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[176766_at]

Description Y46H3D.8 /REP_DB=WormBase Gene ID /WP=CE22016 /GB=AAK72311.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 29      4grp_total > 43      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 27
1 2 3 4 range
A 4.4
(-3)
A 		12.3
(3)
A 		11.4
(2.3)
A 		4.4
(-3)
A 		8
B 8.5
(-0.8)
A 		15.5
(3)
A 		6.5
(-1.9)
A 		4.4
(-3)
A 		11
C 3.5
(-3)
A 		30.7
(3)
A 		6.8
(-2.3)
A 		8.9
(-1.8)
A 		27
avr 5.5
(-3) 		19.5
(3) 		8.2
(-1.8) 		5.9
(-2.8) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 21
1 2 3 4 5 6 range
A 23.6
(3)
A 		3.3
(-2.7)
A 		3.9
(-2.5)
A 		2.3
(-3)
A 		8.1
(-1.4)
A 		3.2
(-2.7)
A 		21
B 4
(-2.6)
A 		4
(-2.6)
A 		5
(-1.7)
A 		3.6
(-3)
A 		10.2
(3)
A 		5.8
(-1)
A 		7
C 3.7
(-3)
A 		9.3
(2.7)
A 		7.8
(1.2)
A 		4.5
(-2.2)
A 		9.6
(3)
A 		6.9
(0.3)
A 		6
avr 10.4
(3) 		5.5
(-1.3) 		5.6
(-1.2) 		3.5
(-3) 		9.3
(2) 		5.3
(-1.4) 		7
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 1.6
(-0.5)
A 		2.7
(2.3)
A 		3
(3)
A 		0.6
(-3)
A 		2
B 2.7
(0.7)
A 		3.8
(3)
A 		1.5
(-1.8)
A 		0.9
(-3)
A 		3
C 1.2
(-3)
A 		8.8
(3)
A 		2.4
(-2.1)
A 		1.5
(-2.8)
A 		8
avr 1.8
(-1.8) 		5.1
(3) 		2.3
(-1.1) 		1
(-3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4
1 2 3 4 5 6 range
A 4.4
(3)
A 		1.2
(-2.1)
A 		0.8
(-2.7)
A 		0.6
(-3)
A 		2.4
(-0.2)
A 		0.8
(-2.7)
A 		4
B 1.4
(-2.3)
A 		1.3
(-2.4)
A 		1.2
(-2.6)
A 		1
(-3)
A 		4.2
(3)
A 		2.2
(-0.8)
A 		3
C 1.4
(-1.6)
A 		2.8
(2.2)
A 		2
(0)
A 		0.9
(-3)
A 		3.1
(3)
A 		2.3
(0.8)
A 		2
avr 2.4
(1) 		1.8
(-0.5) 		1.3
(-1.8) 		0.8
(-3) 		3.2
(3) 		1.8
(-0.5) 		2
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176766_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15498
Sequence Type Exemplar sequence
Representative Public ID Y46H3D.8 NCBI
Target Description Y46H3D.8 /REP_DB=WormBase Gene ID /WP=CE22016 /GB=AAK72311.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071061(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071061 NCBI Caenorhabditis elegans Y46H3D.8 (Y46H3D.8) mRNA, complete cds. 11/11 None
SNAP00000018927 ENSEMBL cdna:SNAP chromosome:CEL140:V:1630138:1631077:-1 11/11 None
GENEFINDER00000018932 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:1630138:1631077:-1 11/11 None
Y46H3D.8 ENSEMBL cdna:known chromosome:CEL140:V:1630138:1631077:-1 gene:Y46H3D.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1630137-1631077(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y46H3D.8
Entrez Gene 189972 Entrez gene
SwissProt Q966B6 EMBL-EBI
WormBase CE22016 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_503462 Y46H3D.8 [Caenorhabditis elegans] gb|AAK72311.1| Hypothetical protein Y46H3D.8 [Caenorhabditis elegans] 1.0E-112
blast CAE65520 Hypothetical protein CBG10495 [Caenorhabditis briggsae] 1.0E-101
blast CAE65521 Hypothetical protein CBG10496 [Caenorhabditis briggsae] 1.0E-101
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 9.3
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.9E-10
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 5.1E-12
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.4E-13
Sequence
Target Sequence 
>C. ELEGANS:176766_AT
gaacgtcatctctgatgcttcgtgtcaagttttgtatgctgccccagacaccctctaccc
agctgctggaaatgatgcggagagagcgtatgcttgctacaccacggccacggccactcc
agcggcgactgtaagtgatatggttactgctgcaatttccacgtgcccaaagacttgcgg
cttttgctgtgccaccagtgcatattcctgctctaatgttgcttacccacgtctcacgtg
ctcctccatcacagctgctcagtgtgcttcatccgtctggcgtaccatcattgccgaaga
ctgtccagccgcttgtggattctgtaacgacggaggatgtgtcgactctgttgtggcctg
cgccaacgacatctccatctgtaccaccgtcggactccaagacttcgtgaacacctactg
ccagaagacgtgctccagatgcgcatcttccaccacaactgcttcttcgtcttccagcag
ctgcacttcatacaccgctgactccagctctgcctgtgcatcatgggccgccaatggatt
ct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACGTCATCTCTGATGCTTCGTGT 136 349 147 Antisense
AGAGAGCGTATGCTTGCTACACCAC 543 69 227 Antisense
TGGTTACTGCTGCAATTTCCACGTG 674 549 287 Antisense
TATTCCTGCTCTAATGTTGCTTACC 685 663 349 Antisense
TCACAGCTGCTCAGTGTGCTTCATC 14 627 395 Antisense
CATCATTGCCGAAGACTGTCCAGCC 492 213 432 Antisense
GAGGATGTGTCGACTCTGTTGTGGC 631 403 479 Antisense
GGACTCCAAGACTTCGTGAACACCT 95 525 538 Antisense
AGAAGACGTGCTCCAGATGCGCATC 158 75 569 Antisense
GTCTTCCAGCAGCTGCACTTCATAC 279 469 615 Antisense
GCATCATGGGCCGCCAATGGATTCT 271 309 664 Antisense
Sequence Source Affymetrix Proprietary Database