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[176786_at]

Description F14H12.3 /REP_DB=WormBase Gene ID /WP=CE07063 /GB=AAK68325.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 90      6grp_high1_10_10 > 19      4grp_high_8_8 > 53      6grp_highest_nonorm > 81      6grp_total > 29      4grp_total > 58      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 334
1 2 3 4 range
A 183.3
(-0.9)
P 		338.3
(3)
P 		335.9
(2.9)
P 		102
(-3)
A 		236
B 207.2
(-0)
P 		252.8
(1.1)
P 		328.8
(3)
P 		86.9
(-3)
P 		242
C 214.9
(-1)
P 		420.8
(3)
P 		384
(2.3)
P 		108.8
(-3)
A 		312
avr 201.8
(-0.5) 		337.3
(2.7) 		349.6
(3) 		99.2
(-3) 		250

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 253
1 2 3 4 5 6 range
A 300.8
(3)
P 		271
(1)
P 		252.8
(-0.1)
P 		249.9
(-0.3)
P 		259.3
(0.3)
P 		209.2
(-3)
P 		92
B 305.5
(3)
P 		245.1
(0.4)
P 		242
(0.3)
P 		164.6
(-3)
P 		275.8
(1.7)
P 		194.2
(-1.7)
P 		141
C 417.1
(3)
P 		245
(-2.1)
P 		230.3
(-2.5)
P 		213.4
(-3)
P 		354.8
(1.2)
P 		218.7
(-2.8)
P 		204
avr 341.1
(3) 		253.7
(-0.9) 		241.7
(-1.5) 		209.3
(-2.9) 		296.6
(1) 		207.4
(-3) 		134
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 119
1 2 3 4 range
A 66.7
(1.3)
P 		73.4
(1.8)
P 		87.8
(3)
P 		14.9
(-3)
A 		73
B 66.1
(1.9)
P 		62.4
(1.6)
P 		76.2
(3)
P 		18.5
(-3)
P 		58
C 71.1
(-0.3)
P 		121
(2.3)
P 		133.8
(3)
P 		18.4
(-3)
A 		115
avr 68
(0.7) 		85.6
(2) 		99.3
(3) 		17.3
(-3) 		82

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 112
1 2 3 4 5 6 range
A 55.4
(-2.4)
P 		96.4
(3)
P 		55.1
(-2.4)
P 		63.4
(-1.4)
P 		78.2
(0.6)
P 		50.9
(-3)
P 		46
B 106.3
(2.5)
P 		81.6
(0.3)
P 		58.8
(-1.7)
P 		43.6
(-3)
P 		112
(3)
P 		72.4
(-0.5)
P 		68
C 154.6
(3)
P 		74.8
(-1.3)
P 		58.6
(-2.2)
P 		42.9
(-3)
P 		114.8
(0.9)
P 		73.2
(-1.4)
P 		112
avr 105.4
(3) 		84.3
(0.7) 		57.5
(-2.2) 		50
(-3) 		101.7
(2.6) 		65.5
(-1.3) 		55
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176786_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16606
Sequence Type Exemplar sequence
Representative Public ID F14H12.3 NCBI
Target Description F14H12.3 /REP_DB=WormBase Gene ID /WP=CE07063 /GB=AAK68325.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076344(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076344 NCBI Caenorhabditis elegans F14H12.3 (F14H12.3) mRNA, complete cds. 11/11 A 
SNAP00000002232 ENSEMBL cdna:SNAP chromosome:CEL140:X:4344262:4345552:1 11/11 None
GENEFINDER00000002243 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:4344262:4345552:1 11/11 None
F14H12.3 ENSEMBL cdna:known chromosome:CEL140:X:4344225:4345610:1 gene:F14H12.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4344262-4345553(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F14H12.3
Entrez Gene 180709 Entrez gene
SwissProt Q966K7 EMBL-EBI
WormBase CE07063 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68325 Hypothetical protein F14H12.3 [Caenorhabditis elegans] ref|NP_508745.1| F14H12.3 [Caenorhabditis elegans] 1.0E-122
blast CAE68561 Hypothetical protein CBG14396 [Caenorhabditis briggsae] 1.0E-102
blast AAH87548 Unknown (protein for IMAGE:3397679) [Xenopus laevis] 1.0E-17
Protein Domains
Database ID Description E-Value
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 1.6E-12
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 7.2E-11
Sequence
Target Sequence 
>C. ELEGANS:176786_AT
aactcgcactagactttgtccatccggcaactgccaaggaggttctacaacagaatcaaa
accatgcgtcctttatgatccacaaccaactcaaccacaatggggagcttggggaggttg
gagtagttgcagtgccacctgcggcggtggtacgatgacgagaagtcgtgtttgcaataa
cggatgctcaacctgccaatgtgtcggagctgctgccgagtcgcaagcttgcaatgctca
accgtgctgcacctggactgcttggtcttcctggtcggcgtgctcagtaacctgcggatc
tggaggatcaatcaccagatctaggcaatgtagctgtggatcgggatgtactggaggctc
cgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACTCGCACTAGACTTTGTCCATCC 471 141 183 Antisense
GTCCATCCGGCAACTGCCAAGGAGG 320 475 200 Antisense
GAATCAAAACCATGCGTCCTTTATG 409 325 235 Antisense
GTCCTTTATGATCCACAACCAACTC 669 475 250 Antisense
GTTGGAGTAGTTGCAGTGCCACCTG 534 435 299 Antisense
CTGCGGCGGTGGTACGATGACGAGA 509 235 321 Antisense
AATAACGGATGCTCAACCTGCCAAT 580 171 358 Antisense
CAACCTGCCAATGTGTCGGAGCTGC 538 185 371 Antisense
GCAAGCTTGCAATGCTCAACCGTGC 370 321 405 Antisense
GTGCTCAGTAACCTGCGGATCTGGA 202 479 462 Antisense
GATCGGGATGTACTGGAGGCTCCGT 437 411 521 Antisense
Sequence Source Affymetrix Proprietary Database