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[176785_at]

Description F53A10.2 /REP_DB=WormBase Gene ID /WP=CE24996 /GB=AAK71368.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 30      6grp_high1_10_10 > 24      4grp_highest_8_8 > 59      4grp_high_8_8 > 33      6grp_highest_nonorm > 61      6grp_total > 02      4grp_total > 46      6grp_highest_sub > De      6grp_high1_sub > 02      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 333
1 2 3 4 range
A 643.5
(3)
P 		601.3
(1.1)
P 		506.8
(-3)
P 		618.8
(1.9)
P 		137
B 708.8
(3)
P 		539.2
(-1.5)
P 		483.9
(-3)
P 		559.5
(-1)
P 		225
C 728.4
(3)
P 		477
(-1.5)
P 		457.9
(-1.9)
P 		395.4
(-3)
P 		333
avr 693.6
(3) 		539.2
(-1.4) 		482.9
(-3) 		524.6
(-1.8) 		211

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 415
1 2 3 4 5 6 range
A 518.3
(1.6)
P 		578.1
(3)
P 		436.7
(-0.2)
P 		407.8
(-0.9)
P 		467
(0.5)
P 		314.9
(-3)
P 		263
B 580
(3)
P 		551.5
(2.3)
P 		512.2
(1.2)
P 		485.3
(0.5)
P 		477.8
(0.3)
P 		349.4
(-3)
P 		231
C 511.2
(0.6)
P 		678.6
(3)
P 		479
(0.1)
P 		392
(-1.1)
P 		563.6
(1.3)
P 		264.1
(-3)
P 		415
avr 536.5
(1.6) 		602.7
(3) 		476
(0.4) 		428.4
(-0.6) 		502.8
(1) 		309.5
(-3) 		293
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 174
1 2 3 4 range
A 234.3
(3)
P 		130.5
(-1.3)
P 		132.5
(-1.3)
P 		90.6
(-3)
P 		144
B 226
(3)
P 		133.2
(-1.9)
P 		112.1
(-3)
P 		119.2
(-2.6)
P 		114
C 241.1
(3)
P 		137.1
(-0.6)
P 		159.6
(0.2)
P 		66.7
(-3)
P 		174
avr 233.8
(3) 		133.6
(-1.2) 		134.7
(-1.2) 		92.2
(-3) 		142

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 130
1 2 3 4 5 6 range
A 95.5
(-2.1)
P 		205.6
(3)
P 		95.1
(-2.1)
P 		103.4
(-1.8)
P 		140.9
(-0)
P 		76.7
(-3)
P 		129
B 201.8
(3)
P 		183.6
(1.6)
P 		124.4
(-3)
P 		128.5
(-2.7)
P 		194.1
(2.4)
P 		130.3
(-2.5)
P 		77
C 189.5
(2.2)
P 		207.1
(3)
P 		121.9
(-1)
P 		78.8
(-3)
P 		182.4
(1.8)
P 		88.4
(-2.6)
P 		128
avr 162.3
(0.8) 		198.8
(3) 		113.8
(-2.1) 		103.6
(-2.7) 		172.5
(1.4) 		98.5
(-3) 		100
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176785_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5787
Sequence Type Exemplar sequence
Representative Public ID F53A10.2 NCBI
Target Description F53A10.2 /REP_DB=WormBase Gene ID /WP=CE24996 /GB=AAK71368.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061527(11), NM_001027019(11), NM_182148(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061527 NCBI Caenorhabditis elegans F53A10.2a (F53A10.2) mRNA, complete cds. 11/11 None
NM_001027019 NCBI Caenorhabditis elegans F53A10.2c (F53A10.2) mRNA, complete cds. 11/11 None
NM_182148 NCBI Caenorhabditis elegans F53A10.2b (F53A10.2) mRNA, complete cds. 11/11 None
SNAP00000004722 ENSEMBL cdna:SNAP chromosome:CEL140:II:1225774:1230471:1 11/11 None
GENEFINDER00000004741 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:1225774:1230471:1 11/11 None
F53A10.2a ENSEMBL cdna:known chromosome:CEL140:II:1197380:1230695:1 gene:F53A10.2 11/11 None
F53A10.2c ENSEMBL cdna:known chromosome:CEL140:II:1224357:1230471:1 gene:F53A10.2 11/11 A 
F53A10.2b ENSEMBL cdna:known chromosome:CEL140:II:1227923:1230471:1 gene:F53A10.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:1197385-1230470(+) 97.13 100.0
Public Domain and Genome References
Ensembl F53A10.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK71368 Hypothetical protein F53A10.2a [Caenorhabditis elegans] ref|NP_493928.1| F53A10.2a [Caenorhabditis elegans] 0.0
blast AAO27840 Rap1GAP [Caenorhabditis elegans] 0.0
blast AAB66048 Hypothetical protein F53A10.2c [Caenorhabditis elegans] ref|NP_001022190.1| F53A10.2c [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000331 EMBL-EBI Rap/ran-GAP 2.5E-112
Pfam IPR003109 EMBL-EBI GoLoco 5.3E-10
Pfam IPR000331 EMBL-EBI Rap/ran-GAP 2.5E-112
Pfam IPR000331 EMBL-EBI Rap/ran-GAP 2.5E-112
Pfam IPR000331 EMBL-EBI Rap/ran-GAP 1.8E-81
Sequence
Target Sequence 
>C. ELEGANS:176785_AT
aagccggtcggtttctcaggaggctagtcacgtaccacaccgcgccgccacgattaatgt
gacgtcacggcctaaaaaatcgatatcctcgacgagttcgtcgaccagtgcccggacaca
ctcaccgattagggatgaggaggttgtcaagccatatagaaaagaatgggaaatttcatc
tcaagagtctccggataacgagcatgattcggatactggaatggaatccatgtcctccac
agaaatgtcaggtcaaactcgggcttcatcgtgcacattttgtgtagatgacttccacgt
ggcagcaaatcatcagtcctcagatgcaaagcgtctcgagaccctttgtgtagatgttac
acgacttcagaatgagaagcacgacttgttacgtcagaatgttagctgcaaaactgatat
taaaaagttaaaggatcgccaaagtgtgctctccgaggagctggaacgcgccaacgatga
gatttcgcggctgcggaggatgctgaaaaagccgtcaaacagcgatatggcgccggtcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGCCGGTCGGTTTCTCAGGAGGCT 12 149 1662 Antisense
TCAGGAGGCTAGTCACGTACCACAC 420 621 1677 Antisense
GCGCCGCCACGATTAATGTGACGTC 17 289 1703 Antisense
AATCCATGTCCTCCACAGAAATGTC 372 167 1886 Antisense
TCAGGTCAAACTCGGGCTTCATCGT 139 603 1909 Antisense
GTGTAGATGACTTCCACGTGGCAGC 65 487 1943 Antisense
ACGTGGCAGCAAATCATCAGTCCTC 699 95 1958 Antisense
CAGTCCTCAGATGCAAAGCGTCTCG 686 207 1975 Antisense
GCTCTCCGAGGAGCTGGAACGCGCC 308 299 2109 Antisense
GATTTCGCGGCTGCGGAGGATGCTG 696 427 2142 Antisense
CAAACAGCGATATGGCGCCGGTCCA 417 191 2177 Antisense
Sequence Source Affymetrix Proprietary Database