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[176727_at]

Description T28F2.7 /REP_DB=WormBase Gene ID /WP=CE26013 /GB=AAB53057.2 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 87      4grp_total > 10      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 109
1 2 3 4 range
A 124.7
(1.6)
P 		105
(0.4)
A 		48.6
(-3)
A 		147.2
(3)
P 		99
B 92.2
(-3)
A 		123.5
(3)
A 		112.6
(0.9)
A 		117.4
(1.8)
P 		31
C 101.8
(-3)
P 		102.4
(-2.9)
A 		158
(3)
P 		126
(-0.4)
P 		56
avr 106.2
(-3) 		110.3
(-2) 		106.4
(-3) 		130.2
(3) 		24

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 215
1 2 3 4 5 6 range
A 79.4
(-1.4)
A 		52.9
(-3)
A 		87.1
(-1)
P 		72.9
(-1.8)
P 		135
(1.8)
A 		154.7
(3)
P 		102
B 115.1
(-0.7)
A 		66.1
(-3)
A 		136.2
(0.2)
A 		114.3
(-0.8)
A 		117.8
(-0.6)
P 		196.5
(3)
P 		130
C 59.3
(-2.6)
A 		44.3
(-3)
A 		103.5
(-1.3)
A 		90.7
(-1.7)
A 		118.9
(-0.9)
M 		259
(3)
P 		215
avr 84.6
(-1.8) 		54.4
(-3) 		108.9
(-0.8) 		92.6
(-1.5) 		123.9
(-0.2) 		203.4
(3) 		149
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 42
1 2 3 4 range
A 45.4
(3)
P 		22.8
(-1.1)
A 		12.7
(-3)
A 		21.5
(-1.4)
P 		33
B 29.4
(1.8)
A 		30.5
(3)
A 		26.1
(-1.8)
A 		25
(-3)
P 		6
C 33.7
(-0.8)
P 		29.4
(-1.6)
A 		55.1
(3)
P 		21.3
(-3)
P 		34
avr 36.2
(3) 		27.6
(-0.8) 		31.3
(0.8) 		22.6
(-3) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 73
1 2 3 4 5 6 range
A 14.6
(-3)
A 		18.8
(-2)
A 		19
(-2)
P 		18.5
(-2.1)
P 		40.7
(3)
A 		37.7
(2.3)
P 		26
B 40
(-0.9)
A 		22
(-3)
A 		33.1
(-1.7)
A 		30.3
(-2)
A 		47.9
(0)
P 		73.3
(3)
P 		51
C 22
(-2.3)
A 		13.5
(-3)
A 		26.4
(-1.9)
A 		18.2
(-2.6)
A 		38.5
(-1)
M 		86.7
(3)
P 		73
avr 25.5
(-2.1) 		18.1
(-3) 		26.2
(-2) 		22.3
(-2.5) 		42.4
(0.1) 		65.9
(3) 		48
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176727_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.252
Sequence Type Exemplar sequence
Representative Public ID T28F2.7 NCBI
Target Description T28F2.7 /REP_DB=WormBase Gene ID /WP=CE26013 /GB=AAB53057.2 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058795(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058795 NCBI Caenorhabditis elegans T28F2.7 (T28F2.7) mRNA, complete cds. 11/11 None
T28F2.7 ENSEMBL cdna:known chromosome:CEL140:I:3655634:3660979:-1 gene:T28F2.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3655633-3660979(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T28F2.7
Entrez Gene 189051 Entrez gene
SwissProt O01664 EMBL-EBI
WormBase CE26013 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.8002.1.S1_AT similar to amiloride-sensitive cation channel 3 isoform a cfa
CANINE_2:CFAAFFX.8011.1.S1_AT similar to amiloride-sensitive cation channel 3 isoform a cfa
CANINE_2:CFAAFFX.8014.1.S1_S_AT similar to amiloride-sensitive cation channel 3 isoform a cfa
HU35KSUBD:RC_AA448259_AT amiloride-sensitive cation channel 3 hs
HG-U133A:207561_S_AT amiloride-sensitive cation channel 3 hs
HG-U133_PLUS_2:207561_S_AT amiloride-sensitive cation channel 3 hs
HG-FOCUS:207561_S_AT amiloride-sensitive cation channel 3 hs
HG-U133A_2:207561_S_AT amiloride-sensitive cation channel 3 hs
U133_X3P:G9998947_3P_A_AT amiloride-sensitive cation channel 3 hs
HG-U95AV2:41079_AT amiloride-sensitive cation channel 3 hs
RG-U34A:RC_AI179632_AT amiloride-sensitive cation channel 3 rn
RAE230A:1370839_AT amiloride-sensitive cation channel 3 rn
RAT230_2:1370839_AT amiloride-sensitive cation channel 3 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6814 sodium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5272 sodium channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB53057 Hypothetical protein T28F2.7 [Caenorhabditis elegans] ref|NP_491196.1| T28F2.7 [Caenorhabditis elegans] 0.0
blast CAE66646 Hypothetical protein CBG11983 [Caenorhabditis briggsae] 0.0
blast CAE66721 Hypothetical protein CBG12067 [Caenorhabditis briggsae] 1.0E-135
Protein Domains
Database ID Description E-Value
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 3.3E-100
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491196.1 1 488-510
T28F2.7 1 488-510
Sequence
Target Sequence 
>C. ELEGANS:176727_AT
gagacttggacgattccgcatatgaaattaaacttccaagttgtgaacgtcttcttccgt
gacatgtcctacacagaatacattcaaaagcgtggaatgtcactcaccgaactgctctcc
gatatcggtggtaatatgggaatgttcatgggaatgtccgtgtttactattatcgaactc
ttcctatttttatcaaagatcggatggatcggattttcgagaaagcgaagagattatatg
tattcgaagaagaaaaatgaggaaatgcatgagaaagagctggaagatgttgtcactgga
ttcaagttgtttaggcatcgaaaatctggaaaagatatgagtcatttgagagaaaagatt
aaaggattatcaatgcacagagtaacatcggaacagttgaacgtttgtaaactcgcatgg
gaaaacgagccagacatcgagcggagactagcatcagttacaagacaaaactctgcgctc
aaggagcacaaagactacaaacagccaacaattttgccgtttgatctgaaagatatcaag
gatcaaataacacgtggaagggctgcatcaatgtttcgaagtcggc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGACTTGGACGATTCCGCATATGA 668 391 1324 Antisense
AACTTCCAAGTTGTGAACGTCTTCT 653 93 1354 Antisense
TTCTTCCGTGACATGTCCTACACAG 677 689 1375 Antisense
AAGCGTGGAATGTCACTCACCGAAC 459 149 1411 Antisense
CCGAACTGCTCTCCGATATCGGTGG 593 261 1430 Antisense
GGGAATGTCCGTGTTTACTATTATC 627 221 1473 Antisense
TTATCGAACTCTTCCTATTTTTATC 288 681 1493 Antisense
GAGCCAGACATCGAGCGGAGACTAG 314 385 1750 Antisense
GACAAAACTCTGCGCTCAAGGAGCA 372 365 1787 Antisense
AACAGCCAACAATTTTGCCGTTTGA 382 135 1823 Antisense
GCTGCATCAATGTTTCGAAGTCGGC 293 297 1885 Antisense
Sequence Source Affymetrix Proprietary Database