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[176738_s_at]

Description M04F3.5 /REP_DB=WormBase Gene ID /WP=CE12420 /GB=AAK71384.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 80      6grp_high1_10_10 > 19      6grp_highest_nonorm > 70      6grp_total > 29      4grp_total > 52      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 257
1 2 3 4 range
A 280.8
(3)
P 		277
(2.9)
P 		196.8
(0.7)
P 		65.7
(-3)
A 		215
B 249.1
(2.4)
P 		268
(3)
P 		262
(2.8)
P 		84.6
(-3)
A 		183
C 316.3
(2.8)
P 		323
(3)
P 		243.3
(0.8)
P 		105.7
(-3)
A 		217
avr 282.1
(2.8) 		289.3
(3) 		234
(1.4) 		85.3
(-3) 		204

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 289
1 2 3 4 5 6 range
A 400.1
(3)
P 		357.4
(2.1)
P 		111.6
(-3)
P 		267.7
(0.2)
P 		354.8
(2.1)
P 		210.8
(-0.9)
P 		289
B 358.1
(3)
P 		284
(-1.4)
P 		266.8
(-2.5)
P 		271.6
(-2.2)
P 		257.9
(-3)
P 		266.8
(-2.5)
P 		100
C 300
(-1)
P 		323.9
(2.5)
P 		286.6
(-3)
P 		317.6
(1.6)
P 		309.6
(0.4)
P 		327
(3)
P 		40
avr 352.7
(3) 		321.8
(1.6) 		221.7
(-3) 		285.6
(-0.1) 		307.4
(0.9) 		268.2
(-0.9) 		131
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 95
1 2 3 4 range
A 102.2
(3)
P 		60.1
(0.3)
P 		51.5
(-0.3)
P 		9.6
(-3)
A 		93
B 79.4
(3)
P 		66.2
(1.7)
P 		60.7
(1.2)
P 		18
(-3)
A 		61
C 104.7
(3)
P 		92.9
(2.2)
P 		84.8
(1.6)
P 		17.8
(-3)
A 		87
avr 95.4
(3) 		73.1
(1.3) 		65.7
(0.8) 		15.1
(-3) 		80

