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[176718_at]

Description Y37E11C.C /REP_DB=WormBase Gene ID /WP=CE26069 /CHR=4 /FEA=Sanger Annotation /DEF=locus:unc-33 (ST.LOUIS)
Cluster 6grp_total > 19      4grp_total > 07      6grp_cgc > 01      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 8.2
(-3)
A 		9.9
(-1.9)
A 		13.5
(0.3)
A 		17.9
(3)
A 		10
B 26.3
(3)
A 		9.5
(-2.3)
A 		14.5
(-0.7)
A 		7.1
(-3)
A 		19
C 8.6
(-3)
A 		13.7
(1.6)
A 		11.6
(-0.3)
A 		15.3
(3)
A 		7
avr 14.4
(3) 		11
(-3) 		13.2
(0.9) 		13.4
(1.2) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 24.3
(3)
A 		4.6
(-2.5)
A 		11.3
(-0.7)
A 		5.7
(-2.2)
A 		14.1
(0.1)
A 		3
(-3)
A 		21
B 4.2
(-2.9)
A 		7.2
(-1.1)
A 		4.1
(-3)
A 		13.6
(2.8)
A 		13.9
(3)
A 		9.4
(0.2)
A 		10
C 11.6
(3)
A 		7.2
(0.1)
A 		5.1
(-1.2)
A 		8.2
(0.8)
A 		4.3
(-1.8)
A 		2.4
(-3)
A 		9
avr 13.4
(3) 		6.3
(-2) 		6.8
(-1.7) 		9.2
(0) 		10.8
(1.2) 		4.9
(-3) 		9
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7
1 2 3 4 range
A 3
(0.9)
A 		2.1
(-3)
A 		3.5
(3)
A 		2.6
(-0.9)
A 		1
B 8.4
(3)
A 		2.3
(-2.3)
A 		3.4
(-1.3)
A 		1.5
(-3)
A 		7
C 2.8
(-2.1)
A 		4
(3)
A 		4
(3)
A 		2.6
(-3)
A 		1
avr 4.7
(3) 		2.8
(-1.6) 		3.6
(0.4) 		2.2
(-3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5
1 2 3 4 5 6 range
A 4.5
(3)
A 		1.6
(-1.6)
A 		2.5
(-0.2)
A 		1.5
(-1.7)
A 		4.2
(2.5)
A 		0.7
(-3)
A 		4
B 1.5
(-2.3)
A 		2.4
(-1.2)
A 		1
(-3)
A 		3.6
(0.4)
A 		5.6
(3)
A 		3.5
(0.3)
A 		5
C 4.3
(3)
A 		2.2
(-0.6)
A 		1.3
(-2.1)
A 		1.6
(-1.6)
A 		1.4
(-2)
A 		0.8
(-3)
A 		4
avr 3.4
(2.1) 		2.1
(-1.6) 		1.6
(-3) 		2.2
(-1.3) 		3.7
(3) 		1.7
(-2.7) 		2
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176718_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19363
Sequence Type Exemplar sequence
Representative Public ID Y37E11C.C NCBI
Target Description Y37E11C.C /REP_DB=WormBase Gene ID /WP=CE26069 /CHR=4 /FEA=Sanger Annotation /DEF=locus:unc-33 (ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171303(11), NM_171304(11), NM_171305(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171303 NCBI Caenorhabditis elegans UNCoordinated family member (unc-33) (unc-33) mRNA, complete cds. 11/11 A 
NM_171304 NCBI Caenorhabditis elegans UNCoordinated family member (unc-33) (unc-33) mRNA, complete cds. 11/11 None
NM_171305 NCBI Caenorhabditis elegans UNCoordinated family member (unc-33) (unc-33) mRNA, complete cds. 11/11 None
SNAP00000011889 ENSEMBL cdna:SNAP chromosome:CEL140:IV:3522479:3525568:1 11/11 None
GENEFINDER00000011896 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:3519309:3525568:1 11/11 None
