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[176752_at]

Description ZK177.4 /REP_DB=WormBase Gene ID /WP=CE26396 /GB=AAG00052.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 68      6grp_high1_10_10 > 96      4grp_highest_8_8 > 29      4grp_high_8_8 > 07      6grp_highest_nonorm > 83      6grp_total > 86      4grp_total > 0      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 148
1 2 3 4 range
A 347.3
(0.5)
P 		359.4
(3)
P 		330.1
(-3)
P 		346.4
(0.3)
P 		29
B 286.5
(-3)
P 		294.2
(-2.6)
P 		307.9
(-1.9)
P 		408.3
(3)
P 		122
C 327.6
(0.7)
P 		260.5
(-3)
P 		367.2
(2.9)
P 		369.6
(3)
P 		109
avr 320.5
(-1.6) 		304.7
(-3) 		335.1
(-0.4) 		374.8
(3) 		70

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 388
1 2 3 4 5 6 range
A 426
(-0.5)
P 		331.3
(-2.9)
P 		327.4
(-3)
P 		481.6
(0.9)
P 		434.4
(-0.3)
P 		563.2
(3)
P 		236
B 326.4
(-3)
P 		386.3
(-2)
P 		394.2
(-1.9)
P 		431.5
(-1.3)
P 		521.2
(0.2)
P 		693.7
(3)
P 		367
C 305.5
(-3)
P 		373.2
(-1.9)
P 		440.5
(-0.8)
P 		510.4
(0.3)
P 		427.3
(-1)
P 		673.7
(3)
P 		368
avr 352.6
(-3) 		363.6
(-2.8) 		387.4
(-2.3) 		474.5
(-0.5) 		461
(-0.8) 		643.5
(3) 		291
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 77
1 2 3 4 range
A 126.4
(3)
P 		78
(-0.8)
P 		86.3
(-0.2)
P 		50.7
(-3)
P 		76
B 91.4
(3)
P 		72.6
(-2.6)
P 		71.3
(-3)
P 		87
(1.7)
P 		20
C 108.4
(1.2)
P 		74.9
(-1.8)
P 		128
(3)
P 		62.3
(-3)
P 		66
avr 108.7
(3) 		75.2
(-1.8) 		95.2
(1.1) 		66.7
(-3) 		42

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 187
1 2 3 4 5 6 range
A 78.5
(-2.3)
P 		117.8
(1.2)
P 		71.3
(-3)
P 		122.1
(1.6)
P 		131.1
(2.5)
P 		137.1
(3)
P 		66
B 113.6
(-2.3)
P 		128.6
(-1.8)
P 		95.7
(-3)
P 		114.3
(-2.3)
P 		211.7
(1.3)
P 		258.7
(3)
P 		163
C 113.2
(-2.5)
P 		113.9
(-2.4)
P 		112.1
(-2.5)
P 		102.6
(-3)
P 		138.3
(-1.3)
P 		225.5
(3)
P 		123
avr 101.8
(-2.5) 		120.1
(-1.6) 		93
(-3) 		113
(-1.9) 		160.4
(0.5) 		207.1
(3) 		114
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176752_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4080
Sequence Type Exemplar sequence
Representative Public ID ZK177.4 NCBI
Target Description ZK177.4 /REP_DB=WormBase Gene ID /WP=CE26396 /GB=AAG00052.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062652(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062652 NCBI Caenorhabditis elegans ZK177.4a (ZK177.4) mRNA, complete cds. 11/11 None
SNAP00000012884 ENSEMBL cdna:SNAP chromosome:CEL140:II:5504017:5508945:1 11/11 None
GENEFINDER00000012894 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5506212:5508945:1 11/11 None
ZK177.4a.2 ENSEMBL cdna:known chromosome:CEL140:II:5506062:5508977:1 gene:ZK177.4 11/11 None
ZK177.4a.3 ENSEMBL cdna:known chromosome:CEL140:II:5506209:5508977:1 gene:ZK177.4 11/11 None
ZK177.4a.1 ENSEMBL cdna:known chromosome:CEL140:II:5506210:5508977:1 gene:ZK177.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5506209-5508943(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK177.4
Entrez Gene 3564830 Entrez gene
SwissProt Q09372 EMBL-EBI
WormBase CE29069 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAG00052 Hypothetical protein ZK177.4 [Caenorhabditis elegans] ref|NP_495053.1| ZK177.4a [Caenorhabditis elegans] sp|Q09372|YS44_CAEEL Hypothetical protein ZK177.4 in chromosome II 0.0
blast T27764 hypothetical protein ZK177.4 - Caenorhabditis elegans 0.0
blast CAE59124 Hypothetical protein CBG02419 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
p450 Q13120 Animalia; CYP2A6V2; hum; Q13120; Homo sapiens; Q13120 4.0E-27
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 2.0E-43
Sequence
Target Sequence 
>C. ELEGANS:176752_AT
gtacgggctctttggagcagcaacttgctgctttgaaaccacttgcagcttgcgtaatct
ccgaaaaagtatcatgcgaagaggcaattgatgccgctatgaagtttttcgatgcagttg
atgaaagattgaagaagcattgtgaaatctcaaagttattcgtggagagatgtctcgcag
tctcttcaagtgctcaagctcttgatgaaaagcaacaaagtgtggatcaacgactgattg
aggaaacaaacactgtcgaagaattgaaaattcgaatgcacgaaacaaaagaaagaatgg
aaggtgcgcgaaaaggaatcaatgtgctattccaccgggttgacgaaaatgtcccattaa
gtgacaatgaaattcgaattttcgagcgtctcaaggaacaccagaaaatgctctcggata
tgacaaaactagttccgaagatgacattagattcgaatgagatccaccgaatggccaata
tagtgctgaaaaaacgtggaacaggcgaagagcagaacagatttgcagctgttgagaaaa
atgctaccgaaatcgaaagcttggaagctcgcgagaacaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTACGGGCTCTTTGGAGCAGCAACT 365 459 1259 Antisense
GAAACCACTTGCAGCTTGCGTAATC 525 359 1293 Antisense
TGCAGCTTGCGTAATCTCCGAAAAA 225 577 1302 Antisense
GATGCCGCTATGAAGTTTTTCGATG 372 415 1348 Antisense
GGAGAGATGTCTCGCAGTCTCTTCA 286 519 1422 Antisense
GCAGTCTCTTCAAGTGCTCAAGCTC 663 311 1435 Antisense
TGTGCTATTCCACCGGGTTGACGAA 399 557 1581 Antisense
TTCGAGCGTCTCAAGGAACACCAGA 169 695 1639 Antisense
GGAACACCAGAAAATGCTCTCGGAT 239 529 1653 Antisense
GAATGAGATCCACCGAATGGCCAAT 317 331 1713 Antisense
AAGCTTGGAAGCTCGCGAGAACAAA 523 151 1815 Antisense
Sequence Source Affymetrix Proprietary Database