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 103
1 2 3 4 5 6 range
A 73.7
(-0.1)
P 		127.1
(3)
P 		24.3
(-3)
P 		67.9
(-0.5)
P 		107.1
(1.8)
P 		51.3
(-1.4)
P 		103
B 124.6
(3)
P 		94.5
(-0)
P 		64.8
(-3)
P 		71.9
(-2.3)
P 		104.8
(1)
P 		99.5
(0.5)
P 		60
C 111.2
(3)
P 		98.8
(1.4)
P 		72.9
(-1.9)
P 		63.9
(-3)
P 		100.2
(1.6)
P 		109.5
(2.8)
P 		47
avr 103.2
(2.6) 		106.8
(3) 		54
(-3) 		67.9
(-1.4) 		104
(2.7) 		86.8
(0.7) 		53
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176738_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1849
Sequence Type Exemplar sequence
Representative Public ID M04F3.5 NCBI
Target Description M04F3.5 /REP_DB=WormBase Gene ID /WP=CE12420 /GB=AAK71384.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059047(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059047 NCBI Caenorhabditis elegans M04F3.5 (M04F3.5) mRNA, complete cds. 11/11 None
M04F3.5 ENSEMBL cdna:known chromosome:CEL140:I:4773837:4777718:-1 gene:M04F3.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4774058-4776030(-) 99.21 100.0
Public Domain and Genome References
Gene Title
Ensembl M04F3.5
Entrez Gene 172093 Entrez gene
SwissProt Q95Y95 EMBL-EBI
WormBase CE31974 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.30862.1.S1_AT similar to CG33558-PA cfa
CANINE_2:CFAAFFX.30862.1.S1_S_AT similar to CG33558-PA cfa
CANINE_2:CFAAFFX.30863.1.S1_AT similar to CG33558-PA cfa
CANINE_2:CFAAFFX.30863.1.S1_X_AT similar to CG33558-PA cfa
DROSGENOME1:146704_AT dm
DROSOPHILA_2:1627329_AT dm
HG-U133_PLUS_2:213978_AT hypothetical protein BC002770 hs
HG-U133A_2:213978_AT hypothetical protein BC002770 hs
HG-U133A:213978_AT hypothetical protein BC002770 hs
HU35KSUBB:RC_AA024511_AT hypothetical protein BC002770 hs
U133_X3P:HS2.355835.2.S1_3P_S_AT hypothetical protein BC002770 hs
U133_X3P:HS.319825.1.A1_3P_AT hypothetical protein BC002770 hs
HG-U133_PLUS_2:1555894_S_AT hypothetical protein BC002770 hs
HG-U133_PLUS_2:1556175_AT hypothetical protein BC002770 hs
HG-U95AV2:33206_AT hypothetical protein BC002770 hs
MU11KSUBA:AA204106_S_AT cDNA sequence BC060632 mm
MG-U74AV2:94802_AT cDNA sequence BC060632 mm
MG-U74CV2:132102_AT cDNA sequence BC060632 mm
MOE430B:1435359_AT cDNA sequence BC060632 mm
MOUSE430_2:1435359_AT cDNA sequence BC060632 mm
RAE230B:1385315_AT Similar to actin monomer-binding protein (predicted) rn
RAT230_2:1385315_AT Similar to actin monomer-binding protein (predicted) rn
RAE230B:1396057_AT Similar to actin monomer-binding protein (predicted) rn
RAT230_2:1396057_AT Similar to actin monomer-binding protein (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAK71384 Hypothetical protein M04F3.5 [Caenorhabditis elegans] ref|NP_491448.2| M04F3.5 [Caenorhabditis elegans] 0.0
blast CAE60357 Hypothetical protein CBG03954 [Caenorhabditis briggsae] 0.0
blast NP_941027 hypothetical protein LOC244654 isoform 1 [Mus musculus] gb|AAH60632.1| Unknown (protein for MGC:79213) [Mus musculus] 3.0E-30
Sequence
Target Sequence 
>C. ELEGANS:176738_S_AT
gtctgcaagcgctttcttgatctctgaagcatgtcgagatattgatcaactgggaagtga
tctggagaactattgtagtgttcaagatcctacgagatactttattcatgaatcatctcc
atcgtcgggtcaaaagaatggaacaccgccagcagcaacgaacacaatgatagcagcaat
gattcaacgaagagcgtcacatgcaggagattatcagcaacaacaaacaatgtcagatcc
tggaaaacgggcttctgtttctacgttgggaagctctaatatgagtaattatggagtttt
atcacatcagccaccacttccacaatctgcaaattcgatgcaaaatcaacaagttcgcat
gagaactacatccgcatctcgaccaccaccaccagcccgccgtacatcccaaatcactgc
agccaccccgaccgctccttcagttgccgagcaacgaatgaatggatcaactatgggaag
ccgtcaatctctggattcggctggactttacgatccagcacatcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCTGCAAGCGCTTTCTTGATCTCT 572 471 279 Antisense
ATTCATGAATCATCTCCATCGTCGG 44 13 382 Antisense
CCGCCAGCAGCAACGAACACAATGA 314 261 424 Antisense
GAAGAGCGTCACATGCAGGAGATTA 389 339 467 Antisense
AGATCCTGGAAAACGGGCTTCTGTT 338 1 513 Antisense
GGGCTTCTGTTTCTACGTTGGGAAG 527 489 527 Antisense
TGGAGTTTTATCACATCAGCCACCA 638 549 570 Antisense
ACCACTTCCACAATCTGCAAATTCG 506 85 591 Antisense
GCTCCTTCAGTTGCCGAGCAACGAA 103 299 712 Antisense
AATCTCTGGATTCGGCTGGACTTTA 77 171 764 Antisense
TGGACTTTACGATCCAGCACATCCA 172 549 780 Antisense
Sequence Source Affymetrix Proprietary Database