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[176731_at]

Description Y54H5A.2 /REP_DB=WormBase Gene ID /WP=CE27293 /GB=AAK29945.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 78      4grp_highest_8_8 > 30      4grp_high_8_8 > 05      6grp_total > 69      4grp_total > 02      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 883
1 2 3 4 range
A 200.6
(-2.6)
P 		176.8
(-2.8)
P 		161.8
(-3)
P 		744.8
(3)
P 		583
B 204.6
(-2.3)
P 		124.2
(-3)
P 		194.4
(-2.4)
P 		848.8
(3)
P 		725
C 322.5
(-1.7)
P 		125.7
(-3)
P 		202.1
(-2.5)
P 		1007.1
(3)
P 		881
avr 242.6
(-2.2) 		142.2
(-3) 		186.1
(-2.6) 		866.9
(3) 		725

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 175
1 2 3 4 5 6 range
A 136.7
(-1.5)
P 		132.5
(-1.7)
P 		97.3
(-3)
P 		164.6
(-0.4)
P 		138.8
(-1.4)
P 		254.6
(3)
P 		157
B 89.8
(-2.5)
P 		138.9
(0.4)
P 		81.8
(-3)
P 		159.2
(1.6)
P 		154.9
(1.3)
P 		183
(3)
P 		101
C 159.6
(2.7)
P 		96.9
(-1.8)
P 		86
(-2.5)
A 		79.4
(-3)
P 		150.6
(2)
P 		164
(3)
P 		85
avr 128.7
(-0.8) 		122.8
(-1.2) 		88.4
(-3) 		134.4
(-0.5) 		148.1
(0.2) 		200.5
(3) 		112
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 150
1 2 3 4 range
A 73
(-0.1)
P 		38.4
(-3)
P 		42.3
(-2.7)
P 		109
(3)
P 		71
B 65.2
(-1.6)
P 		30.7
(-3)
P 		45.1
(-2.4)
P 		180.9
(3)
P 		150
C 106.8
(0.2)
P 		36.1
(-3)
P 		70.4
(-1.5)
P 		169.8
(3)
P 		134
avr 81.7
(-0.6) 		35.1
(-3) 		52.6
(-2.1) 		153.2
(3) 		118

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 52
1 2 3 4 5 6 range
A 25.2
(-2.4)
P 		47.1
(0.8)
P 		21.2
(-3)
P 		41.7
(0)
P 		41.9
(0)
P 		62
(3)
P 		41
B 31.3
(-1.6)
P 		46.2
(0.3)
P 		19.9
(-3)
P 		42.2
(-0.2)
P 		62.9
(2.3)
P 		68.2
(3)
P 		48
C 59.2
(3)
P 		29.6
(-1.1)
P 		21.9
(-2.2)
A 		16
(-3)
P 		48.7
(1.5)
P 		54.9
(2.4)
P 		43
avr 38.6
(-0.4) 		41
(-0.1) 		21
(-3) 		33.3
(-1.2) 		51.2
(1.5) 		61.7
(3) 		41
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176731_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7263
Sequence Type Exemplar sequence
Representative Public ID Y54H5A.2 NCBI
Target Description Y54H5A.2 /REP_DB=WormBase Gene ID /WP=CE27293 /GB=AAK29945.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065688
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
SNAP00000038482 ENSEMBL cdna:SNAP chromosome:CEL140:III:5148366:5149535:-1 None
GENEFINDER00000038488 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5145868:5149535:-1 None
Y54H5A.2.2 ENSEMBL cdna:known chromosome:CEL140:III:5137279:5149539:-1 gene:Y54H5A.2 None
Y54H5A.2.1 ENSEMBL cdna:known chromosome:CEL140:III:5137279:5149535:-1 gene:Y54H5A.2 None
NM_065688 NCBI Caenorhabditis elegans Y54H5A.2 (Y54H5A.2) mRNA, complete cds. A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5137407-5149534(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y54H5A.2
Entrez Gene 175699 Entrez gene
SwissProt Q9BL64 EMBL-EBI
WormBase CE32024 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK29945 Hypothetical protein Y54H5A.2 [Caenorhabditis elegans] ref|NP_498089.2| Y54H5A.2 [Caenorhabditis elegans] 0.0
blast AAK29945 Hypothetical protein Y54H5A.2 [Caenorhabditis elegans] ref|NP_498089.2| Y54H5A.2 [Caenorhabditis elegans] 1.0E-123
blast AAK29945 Hypothetical protein Y54H5A.2 [Caenorhabditis elegans] ref|NP_498089.2| Y54H5A.2 [Caenorhabditis elegans] 3.0E-74
blast CAE73770 Hypothetical protein CBG21314 [Caenorhabditis briggsae] 7.0E-69
blast CAE73770 Hypothetical protein CBG21314 [Caenorhabditis briggsae] 2.0E-68
blast CAE73770 Hypothetical protein CBG21314 [Caenorhabditis briggsae] 3.0E-68
Protein Domains
Database ID Description E-Value
Pfam IPR007205 EMBL-EBI Protein of unknown function DUF383 4.5E-113
Pfam IPR007206 EMBL-EBI Protein of unknown function DUF384 6.1E-26
Pfam IPR007205 EMBL-EBI Protein of unknown function DUF383 3.2E-76
Pfam IPR007205 EMBL-EBI Protein of unknown function DUF383 9.6E-27
Sequence
Target Sequence 
>C. ELEGANS:176731_AT
gaactgaaactctggcagctctcacaaacttcacttctggttccattgaagccgctgatt
ttgtattggataaatcaaaatgtgtggaaattgcttatactgcagttgttactaatgcct
tgtattcgtcagttgcctctcgtctgcttgtcaatgtttcccgccatttcccagatcgtg
ttgatcataagttgaaagcacgaaatgcgaattatattggacaactgctcagtgagtttc
ttttggtgaattcaaaatatacttcaaaaattgaattaaaaatgttttttgagtttcaaa
aattctttctagaaactacggttcgtgtggtaaaaacaaatcctcattcaattctcgaaa
ctacagtaactgcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACTGAAACTCTGGCAGCTCTCAC 114 349 215 Antisense
CTCACAAACTTCACTTCTGGTTCCA 223 227 235 Antisense
TCTGGTTCCATTGAAGCCGCTGATT 637 605 250 Antisense
ACTGCAGTTGTTACTAATGCCTTGT 148 99 313 Antisense
TAATGCCTTGTATTCGTCAGTTGCC 93 637 327 Antisense
GCCATTTCCCAGATCGTGTTGATCA 313 281 377 Antisense
GAATTATATTGGACAACTGCTCAGT 24 325 423 Antisense
CTGCTCAGTGAGTTTCTTTTGGTGA 464 237 439 Antisense
CTTTCTAGAAACTACGGTTCGTGTG 422 221 519 Antisense
AAACAAATCCTCATTCAATTCTCGA 12 129 548 Antisense
AATTCTCGAAACTACAGTAACTGCC 333 175 564 Antisense
Sequence Source Affymetrix Proprietary Database