Y37E11C.1a ENSEMBL cdna:known chromosome:CEL140:IV:3516818:3526598:1 gene:Y37E11C.1 11/11 A 
Y37E11C.1b ENSEMBL cdna:known chromosome:CEL140:IV:3518144:3525568:1 gene:Y37E11C.1 11/11 A 
Y37E11C.1c ENSEMBL cdna:known chromosome:CEL140:IV:3519681:3525568:1 gene:Y37E11C.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:3521106-3525574(+) 98.68 100.0
Public Domain and Genome References
Gene Title
Gene Symbol unc-33
Ensembl Y37E11C.1
Entrez Gene 177112 Entrez gene
SwissProt Q01630 EMBL-EBI
WormBase CE21557 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
16787 hydrolase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA78520 hypothetical polypeptide I [Caenorhabditis elegans] emb|CAA78516.1| unc-33 [Caenorhabditis elegans] ref|NP_741357.1| UNCoordinated family member (unc-33) [Caenorhabditis elegans] gb|AAM97965.1| Uncoordinated protein 33 [Caenorhabditis elegans] sp|Q01630|UNC33_CAEEL Uncoordinated protein 33 (Protein unc-33) 0.0
blast CAA78521 hypothetical polypeptide II [Caenorhabditis elegans] emb|CAA78517.1| hypothetical [Caenorhabditis elegans] ref|NP_741358.1| UNCoordinated family member (unc-33) [Caenorhabditis elegans] 0.0
blast CAA78522 hypothetical polypeptide III [Caenorhabditis elegans] emb|CAA78518.1| hypothetical [Caenorhabditis elegans] ref|NP_741359.1| UNCoordinated family member (unc-33) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006680 EMBL-EBI Amidohydrolase 1.9E-11
Pfam IPR006680 EMBL-EBI Amidohydrolase 1.9E-11
Pfam IPR006680 EMBL-EBI Amidohydrolase 1.9E-11
Pfam IPR006680 EMBL-EBI Amidohydrolase 1.9E-11
Pfam IPR006680 EMBL-EBI Amidohydrolase 1.9E-11
Sequence
Target Sequence 
>C. ELEGANS:176718_AT
gaatcgatgcgatgcgtttcgtggcggtcacctcaacgaacgctgcgaaaatgttcaata
tgtacccgaaaaagggccgaattgcagtcggcgccgacgctgatctcgtaatttgggatg
ccagtggaaagcgagtcctcgagtcgagccgagcacagagctcacaggagaattcgatgt
acgacgggctcactgtgcattcggtggtgacggcgacgattgttggcggaaaaattgcct
atcagaatggagaagttcgggaggcaccggtagccggaggttttctgcgtctctcgccaa
attctccctatctcttcagtatggttgggcagagggataagttcgccaacgtggagcgtg
tcgaacgagaagcctcctcacaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATCGATGCGATGCGTTTCGTGGC 575 329 1067 Antisense
CGCCGACGCTGATCTCGTAATTTGG 154 257 1158 Antisense
GGAAAGCGAGTCCTCGAGTCGAGCC 676 527 1192 Antisense
GAGTCGAGCCGAGCACAGAGCTCAC 600 399 1207 Antisense
ATTCGATGTACGACGGGCTCACTGT 625 7 1238 Antisense
GGCTCACTGTGCATTCGGTGGTGAC 626 537 1253 Antisense
GTGACGGCGACGATTGTTGGCGGAA 568 481 1273 Antisense
CACCGGTAGCCGGAGGTTTTCTGCG 603 199 1331 Antisense
TCTCCCTATCTCTTCAGTATGGTTG 299 611 1369 Antisense
GTTCGCCAACGTGGAGCGTGTCGAA 287 439 1407 Antisense
TGTCGAACGAGAAGCCTCCTCACAA 541 557 1425 Antisense
Sequence Source Affymetrix Proprietary Database