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[176754_at]

Description C55B7.9 /REP_DB=WormBase Gene ID /WP=CE09020 /GB=AAK68280.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 59      6grp_high1_10_10 > 95      4grp_highest_8_8 > 23      4grp_high_8_8 > 13      6grp_highest_nonorm > 84      6grp_total > 75      4grp_total > 02      6grp_highest_sub > In      6grp_cgc > 67      6grp_high1_sub > In      6grp_cgc_sub > In      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 487
1 2 3 4 range
A 472.1
(-2.1)
P 		478.9
(-2)
P 		424.9
(-3)
P 		745.9
(3)
P 		321
B 403.8
(-3)
P 		431.7
(-2.6)
P 		414.2
(-2.9)
P 		857.3
(3)
P 		454
C 516.3
(-3)
P 		513.6
(-3)
P 		531.2
(-2.7)
P 		890.5
(3)
P 		377
avr 464.1
(-2.9) 		474.7
(-2.7) 		456.8
(-3) 		831.2
(3) 		374

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 440
1 2 3 4 5 6 range
A 512.4
(-2.4)
P 		484.9
(-3)
P 		768.6
(3)
P 		664.6
(0.8)
P 		613.3
(-0.3)
P 		643.1
(0.3)
P 		284
B 488.3
(-3)
P 		511.5
(-2.5)
P 		529.5
(-2.2)
P 		676.5
(0.7)
P 		715.5
(1.5)
P 		791
(3)
P 		303
C 445.8
(-3)
P 		519.9
(-2)
P 		531.5
(-1.8)
P 		722.8
(0.8)
P 		599
(-0.9)
P 		886.1
(3)
P 		440
avr 482.2
(-3) 		505.4
(-2.5) 		609.9
(-0.4) 		688
(1.2) 		642.6
(0.3) 		773.4
(3) 		291
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 89
1 2 3 4 range
A 171.9
(3)
P 		104
(-3)
P 		111.1
(-2.4)
P 		109.2
(-2.5)
P 		68
B 128.8
(-0.7)
P 		106.6
(-2.3)
P 		96
(-3)
P 		182.7
(3)
P 		87
C 170.9
(0.7)
P 		147.7
(-3)
P 		185.1
(3)
P 		150.1
(-2.6)
P 		37
avr 157.2
(3) 		119.4
(-3) 		130.7
(-1.2) 		147.3
(1.4) 		38

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 202
1 2 3 4 5 6 range
A 94.5
(-3)
P 		172.5
(2.2)
P 		167.4
(1.8)
P 		168.6
(1.9)
P 		185.1
(3)
P 		156.6
(1.1)
P 		91
B 169.9
(-1.5)
P 		170.2
(-1.5)
P 		128.6
(-3)
P 		179.2
(-1.2)
P 		290.7
(2.8)
P 		294.9
(3)
P 		166
C 165.2
(-1.9)
P 		158.6
(-2.1)
P 		135.3
(-3)
P 		145.4
(-2.6)
P 		193.8
(-0.8)
P 		296.6
(3)
P 		161
avr 143.2
(-3) 		167.1
(-1.6) 		143.8
(-3) 		164.4
(-1.8) 		223.2
(1.5) 		249.4
(3) 		106
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176754_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1716
Sequence Type Exemplar sequence
Representative Public ID C55B7.9 NCBI
Target Description C55B7.9 /REP_DB=WormBase Gene ID /WP=CE09020 /GB=AAK68280.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059468(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059468 NCBI Caenorhabditis elegans MeDiaTor family member (mdt-18) (mdt-18) mRNA, complete cds. 11/11 None
SNAP00000038299 ENSEMBL cdna:SNAP chromosome:CEL140:I:6501020:6501966:-1 11/11 None
GENEFINDER00000038312 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6501020:6501966:-1 11/11 None
C55B7.9.1 ENSEMBL cdna:known chromosome:CEL140:I:6500982:6502381:-1 gene:C55B7.9 11/11 None
C55B7.9.2 ENSEMBL cdna:known chromosome:CEL140:I:6501020:6501982:-1 gene:C55B7.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6500948-6501895(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol mdt-18
Ensembl C55B7.9
Entrez Gene 183844 Entrez gene
SwissProt Q966M5 EMBL-EBI
WormBase CE09020 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_491869 MeDiaTor family member (mdt-18) [Caenorhabditis elegans] gb|AAK68280.1| Mediator protein 18 [Caenorhabditis elegans] 1.0E-131
blast CAE67206 Hypothetical protein CBG12642 [Caenorhabditis briggsae] 1.0E-100
Sequence
Target Sequence 
>C. ELEGANS:176754_AT
aatgtcccagacttggaacgacgtttagccggcctttgtgatccgggatgtgaggaattc
ttcgagcatgagatgtcattcagcctacgatcttcgggtataatggatatcaaaaccgat
attaaattgcgaagaagattccgaacagaacatcgaattcaaaattattggcaattaaag
tacattggagttccggaaccagatcaaaaatgcccaactattgttcgaaaagaaatcagt
tcacttgtccattctcaggatatgatgacgtatgcgaaatccttgggtctccgcatggac
tatgaatatatgacacaaggaaaattgtggacaaaaggaaatataaagattctgcactca
atattgactagtacttctcgcgcaggaacatatgattcaaattcaatcaaatctgtttct
gattctgcgttagtggaaatcagtatctcattgcccgagtctgcagaatacatgccggct
gccaagtctcttcgtgattttgccgatcagctgatgccac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATGTCCCAGACTTGGAACGACGTT 126 163 124 Antisense
TTAGCCGGCCTTTGTGATCCGGGAT 452 681 148 Antisense
CATTCAGCCTACGATCTTCGGGTAT 123 217 200 Antisense
AAATCAGTTCACTTGTCCATTCTCA 639 117 356 Antisense
CGTATGCGAAATCCTTGGGTCTCCG 100 247 392 Antisense
AATATTGACTAGTACTTCTCGCGCA 120 175 483 Antisense
TCTGTTTCTGATTCTGCGTTAGTGG 139 609 535 Antisense
TATCTCATTGCCCGAGTCTGCAGAA 72 659 567 Antisense
AGAATACATGCCGGCTGCCAAGTCT 460 75 588 Antisense
TGCCAAGTCTCTTCGTGATTTTGCC 268 587 603 Antisense
GATTTTGCCGATCAGCTGATGCCAC 572 427 619 Antisense
Sequence Source Affymetrix Proprietary Database