 		7.6
(-1.3)
A 		18.9
(3)
A 		16
B 16.3
(1.8)
A 		16.6
(2.1)
A 		12
(-3)
A 		17.4
(3)
A 		5
C 4.2
(-3)
A 		25.4
(3)
A 		6.2
(-2.4)
A 		13.3
(-0.4)
A 		21
avr 8.2
(-3) 		15.1
(2) 		8.6
(-2.7) 		16.5
(3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 16
(-0.1)
A 		4.7
(-2.5)
A 		7.9
(-1.8)
A 		2.3
(-3)
A 		30.7
(3)
A 		9.6
(-1.5)
A 		28
B 2.2
(-3)
A 		16.7
(0.9)
A 		7.1
(-1.7)
A 		13.5
(0)
A 		24.6
(3)
A 		23.1
(2.6)
A 		22
C 2.7
(-2.7)
A 		6.6
(-1.4)
A 		1.7
(-3)
A 		13.7
(0.9)
A 		20.3
(3)
A 		4.1
(-2.2)
A 		19
avr 7
(-2.6) 		9.3
(-1.9) 		5.6
(-3) 		9.8
(-1.7) 		25.2
(3) 		12.3
(-0.9) 		20
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176808_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16172
Sequence Type Exemplar sequence
Representative Public ID F14H12.8 NCBI
Target Description F14H12.8 /REP_DB=WormBase Gene ID /WP=CE07067 /GB=AAK68330.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076349(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076349 NCBI Caenorhabditis elegans F14H12.8 (F14H12.8) mRNA, complete cds. 11/11 None
F14H12.8 ENSEMBL cdna:known chromosome:CEL140:X:4362909:4364617:-1 gene:F14H12.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000002238 2/11 Cross Hyb Matching Probes None
GENEFINDER00000002244 2/11 Cross Hyb Matching Probes None
GENEFINDER00000002245 8/11 Cross Hyb Matching Probes None
SNAP00000002239 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4362909-4364618(-) 98.43 100.0
Public Domain and Genome References
Gene Title
Ensembl F14H12.8
Entrez Gene 184504 Entrez gene
SwissProt Q966K2 EMBL-EBI
WormBase CE07067 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68330 Hypothetical protein F14H12.8 [Caenorhabditis elegans] ref|NP_508750.1| F14H12.8 [Caenorhabditis elegans] 1.0E-103
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_508750.1 1 115-137
F14H12.8 1 115-137
Sequence
Target Sequence 
>C. ELEGANS:176808_AT
acaaaggaccacgacggatcaccagcaaatctgctccacgaaattgaagaagagaatcca
ttcgatttctacgcagaaccttctacaaccagttcaataccgttttcagctgttgtgcca
tcacctaccacaaatgaaccaaccacaaatctaccaaatacaacgcaaaatgcccaaaac
attgccgacaagcagcttgtacttgagtcccttctgctgcccgcagccgcattttctgga
tcccagaaatcgccgcaacgaaatttcgttgctcacatcattatttttttcgtggtcatg
ataatcctgttcgtgttcctgagcaacatcaatagcagctatgtccggcgagcaactaca
gaaaagttgcggaatattgatatggtggtaggaaatggttccttcatcgctaccatcatt
ctctacaaagcagctcatcgtgatgagcaggaaattgtcatcaatgtgaaaaaccgtcac
gaattggaaaatgcgcaattgggatacatcgacgtcatggaaaatgggcagattcaccgt
tttgccatca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACAAAGGACCACGACGGATCACCAG 614 111 94 Antisense
TTCTACGCAGAACCTTCTACAACCA 107 689 160 Antisense
CAACCAGTTCAATACCGTTTTCAGC 179 187 179 Antisense
TTTTCAGCTGTTGTGCCATCACCTA 162 677 196 Antisense
CAAGCAGCTTGTACTTGAGTCCCTT 138 159 282 Antisense
GAAATTTCGTTGCTCACATCATTAT 137 363 353 Antisense
TGATAATCCTGTTCGTGTTCCTGAG 674 563 392 Antisense
CAATAGCAGCTATGTCCGGCGAGCA 275 181 423 Antisense
AGGAAATGGTTCCTTCATCGCTACC 294 55 483 Antisense
ATCATTCTCTACAAAGCAGCTCATC 222 31 508 Antisense
GGGCAGATTCACCGTTTTGCCATCA 664 493 619 Antisense
Sequence Source Affymetrix Proprietary Database