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[176704_at]

Description Y66A7A.5 /REP_DB=WormBase Gene ID /WP=CE18545 /GB=CAC35859.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 97      4grp_total > 23      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 72
1 2 3 4 range
A 69.8
(3)
A 		18.1
(-2.8)
A 		16
(-3)
A 		23.6
(-2.2)
A 		54
B 19.5
(-1.8)
A 		8.8
(-3)
A 		9.8
(-2.9)
A 		64.2
(3)
A 		55
C 77.3
(3)
A 		9.4
(-2.7)
A 		5.7
(-3)
A 		60.8
(1.6)
A 		72
avr 55.5
(3) 		12.1
(-2.8) 		10.5
(-3) 		49.5
(2.2) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 220
1 2 3 4 5 6 range
A 20.9
(-2.6)
A 		16.2
(-2.7)
A 		7.5
(-3)
A 		104.3
(-0.1)
A 		162.3
(1.6)
A 		210.6
(3)
A 		203
B 41.8
(-1.8)
A 		13.4
(-3)
A 		65.8
(-0.8)
A 		84.3
(-0.1)
A 		93.7
(0.3)
A 		158.4
(3)
A 		145
C 49
(-2)
A 		41.1
(-2.2)
A 		14.2
(-3)
A 		19.3
(-2.9)
A 		128.3
(0.2)
A 		227.1
(3)
A 		213
avr 37.2
(-2.5) 		23.6
(-3) 		29.2
(-2.8) 		69.3
(-1.4) 		128.1
(0.6) 		198.7
(3) 		175
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 25.4
(3)
A 		3.9
(-2.9)
A 		4.2
(-2.8)
A 		3.5
(-3)
A 		22
B 6.2
(-0.9)
A 		2.2
(-3)
A 		2.3
(-2.9)
A 		13.7
(3)
A 		12
C 25.6
(3)
A 		2.7
(-2.8)
A 		2
(-3)
A 		10.2
(-0.9)
A 		24
avr 19.1
(3) 		2.9
(-3) 		2.8
(-3) 		9.1
(-0.7) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 74
1 2 3 4 5 6 range
A 3.8
(-2.7)
A 		5.8
(-2.5)
A 		1.6
(-3)
A 		26.5
(0)
A 		49
(2.7)
A 		51.3
(3)
A 		50
B 14.5
(-1.9)
A 		4.5
(-3)
A 		16
(-1.7)
A 		22.3
(-1)
A 		38.1
(0.7)
A 		59.1
(3)
A 		55
C 18.2
(-1.8)
A 		12.5
(-2.3)
A 		3.6
(-3)
A 		3.9
(-3)
A 		41.5
(0.1)
A 		76
(3)
A 		72
avr 12.2
(-2.4) 		7.6
(-2.9) 		7.1
(-3) 		17.6
(-1.9) 		42.9
(0.9) 		62.1
(3) 		55
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176704_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6628
Sequence Type Exemplar sequence
Representative Public ID Y66A7A.5 NCBI
Target Description Y66A7A.5 /REP_DB=WormBase Gene ID /WP=CE18545 /GB=CAC35859.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067102(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067102 NCBI Caenorhabditis elegans Y66A7A.5 (Y66A7A.5) mRNA, complete cds. 10/11 None
SNAP00000036268 ENSEMBL cdna:SNAP chromosome:CEL140:III:11612483:11623767:1 10/11 None
GENEFINDER00000036272 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:11616635:11623767:1 11/11 None
Y66A7A.5 ENSEMBL cdna:known chromosome:CEL140:III:11616635:11623845:1 gene:Y66A7A.5 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:11616630-11623763(+) 99.86 100.0
Public Domain and Genome References
Gene Title
Ensembl Y66A7A.5
Entrez Gene 176594 Entrez gene
SwissProt Q7K6J1 EMBL-EBI
WormBase CE34229 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
3700 transcription factor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAC35859 Hypothetical protein Y66A7A.5 [Caenorhabditis elegans] ref|NP_499503.2| Y66A7A.5 [Caenorhabditis elegans] 0.0
blast CAA21499 Y66A7A.5 [Caenorhabditis elegans] 0.0
blast CAC35858 Hypothetical protein Y66A7A.4 [Caenorhabditis elegans] ref|NP_499502.1| Y66A7A.4 [Caenorhabditis elegans] emb|CAB63400.1| Y66A7A.4 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
scop a.4.1.Homeodomain All alpha proteins; DNA/RNA-binding 3-helical bundle; Homeodomain-like; Homeodomain 0.0560000017285347
scop a.4.1.Homeodomain All alpha proteins; DNA/RNA-binding 3-helical bundle; Homeodomain-like; Homeodomain 0.0299999993294477
scop a.4.1.Homeodomain All alpha proteins; DNA/RNA-binding 3-helical bundle; Homeodomain-like; Homeodomain 0.061999998986721
Sequence
Target Sequence 
>C. ELEGANS:176704_AT
aaaaacatccaaattccacatctaatacatcagatttcaccggaacaattttgcagataa
tagatcaaacgtggagaacattttctgtaaacgacgaaaaacggctccattcgacatttc
ttcaaaatccgcaaccaacagatgagcaatattgttatttggcgaaagaactacgtgctc
cagtgaaagaagtgatgaattggttcaaattgaaacaaatgaacgaggaacgcgaggaag
aagcgctgaaattcgaatataagcttgatgcgctcacacaggaaagcatcgatttgttca
cgacaattgttgatggaactactgtagtctcggtcgccaagcgacgattgctagcactag
agcttgacgttccatacgaaacactcggtcgatggatcttctactaccgaaatcgcgagg
aaaaaagccggaaatctccgaaactcgaggatgatgatgatccaccgccgaggctggcga
aaatgccaaggcttgagatgaatgagcccgacaacgaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAAACATCCAAATTCCACATCTAA 104 131 1679 Antisense
GAAAAACGGCTCCATTCGACATTTC 312 355 1774 Antisense
CATTTCTTCAAAATCCGCAACCAAC 541 215 1793 Antisense
TAAGCTTGATGCGCTCACACAGGAA 395 637 1938 Antisense
GTAGTCTCGGTCGCCAAGCGACGAT 64 119 2002 Antisense
AGCACTAGAGCTTGACGTTCCATAC 445 77 2031 Antisense
GACGTTCCATACGAAACACTCGGTC 232 377 2044 Antisense
AACACTCGGTCGATGGATCTTCTAC 33 137 2058 Antisense
GGATCTTCTACTACCGAAATCGCGA 531 509 2072 Antisense
ATCCACCGCCGAGGCTGGCGAAAAT 564 39 2138 Antisense
GAGATGAATGAGCCCGACAACGAAG 356 395 2173 Antisense
Sequence Source Affymetrix Proprietary Database