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[176787_s_at]

Description F14H12.3 /REP_DB=WormBase Gene ID /WP=CE07063 /GB=AAK68325.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 90      6grp_high1_10_10 > 08      6grp_highest_nonorm > 50      6grp_total > 19      4grp_total > 50      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 716
1 2 3 4 range
A 197.8
(-1.6)
P 		694.5
(3)
P 		572.1
(1.9)
P 		43.8
(-3)
A 		651
B 208.5
(-1.1)
P 		638.9
(3)
P 		609.9
(2.7)
P 		13.7
(-3)
A 		625
C 160.5
(-2)
P 		729.8
(3)
P 		547.5
(1.4)
P 		43.6
(-3)
A 		686
avr 188.9
(-1.6) 		687.7
(3) 		576.5
(2) 		33.7
(-3) 		654

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 499
1 2 3 4 5 6 range
A 639.6
(3)
P 		433.4
(-1.4)
P 		355.9
(-3)
P 		389.7
(-2.3)
P 		576.3
(1.7)
P 		377.6
(-2.5)
P 		284
B 658.9
(3)
P 		339.6
(-2.5)
P 		313.6
(-3)
P 		384.3
(-1.8)
P 		520.2
(0.6)
P 		316.6
(-2.9)
P 		345
C 800.4
(3)
P 		323.3
(-2.7)
P 		420.4
(-1.6)
P 		424.7
(-1.5)
P 		658.4
(1.3)
P 		301.7
(-3)
P 		499
avr 699.6
(3) 		365.4
(-2.5) 		363.3
(-2.5) 		399.6
(-1.9) 		585
(1.1) 		332
(-3) 		368
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 207
1 2 3 4 range
A 72
(-0.3)
P 		150.7
(3)
P 		149.6
(3)
P 		6.4
(-3)
A 		144
B 66.5
(-0.5)
P 		157.8
(3)
P 		141.3
(2.4)
P 		2.9
(-3)
A 		155
C 53.1
(-1.6)
P 		209.8
(3)
P 		190.8
(2.4)
P 		7.3
(-3)
A 		203
avr 63.9
(-0.9) 		172.8
(3) 		160.6
(2.6) 		5.5
(-3) 		167

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 221
1 2 3 4 5 6 range
A 117.9
(-0.5)
P 		154.1
(1.8)
P 		77.5
(-3)
P 		98.8
(-1.7)
P 		173.9
(3)
P 		91.9
(-2.1)
P 		96
B 229.3
(3)
P 		113.1
(-1.6)
P 		76.1
(-3)
P 		101.8
(-2)
P 		211.3
(2.3)
P 		118.1
(-1.4)
P 		153
C 296.7
(3)
P 		98.6
(-2.6)
P 		107
(-2.4)
P 		85.4
(-3)
P 		213.1
(0.6)
P 		101
(-2.6)
P 		211
avr 214.6
(3) 		121.9
(-1.4) 		86.9
(-3) 		95.3
(-2.6) 		199.4
(2.3) 		103.7
(-2.2) 		128
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176787_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16606
Sequence Type Exemplar sequence
Representative Public ID F14H12.3 NCBI
Target Description F14H12.3 /REP_DB=WormBase Gene ID /WP=CE07063 /GB=AAK68325.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076344(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076344 NCBI Caenorhabditis elegans F14H12.3 (F14H12.3) mRNA, complete cds. 11/11 A 
SNAP00000002232 ENSEMBL cdna:SNAP chromosome:CEL140:X:4344262:4345552:1 11/11 None
GENEFINDER00000002243 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:4344262:4345552:1 11/11 None
F14H12.3 ENSEMBL cdna:known chromosome:CEL140:X:4344225:4345610:1 gene:F14H12.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4344262-4345553(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F14H12.3
Entrez Gene 180709 Entrez gene
SwissProt Q966K7 EMBL-EBI
WormBase CE07063 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68325 Hypothetical protein F14H12.3 [Caenorhabditis elegans] ref|NP_508745.1| F14H12.3 [Caenorhabditis elegans] 1.0E-122
blast CAE68561 Hypothetical protein CBG14396 [Caenorhabditis briggsae] 1.0E-102
blast AAH87548 Unknown (protein for IMAGE:3397679) [Xenopus laevis] 1.0E-17
Protein Domains
Database ID Description E-Value
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 1.6E-12
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 7.2E-11
Sequence
Target Sequence 
>C. ELEGANS:176787_S_AT
taggcaatgtagctgtggatcgggatgtactggaggctccgttgaacaagagccatgccc
tcaacaagctgcttgcccgtgcacaacatgcaatcaaccaccaccaccatgcaatacttg
taatacccaaccggttgtcattgtgactccagctccatgcacaacttgttaccaaccacc
agcgtgctcaacctgcgggcatgcacaacctttctatgatccatac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAGGCAATGTAGCTGTGGATCGGGA 691 647 504 Antisense
TCCGTTGAACAAGAGCCATGCCCTC 287 593 541 Antisense
CTTGCCCGTGCACAACATGCAATCA 277 219 575 Antisense
TGCACAACATGCAATCAACCACCAC 620 577 583 Antisense
CACCACCACCATGCAATACTTGTAA 296 201 602 Antisense
GCAATACTTGTAATACCCAACCGGT 81 323 614 Antisense
AACCGGTTGTCATTGTGACTCCAGC 510 145 632 Antisense
TCCAGCTCCATGCACAACTTGTTAC 127 589 651 Antisense
CATGCACAACTTGTTACCAACCACC 513 209 659 Antisense
GGGCATGCACAACCTTTCTATGATC 297 493 700 Antisense
TGCACAACCTTTCTATGATCCATAC 295 577 705 Antisense
Sequence Source Affymetrix Proprietary Database