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[176806_at]

Description F45E4.3A /REP_DB=WormBase Gene ID /WP=CE24980 /GB=AAK29821.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 51      6grp_high1_10_10 > 16      4grp_highest_8_8 > 58      4grp_high_8_8 > 33      6grp_highest_nonorm > 60      6grp_total > 16      4grp_total > 47      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 908
1 2 3 4 range
A 1169.9
(3)
P 		493
(-1.7)
P 		301.9
(-3)
P 		358.2
(-2.6)
P 		868
B 1149.6
(3)
P 		421
(-1.9)
P 		261.7
(-3)
P 		431.4
(-1.9)
P 		888
C 1069.2
(3)
P 		441.4
(-2.2)
P 		349.4
(-3)
P 		436.2
(-2.3)
P 		720
avr 1129.6
(3) 		451.8
(-1.9) 		304.3
(-3) 		408.6
(-2.2) 		825

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 514
1 2 3 4 5 6 range
A 659.8
(3)
P 		564.5
(1.6)
P 		251
(-2.9)
P 		273.2
(-2.6)
P 		324
(-1.8)
P 		244.3
(-3)
P 		416
B 719.4
(3)
P 		429.5
(-0.4)
P 		286.4
(-2.1)
P 		358
(-1.2)
P 		205.8
(-3)
P 		329.4
(-1.6)
P 		514
C 505.6
(2.2)
P 		550.3
(3)
P 		355.5
(-0.4)
P 		320.3
(-1)
P 		207
(-3)
P 		306.4
(-1.3)
P 		343
avr 628.3
(3) 		514.8
(1.2) 		297.6
(-2.2) 		317.2
(-1.9) 		245.6
(-3) 		293.4
(-2.3) 		383
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 374
1 2 3 4 range
A 426
(3)
P 		107
(-2.1)
P 		78.9
(-2.6)
P 		52.4
(-3)
P 		374
B 366.6
(3)
P 		104
(-2.1)
P 		60.6
(-3)
P 		91.9
(-2.4)
P 		306
C 353.9
(3)
P 		126.9
(-1.9)
P 		121.8
(-2)
P 		73.5
(-3)
P 		280
avr 382.2
(3) 		112.6
(-2.2) 		87.1
(-2.7) 		72.6
(-3) 		310

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 196
1 2 3 4 5 6 range
A 121.6
(-0.3)
P 		200.8
(3)
P 		54.7
(-3)
P 		69.3
(-2.4)
P 		97.8
(-1.2)
P 		59.5
(-2.8)
P 		146
B 250.3
(3)
P 		143
(-0.6)
P 		69.5
(-3)
P 		94.8
(-2.2)
P 		83.6
(-2.5)
P 		122.8
(-1.2)
P 		181
C 187.4
(3)
P 		167.9
(2)
P 		90.5
(-1.7)
P 		64.4
(-3)
P 		67
(-2.9)
P 		102.6
(-1.1)
P 		123
avr 186.4
(3) 		170.6
(2.2) 		71.6
(-3) 		76.2
(-2.8) 		82.8
(-2.4) 		95
(-1.8) 		115
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176806_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8407
Sequence Type Exemplar sequence
Representative Public ID F45E4.3A NCBI
Target Description F45E4.3A /REP_DB=WormBase Gene ID /WP=CE24980 /GB=AAK29821.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068836(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068836 NCBI Caenorhabditis elegans F45E4.3a (F45E4.3) mRNA, complete cds. 11/11 A 
SNAP00000024161 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7626334:7627989:1 11/11 None
GENEFINDER00000024179 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7623756:7627989:1 11/11 None
F45E4.3a ENSEMBL cdna:known chromosome:CEL140:IV:7621026:7628218:1 gene:F45E4.3 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177539 2 1 NM_068836 176806_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7621031-7627995(+) 100.0 100.0
Public Domain and Genome References
Ensembl F45E4.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK29821 Hypothetical protein F45E4.3a [Caenorhabditis elegans] ref|NP_501237.1| F45E4.3a [Caenorhabditis elegans] 0.0
blast T25753 hypothetical protein F45E4.3 - Caenorhabditis elegans 0.0
blast CAE61926 Hypothetical protein CBG05923 [Caenorhabditis briggsae] 0.0
blast AAK29821 Hypothetical protein F45E4.3a [Caenorhabditis elegans] ref|NP_501237.1| F45E4.3a [Caenorhabditis elegans] 1.0E-165
blast AAK29821 Hypothetical protein F45E4.3a [Caenorhabditis elegans] ref|NP_501237.1| F45E4.3a [Caenorhabditis elegans] 1.0E-164
blast T25753 hypothetical protein F45E4.3 - Caenorhabditis elegans 1.0E-158
Protein Domains
Database ID Description E-Value
Pfam IPR000008 EMBL-EBI C2 domain 4.2E-26
Pfam IPR000008 EMBL-EBI C2 domain 4.2E-26
Pfam IPR000008 EMBL-EBI C2 domain 4.2E-26
Sequence
Target Sequence 
>C. ELEGANS:176806_AT
tcatggccacattcaagtttctcttggttacgatggaaattctcgtcttgtcgccaaaat
cattcgagcaagaggattgaaatctcgtgatcaatctcgaagtgctcctaatccatttgt
taaagtgtatcttcttcctggaagaaaagtatctcataaacgaagaactcgctttgttga
ctcctcttgtgctcctgaatggaatcaagtacttgaatatcaagtagctccacataccct
taatacaatgttccttgaatttactgtatgtgattatcaacgagatgttgatgatcttcc
gttgggaaatgttcaaattccacttgctgataagtctgcaatcaacactggtcctcgatg
gtacccacttcaaggatcctacgatcaaccgattcctcagcattacatgaatggaacctc
gttatctcaaattccatcaatcgccgcagcttccaagcattctgcagccgtacacaacca
tcataatcataattattctgaagttcctccatcaattctttatccgaaaggaggtgtcgg
cacacgtcatccagacaaaccagtccgacatgcaacattcaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCATGGCCACATTCAAGTTTCTCTT 227 623 3081 Antisense
TGGAAATTCTCGTCTTGTCGCCAAA 36 549 3114 Antisense
CAATCTCGAAGTGCTCCTAATCCAT 150 181 3172 Antisense
ACGAAGAACTCGCTTTGTTGACTCC 67 93 3240 Antisense
TCAAGTAGCTCCACATACCCTTAAT 344 631 3300 Antisense
AATCAACACTGGTCCTCGATGGTAC 610 171 3420 Antisense
GTACCCACTTCAAGGATCCTACGAT 533 457 3441 Antisense
TCAACCGATTCCTCAGCATTACATG 454 631 3465 Antisense
ACCTCGTTATCTCAAATTCCATCAA 554 85 3496 Antisense
CGCAGCTTCCAAGCATTCTGCAGCC 647 253 3525 Antisense
ACCAGTCCGACATGCAACATTCAAC 693 85 3639 Antisense
Sequence Source Affymetrix Proprietary Database