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[176734_at]

Description F35H12.5 /REP_DB=WormBase Gene ID /WP=CE27155 /GB=AAK39231.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 20      6grp_total > 33      4grp_total > 59      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 152
1 2 3 4 range
A 168.1
(0.2)
P 		239.7
(3)
P 		187.7
(0.9)
M 		87.8
(-3)
A 		152
B 180.5
(3)
P 		167.3
(2)
P 		172.3
(2.4)
P 		100.3
(-3)
A 		80
C 166.8
(-0.3)
P 		190.9
(2.7)
P 		193.6
(3)
P 		144.9
(-3)
A 		49
avr 171.8
(1.1) 		199.3
(3) 		184.5
(2) 		111
(-3) 		88

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 134
1 2 3 4 5 6 range
A 250.6
(3)
P 		185.1
(0.1)
P 		232.9
(2.2)
P 		174.4
(-0.4)
P 		162
(-1)
P 		116.7
(-3)
M 		134
B 179.3
(0.7)
P 		197.2
(2.8)
P 		198.8
(3)
P 		193.8
(2.4)
P 		147.4
(-3)
P 		164.7
(-1)
P 		51
C 245.2
(3)
P 		157.6
(-1.5)
P 		228.9
(2.2)
P 		244.6
(3)
P 		128.1
(-3)
A 		144
(-2.2)
P 		117
avr 225
(3) 		180
(-0.2) 		220.2
(2.7) 		204.3
(1.5) 		145.8
(-2.7) 		141.8
(-3) 		83
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 55
1 2 3 4 range
A 61.2
(3)
P 		52
(1.9)
P 		49.1
(1.5)
M 		12.8
(-3)
A 		48
B 57.6
(3)
P 		41.3
(0.3)
P 		39.9
(0.1)
P 		21.4
(-3)
A 		36
C 55.2
(1.3)
P 		54.9
(1.2)
P 		67.5
(3)
P 		24.4
(-3)
A 		43
avr 58
(3) 		49.4
(1.7) 		52.2
(2.1) 		19.5
(-3) 		39

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 63
1 2 3 4 5 6 range
A 46.2
(-0.1)
P 		65.8
(3)
P 		50.7
(0.6)
P 		44.2
(-0.5)
P 		48.9
(0.3)
P 		28.4
(-3)
M 		37
B 62.4
(1.9)
P 		65.6
(3)
P 		48.3
(-3)
P 		51.3
(-2)
P 		59.9
(1)
P 		61.4
(1.5)
P 		17
C 90.9
(3)
P 		48.1
(-2.2)
P 		58.3
(-1)
P 		49.2
(-2.1)
P 		41.5
(-3)
A 		48.2
(-2.2)
P 		49
avr 66.5
(3) 		59.8
(1) 		52.4
(-1.1) 		48.2
(-2.4) 		50.1
(-1.8) 		46
(-3) 		21
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176734_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18354
Sequence Type Exemplar sequence
Representative Public ID F35H12.5 NCBI
Target Description F35H12.5 /REP_DB=WormBase Gene ID /WP=CE27155 /GB=AAK39231.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029461(11), NM_001029460(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029461 NCBI Caenorhabditis elegans F35H12.5b (F35H12.5) mRNA, complete cds. 11/11 None
NM_001029460 NCBI Caenorhabditis elegans F35H12.5a (F35H12.5) mRNA, complete cds. 11/11 None
SNAP00000028608 ENSEMBL cdna:SNAP chromosome:CEL140:X:915872:920069:1 11/11 None
GENEFINDER00000028614 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:915872:920069:1 10/11 None
F35H12.5b ENSEMBL cdna:known chromosome:CEL140:X:918351:920069:1 gene:F35H12.5 11/11 None
F35H12.5a ENSEMBL cdna:known chromosome:CEL140:X:918395:920069:1 gene:F35H12.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:918322-920069(+) 100.0 100.0
Public Domain and Genome References
Ensembl F35H12.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK39231 Hypothetical protein F35H12.5a [Caenorhabditis elegans] ref|NP_001024631.1| F35H12.5a [Caenorhabditis elegans] sp|Q8WT51|YL4M_CAEEL Hypothetical protein F35H12.5 in chromosome X 0.0
blast E89453 protein F35H12.3 [imported] - Caenorhabditis elegans 0.0
blast AAK39230 Suppressor/enhancer of lin-12 protein 12 [Caenorhabditis elegans] ref|NP_508175.1| Suppressor/Enhancer of Lin-12 family member (sel-12) [Caenorhabditis elegans] gb|AAD50991.1| presenilin SEL-12 [Caenorhabditis elegans] sp|P52166|PSN_CAEEL Presenilin sel-12 0.0
blast AAA85511 membrane protein 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR010463 EMBL-EBI Protein of unknown function DUF1057 1.0E-126
Pfam IPR010463 EMBL-EBI Protein of unknown function DUF1057 1.0E-126
Pfam IPR010463 EMBL-EBI Protein of unknown function DUF1057 1.0E-126
Pfam IPR001108 EMBL-EBI Peptidase A22A, presenilin 1.0E-126
Pfam IPR010463 EMBL-EBI Protein of unknown function DUF1057 5.1E-113
Pfam IPR010463 EMBL-EBI Protein of unknown function DUF1057 6.4E-80
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000028608 9 53-75,101-123,130-152,162-184,193-210,215-237,250-272,381-403,408-430
GENEFINDER00000028614 9 53-75,101-123,130-152,162-184,193-210,215-237,250-272,381-403,408-430
Sequence
Target Sequence 
>C. ELEGANS:176734_AT
gaggcggagaaaatgcactgcaacttacaagtatgttatcgaatgatgagaactggccac
tggttggggcagtgatgatcaactcgccagggttcgctccgcataaaggaatcagtaaaa
gaatgggcactataaacttcattatatctttgatcaaacgtcacaacaaaaccatcaact
caatccttcatccaattttgcattatttctacaacaatctcatcggtttgcgtgtttctc
atggaaaagtagcagccgctgcaattctgcctatgcaaacttttgcctttgacgagcaaa
aactttcaattgacgatctacgtgcaaaacctggaattcgcgcgttctatggttacggat
ctaaggattttttaattgatgagcatcaatcggaagaagttgcgatgtacttttctgaag
aagatcattatgtaatttccaacaaacaagacgcggaaaaagctattaaagaagccagga
aaagctttacaactggaaaacaatttgtcacagcaaacttcaaagaagaaggtcactttc
tgcagaagacatatcccgagtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGGCGGAGAAAATGCACTGCAACT 35 405 590 Antisense
GAGAACTGGCCACTGGTTGGGGCAG 547 391 637 Antisense
TTGGGGCAGTGATGATCAACTCGCC 92 709 653 Antisense
CAGGGTTCGCTCCGCATAAAGGAAT 625 205 677 Antisense
CAAAACCATCAACTCAATCCTTCAT 204 191 756 Antisense
AATCTCATCGGTTTGCGTGTTTCTC 371 169 805 Antisense
AGTAGCAGCCGCTGCAATTCTGCCT 319 63 837 Antisense
AATTCTGCCTATGCAAACTTTTGCC 484 177 852 Antisense
GCAAAACCTGGAATTCGCGCGTTCT 548 317 913 Antisense
TCGCGCGTTCTATGGTTACGGATCT 136 599 927 Antisense
TTCTGCAGAAGACATATCCCGAGTT 441 685 1127 Antisense
Sequence Source Affymetrix Proprietary Database