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[176740_at]

Description M04F3.2 /REP_DB=WormBase Gene ID /WP=CE23875 /GB=AAK71381.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 44      4grp_total > 14      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 66
1 2 3 4 range
A 12.4
(-0.3)
A 		5.3
(-3)
A 		6
(-2.7)
A 		21.3
(3)
A 		16
B 43.8
(3)
A 		10.6
(-2.9)
A 		10.2
(-3)
A 		20
(-1.3)
A 		34
C 34.7
(-0.1)
A 		11.7
(-2.2)
A 		2.6
(-3)
A 		68.5
(3)
A 		66
avr 30.3
(1.8) 		9.2
(-2.4) 		6.3
(-3) 		36.6
(3) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 66
1 2 3 4 5 6 range
A 52.3
(1.7)
A 		24.7
(-1.2)
A 		64.6
(3)
A 		32.5
(-0.4)
A 		8.2
(-3)
A 		38.9
(0.3)
A 		56
B 70.9
(3)
A 		13.1
(-2.2)
A 		35.9
(-0.2)
A 		4.6
(-3)
A 		43.4
(0.5)
A 		49.3
(1)
A 		66
C 12.8
(-3)
A 		68
(3)
A 		56.4
(1.7)
A 		49.8
(1)
A 		35.2
(-0.6)
A 		18.4
(-2.4)
A 		55
avr 45.3
(1.2) 		35.3
(-1.4) 		52.3
(3) 		29
(-3) 		28.9
(-3) 		35.5
(-1.3) 		23
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 4.5
(3)
A 		1.1
(-3)
A 		1.6
(-2.1)
A 		3.1
(0.5)
A 		3
B 14
(3)
A 		2.6
(-2.9)
A 		2.4
(-3)
A 		4.3
(-2)
A 		12
C 11.5
(3)
A 		3.4
(-1.6)
A 		0.9
(-3)
A 		11.5
(3)
A 		11
avr 10
(3) 		2.4
(-2.4) 		1.6
(-3) 		6.3
(0.4) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 9.6
(0.7)
A 		8.8
(0.3)
A 		14.1
(3)
A 		8.2
(-0.1)
A 		2.5
(-3)
A 		9.5
(0.6)
A 		12
B 24.7
(3)
A 		4.4
(-2.2)
A 		8.7
(-1.1)
A 		1.2
(-3)
A 		17.6
(1.2)
A 		18.4
(1.4)
A 		24
C 4.8
(-3)
A 		20.7
(3)
A 		14.3
(0.6)
A 		10
(-1)
A 		11.4
(-0.5)
A 		6.2
(-2.5)
A 		16
avr 13
(3) 		11.3
(1.4) 		12.4
(2.4) 		6.5
(-3) 		10.5
(0.7) 		11.4
(1.5) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176740_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1526
Sequence Type Exemplar sequence
Representative Public ID M04F3.2 NCBI
Target Description M04F3.2 /REP_DB=WormBase Gene ID /WP=CE23875 /GB=AAK71381.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059044(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059044 NCBI Caenorhabditis elegans M04F3.2 (M04F3.2) mRNA, complete cds. 11/11 None
M04F3.2 ENSEMBL cdna:known chromosome:CEL140:I:4765755:4769402:1 gene:M04F3.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001029121 4/11 Cross Hyb Matching Probes None
GENEFINDER00000000883 8/11 Cross Hyb Matching Probes None
SNAP00000000877 8/11 Cross Hyb Matching Probes None
GENEFINDER00000017384 3/11 Cross Hyb Matching Probes None
Y80D3A.11 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4765754-4769402(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl M04F3.2
Entrez Gene 187447 Entrez gene
SwissProt Q95Y98 EMBL-EBI
WormBase CE36357 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK71381 Hypothetical protein M04F3.2 [Caenorhabditis elegans] ref|NP_491445.2| M04F3.2 [Caenorhabditis elegans] 1.0E-112
blast CAE18005 Hypothetical protein Y80D3A.11 [Caenorhabditis elegans] ref|NP_001024292.1| Y80D3A.11 [Caenorhabditis elegans] 1.0E-31
blast NP_491108 LiPid Depleted family member (lpd-6) [Caenorhabditis elegans] gb|AAB97575.2| Lipid depleted protein 6 [Caenorhabditis elegans] 8.0E-27
Sequence
Target Sequence 
>C. ELEGANS:176740_AT
gaatgctactcgtaagcaaggtgctactactggacaagttgaagaagttgaaaatcagac
ggagaaggatagagagattgtgatgaatcgggagagaaatctgaagcgtgccaacgagga
atggggaacaactgaagcctcgaagcgtccaagatatgaggatagtcgtggtgttttccg
aggtggattcagaggaagaagagaagctcgtggtggcttccgtagaagtgacgaccgagg
aggattcagaggacgtgacggaggtggtggcttccgtggtgggcaagacccgaagaactc
gggggatgtccaactgggggattcccaactcgggggattggccacgcccacagaaccgtg
ccttacaatacgcccatttctgcaacctccgcacgataaggataatattacatttaatga
attacataatttcaacaaaaaaaacaataatttaaaatcatcattcaaaaccgtgcggag
g

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATGCTACTCGTAAGCAAGGTGCT 127 331 189 Antisense
GCAAGGTGCTACTACTGGACAAGTT 663 321 204 Antisense
TCGGGAGAGAAATCTGAAGCGTGCC 514 605 276 Antisense
GAGGATAGTCGTGGTGTTTTCCGAG 69 403 346 Antisense
GTCGTGGTGTTTTCCGAGGTGGATT 75 473 353 Antisense
GGTGGCTTCCGTAGAAGTGACGACC 164 499 400 Antisense
GAAGTGACGACCGAGGAGGATTCAG 637 333 413 Antisense
AGGATTCAGAGGACGTGACGGAGGT 389 55 429 Antisense
CTTCCGTGGTGGGCAAGACCCGAAG 115 213 459 Antisense
TGCAACCTCCGCACGATAAGGATAA 539 575 569 Antisense
ATCATCATTCAAAACCGTGCGGAGG 453 29 645 Antisense
Sequence Source Affymetrix Proprietary Database