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[176771_at]

Description Y50D7A.5 /REP_DB=WormBase Gene ID /WP=CE26142 /GB=AAK68512.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 03      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 398
1 2 3 4 range
A 118.5
(-3)
P 		141.3
(-2.6)
A 		117.6
(-3)
A 		475
(3)
P 		357
B 132.2
(-2.5)
P 		129.6
(-2.5)
M 		107.5
(-3)
P 		389.6
(3)
P 		282
C 125.9
(-2)
P 		101.4
(-2.5)
A 		77.5
(-3)
A 		358.6
(3)
P 		281
avr 125.5
(-2.5) 		124.1
(-2.5) 		100.9
(-3) 		407.7
(3) 		307

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 205
1 2 3 4 5 6 range
A 90.2
(-2.3)
A 		122.2
(-1)
A 		72.6
(-3)
A 		114
(-1.3)
A 		132.6
(-0.6)
P 		221
(3)
P 		148
B 98.3
(-3)
P 		111.2
(-1.8)
A 		126
(-0.4)
P 		132.3
(0.2)
P 		154.8
(2.3)
P 		162.5
(3)
P 		64
C 90.6
(-0.3)
A 		79.1
(-0.7)
M 		110.8
(0.5)
A 		16
(-3)
A 		179.4
(3)
P 		123.6
(1)
P 		163
avr 93
(-2.6) 		104.2
(-1.8) 		103.1
(-1.8) 		87.4
(-3) 		155.6
(2) 		169
(3) 		82
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 58
1 2 3 4 range
A 43.2
(-1.1)
P 		30.7
(-3)
A 		30.7
(-3)
A 		69.5
(3)
P 		39
B 42.2
(-1.2)
P 		32
(-2.3)
M 		24.9
(-3)
P 		83
(3)
P 		58
C 41.7
(-0.4)
P 		29.1
(-2.6)
A 		27
(-3)
A 		60.5
(3)
P 		34
avr 42.4
(-0.9) 		30.6
(-2.6) 		27.5
(-3) 		71
(3) 		44

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 60
1 2 3 4 5 6 range
A 16.6
(-2.9)
A 		43.5
(1.4)
A 		15.8
(-3)
A 		28.9
(-0.9)
A 		40
(0.8)
P 		53.8
(3)
P 		38
B 34.2
(-2.3)
P 		37
(-1.8)
A 		30.6
(-3)
P 		35.1
(-2.2)
P 		62.9
(3)
P 		60.6
(2.6)
P 		32
C 33.6
(0.3)
A 		24.1
(-0.7)
M 		28.2
(-0.3)
A 		3.2
(-3)
A 		58.1
(3)
P 		41.4
(1.2)
P 		55
avr 28.1
(-1.9) 		34.9
(-0.6) 		24.9
(-2.5) 		22.4
(-3) 		53.7
(3) 		51.9
(2.7) 		31
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176771_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8248
Sequence Type Exemplar sequence
Representative Public ID Y50D7A.5 NCBI
Target Description Y50D7A.5 /REP_DB=WormBase Gene ID /WP=CE26142 /GB=AAK68512.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064778(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064778 NCBI Caenorhabditis elegans Y50D7A.5 (Y50D7A.5) mRNA, complete cds. 11/11 A 
GENEFINDER00000021197 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:221418:235694:1 11/11 None
Y50D7A.5 ENSEMBL cdna:known chromosome:CEL140:III:230434:235051:1 gene:Y50D7A.5 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000021185 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:230433-235032(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y50D7A.5
Entrez Gene 175188 Entrez gene
SwissProt Q965T5 EMBL-EBI
WormBase CE26142 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68513 Kinesin-like protein protein 20 [Caenorhabditis elegans] ref|NP_497178.1| Kinesin-Like Protein family member (klp-20) [Caenorhabditis elegans] 0.0
blast AAF99087 KRP85 [Caenorhabditis elegans] 0.0
blast CAE69512 Hypothetical protein CBG15720 [Caenorhabditis briggsae] 0.0
blast AAK68512 Hypothetical protein Y50D7A.5 [Caenorhabditis elegans] ref|NP_497179.1| Y50D7A.5 [Caenorhabditis elegans] 9.0E-98
blast CAE69511 Hypothetical protein CBG15719 [Caenorhabditis briggsae] 4.0E-67
Protein Domains
Database ID Description E-Value
scop a.2.7.Methicillin resistance protein FemA probable tRNA-binding arm All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Methicillin resistance protein FemA probable tRNA-binding arm 2.59999990463257
Pfam IPR001752 EMBL-EBI Kinesin, motor region 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497179.1 1 5-27
Y50D7A.5 1 5-27
GENEFINDER00000021197 2 680-702,898-920
Sequence
Target Sequence 
>C. ELEGANS:176771_AT
cattcattgtcattgctgtgcttcttgccaccaccatcccgtctgccacgtcatctatcc
gtcgtttcattgtcaaaaatgaggttttcatgggcagacaggtccccgaattttgcatgg
actactttgagagctcgtctgccaagctaaagactgaaaatatcaaccgcgagctgatca
gcaacgcccttgacaacgagaacgactggcagaaacaggagcaaaatttcatcctacaag
ccacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CATTCATTGTCATTGCTGTGCTTCT 183 217 20 Antisense
GTCATCTATCCGTCGTTTCATTGTC 569 467 69 Antisense
GGTTTTCATGGGCAGACAGGTCCCC 348 503 102 Antisense
CAGACAGGTCCCCGAATTTTGCATG 113 205 114 Antisense
CCCCGAATTTTGCATGGACTACTTT 269 1 123 Antisense
GGACTACTTTGAGAGCTCGTCTGCC 7 525 138 Antisense
GAGCTCGTCTGCCAAGCTAAAGACT 436 387 150 Antisense
CAACCGCGAGCTGATCAGCAACGCC 507 187 183 Antisense
GCAACGCCCTTGACAACGAGAACGA 83 321 200 Antisense
GACAACGAGAACGACTGGCAGAAAC 446 365 211 Antisense
GCAAAATTTCATCCTACAAGCCACC 23 319 240 Antisense
Sequence Source Affymetrix Proprietary Database