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[176744_s_at]

Description F54F2.5 /REP_DB=WormBase Gene ID /WP=CE25004 /GB=AAK26138.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 79      6grp_high1_10_10 > 97      4grp_high_8_8 > 21      6grp_highest_nonorm > 91      6grp_total > 97      4grp_total > 10      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 169
1 2 3 4 range
A 119.6
(-2)
P 		100.3
(-3)
A 		123.9
(-1.8)
A 		218.5
(3)
P 		118
B 115.3
(-3)
P 		158
(0.5)
P 		137.5
(-1.2)
M 		187.6
(3)
P 		72
C 129.1
(-2.4)
P 		154.6
(-1.5)
P 		114.6
(-3)
P 		269.6
(3)
P 		155
avr 121.3
(-3) 		137.6
(-2.1) 		125.3
(-2.8) 		225.2
(3) 		104

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 302
1 2 3 4 5 6 range
A 159
(-2.4)
P 		126.7
(-3)
P 		193
(-1.7)
P 		222.9
(-1.1)
P 		330.1
(1)
P 		428.3
(3)
P 		302
B 195
(-1.6)
P 		166.5
(-3)
P 		215.1
(-0.7)
P 		250.9
(1)
P 		291.7
(2.9)
P 		292.9
(3)
P 		126
C 127.5
(-3)
P 		240.7
(-0.1)
P 		173.4
(-1.8)
P 		197.6
(-1.2)
P 		252.9
(0.2)
P 		361.4
(3)
P 		234
avr 160.5
(-3) 		178
(-2.5) 		193.8
(-2) 		223.8
(-1.1) 		291.6
(0.9) 		360.9
(3) 		200
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 43.5
(3)
P 		21.8
(-3)
A 		32.4
(-0.1)
A 		32
(-0.2)
P 		22
B 36.8
(0.6)
P 		39
(2.3)
P 		31.9
(-3)
M 		40
(3)
P 		8
C 42.7
(0)
P 		44.4
(1.8)
P 		39.9
(-3)
P 		45.5
(3)
P 		6
avr 41
(3) 		35.1
(-2.6) 		34.7
(-3) 		39.2
(1.3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 92
1 2 3 4 5 6 range
A 29.3
(-3)
P 		45
(-1.7)
P 		42
(-2)
P 		56.5
(-0.8)
P 		99.6
(2.6)
P 		104.3
(3)
P 		75
B 67.8
(-1.6)
P 		55.4
(-2.7)
P 		52.2
(-3)
P 		66.5
(-1.7)
P 		118.5
(3)
P 		109.2
(2.2)
P 		66
C 47.3
(-2.4)
P 		73.4
(-0.5)
P 		44.1
(-2.7)
P 		39.7
(-3)
P 		81.8
(0.1)
P 		121
(3)
P 		81
avr 48.1
(-2.8) 		57.9
(-1.9) 		46.1
(-3) 		54.2
(-2.3) 		100
(1.9) 		111.5
(3) 		65
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176744_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6596
Sequence Type Exemplar sequence
Representative Public ID F54F2.5 NCBI
Target Description F54F2.5 /REP_DB=WormBase Gene ID /WP=CE25004 /GB=AAK26138.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066541(11), NM_181933(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066541 NCBI Caenorhabditis elegans F54F2.5a (F54F2.5) mRNA, complete cds. 11/11 None
NM_181933 NCBI Caenorhabditis elegans F54F2.5b (F54F2.5) mRNA, complete cds. 11/11 None
GENEFINDER00000044294 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8787456:8792240:-1 9/11 None
F54F2.5a ENSEMBL cdna:known chromosome:CEL140:III:8787038:8792240:-1 gene:F54F2.5 11/11 None
F54F2.5b ENSEMBL cdna:known chromosome:CEL140:III:8787168:8790020:-1 gene:F54F2.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000044283 6/11 Cross Hyb Matching Probes None
SNAP00000044284 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8787163-8792236(-) 100.0 100.0
Public Domain and Genome References
Ensembl F54F2.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK26138 Hypothetical protein F54F2.5a [Caenorhabditis elegans] ref|NP_498942.1| F54F2.5a [Caenorhabditis elegans] sp|P34450|YMA5_CAEEL Hypothetical zinc finger protein F54F2.5 in chromosome III 0.0
blast AAN63392 Hypothetical protein F54F2.5b [Caenorhabditis elegans] ref|NP_871662.1| F54F2.5b [Caenorhabditis elegans] 1.0E-126
blast AAK26138 Hypothetical protein F54F2.5a [Caenorhabditis elegans] ref|NP_498942.1| F54F2.5a [Caenorhabditis elegans] sp|P34450|YMA5_CAEEL Hypothetical zinc finger protein F54F2.5 in chromosome III 1.0E-126
blast AAN63392 Hypothetical protein F54F2.5b [Caenorhabditis elegans] ref|NP_871662.1| F54F2.5b [Caenorhabditis elegans] 1.0E-125
blast CAE62735 Hypothetical protein CBG06897 [Caenorhabditis briggsae] 1.0E-105
Sequence
Target Sequence 
>C. ELEGANS:176744_S_AT
ccacgttccggtaagcgaaccagtgatgatgatttaatgataacaaataagaaacgagaa
cgattggaaatggcaccaaatcttcatttccatcaagatattattcaccgacttccgaca
tcaacaacaactcaacgacaaaataacgccttccaaaacgaataccttcgtccgccagtg
tcctacggttatgatccacttattggaagatatccagatttccaactgatgtcccaatct
tcacttccatcctcatctgctagttcgagttcctatgacccatttgcaactgcagtttat
caagattcggatcgcaatccaatgcaaccgaatgatcaacacatttgtgatatgaataat
gttggggcatatgaaactgctaattttggtttggacaactcatcggatcttgtcgtcaac
gaagcaacaattgcatcaaatctaatggtgaatgaggttgaagatgaagtttttgaggag
ctgaataaactggactttgtcatgccagccgat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCACGTTCCGGTAAGCGAACCAGTG 247 271 1189 Antisense
TGGCACCAAATCTTCATTTCCATCA 43 547 1259 Antisense
AGATATTATTCACCGACTTCCGACA 589 63 1284 Antisense
GCCAGTGTCCTACGGTTATGATCCA 554 279 1362 Antisense
TTCCAACTGATGTCCCAATCTTCAC 402 701 1408 Antisense
TCCATCCTCATCTGCTAGTTCGAGT 349 591 1434 Antisense
TGCTAGTTCGAGTTCCTATGACCCA 43 579 1446 Antisense
CCTATGACCCATTTGCAACTGCAGT 675 267 1460 Antisense
CGGATCGCAATCCAATGCAACCGAA 654 243 1496 Antisense
AACTCATCGGATCTTGTCGTCAACG 282 137 1585 Antisense
ACTGGACTTTGTCATGCCAGCCGAT 118 97 1677 Antisense
Sequence Source Affymetrix Proprietary Database