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[176778_s_at]

Description M01G12.12 /REP_DB=WormBase Gene ID /WP=CE23872 /GB=CAC35914.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 94      6grp_high1_10_10 > 58      6grp_highest_nonorm > 91      6grp_total > 48      4grp_total > 04      6grp_highest_sub > Cu      6grp_cgc > 18      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 559
1 2 3 4 range
A 222.4
(-0.8)
P 		88.6
(-2.2)
A 		13.7
(-3)
A 		572.9
(3)
P 		559
B 227
(-1.1)
P 		98
(-2.7)
A 		74.6
(-3)
A 		564.8
(3)
P 		490
C 239.1
(-0.6)
P 		100.6
(-2.5)
A 		59.3
(-3)
A 		517.4
(3)
P 		458
avr 229.5
(-0.8) 		95.7
(-2.4) 		49.2
(-3) 		551.7
(3) 		503

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 206
1 2 3 4 5 6 range
A 224.3
(1.1)
P 		224.6
(1.2)
P 		130.5
(-3)
P 		185.1
(-0.6)
P 		213
(0.6)
P 		266.5
(3)
P 		136
B 320.3
(2.5)
P 		217.5
(-0.8)
P 		154.4
(-2.9)
P 		150.9
(-3)
P 		200.8
(-1.4)
P 		336.2
(3)
P 		185
C 245.8
(0.2)
P 		280.5
(1.7)
P 		170.4
(-3)
P 		184.4
(-2.4)
P 		171.6
(-2.9)
P 		310
(3)
P 		140
avr 263.5
(1.4) 		240.9
(0.5) 		151.8
(-3) 		173.5
(-2.1) 		195.1
(-1.3) 		304.2
(3) 		152
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 117
1 2 3 4 range
A 81
(2.8)
P 		19.2
(-1.8)
A 		3.6
(-3)
A 		83.9
(3)
P 		80
B 72.4
(0.2)
P 		24.2
(-2.6)
A 		17.3
(-3)
A 		120.4
(3)
P 		103
C 79.1
(2.3)
P 		28.9
(-2.3)
A 		20.7
(-3)
A 		87.2
(3)
P 		67
avr 77.5
(1.6) 		24.1
(-2.3) 		13.9
(-3) 		97.2
(3) 		83

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 97
1 2 3 4 5 6 range
A 41.3
(-1.5)
P 		79.9
(3)
P 		28.4
(-3)
P 		46.9
(-0.8)
P 		64.3
(1.2)
P 		64.9
(1.3)
P 		52
B 111.4
(2)
P 		72.4
(-0.6)
P 		37.5
(-3)
P 		40
(-2.8)
P 		81.6
(0)
P 		125.4
(3)
P 		88
C 91.1
(1.9)
P 		85.6
(1.4)
P 		43.4
(-2.4)
P 		37.1
(-3)
P 		55.5
(-1.3)
P 		103.8
(3)
P 		67
avr 81.3
(1.4) 		79.3
(1.2) 		36.4
(-3) 		41.3
(-2.5) 		67.1
(-0) 		98
(3) 		62
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176778_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2113
Sequence Type Exemplar sequence
Representative Public ID M01G12.12 NCBI
Target Description M01G12.12 /REP_DB=WormBase Gene ID /WP=CE23872 /GB=CAC35914.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060656(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060656 NCBI Caenorhabditis elegans RNA-dependent RNA polymerase Family family member (rrf-2) (rrf-2) mRNA, complete cds. 11/11 None
M01G12.12 ENSEMBL cdna:known chromosome:CEL140:I:12106806:12114099:-1 gene:M01G12.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:12106768-12114030(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol rrf-2
Ensembl M01G12.12
Entrez Gene 173092 Entrez gene
SwissProt Q9BH56 EMBL-EBI
WormBase CE23872 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3968 RNA-directed RNA polymerase activity inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAC35915 Hypothetical protein M01G12.12 [Caenorhabditis elegans] emb|CAC35914.1| Hypothetical protein M01G12.12 [Caenorhabditis elegans] ref|NP_493057.1| RNA-dependent RNA polymerase Family family member (rrf-2) [Caenorhabditis elegans] 0.0
blast CAC42290 Hypothetical protein F26A3.8 [Caenorhabditis elegans] ref|NP_492131.1| RNA-dependent RNA polymerase Family family member (rrf-1) [Caenorhabditis elegans] gb|AAF80368.1| RRF-1 [Caenorhabditis elegans] 0.0
blast CAE72312 Hypothetical protein CBG19448 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR007855 EMBL-EBI RNA dependent RNA polymerase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:176778_S_AT
gagccagatattccgcttactaggcatcagcggtcggacatgatgttcaagttcttccaa
ctcatgggaagtcgaaagttccgaacaatgcggtccatctccttccgcccacttggtttt
tcgtccgtcttcatgagtggcgagtggatggtattccatgagccggctctaaagacatac
tataatattatgttcaatctacgattcgaagagcttccaatatcaactgatccgacaacc
acacatgagacaagactccgagagtgtgagccatttgtgattgagttaccagaaaaagca
aatatggatatgctgatcaaaaaactaacagaaaaatcaaaagtctctcaattgaacttg
cgtcggcaagacaaaaacaagaagacgtcaaactctacagttcgctacattgtctctgcc
tcgggaacatttgaatcaactcagcagctcagaaaactcgtaaccgtcacgagtccaatc
aaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCCAGATATTCCGCTTACTAGGC 407 385 4192 Antisense
CTTACTAGGCATCAGCGGTCGGACA 428 221 4207 Antisense
GTTCCGAACAATGCGGTCCATCTCC 639 439 4269 Antisense
TTTTTCGTCCGTCTTCATGAGTGGC 246 673 4308 Antisense
TGGATGGTATTCCATGAGCCGGCTC 154 551 4336 Antisense
ATATCAACTGATCCGACAACCACAC 502 19 4411 Antisense
GTCTCTCAATTGAACTTGCGTCGGC 555 469 4534 Antisense
GAAGACGTCAAACTCTACAGTTCGC 288 339 4572 Antisense
TACAGTTCGCTACATTGTCTCTGCC 63 645 4587 Antisense
GCAGCTCAGAAAACTCGTAACCGTC 394 313 4635 Antisense
GTAACCGTCACGAGTCCAATCAAAA 139 463 4651 Antisense
Sequence Source Affymetrix Proprietary Database