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[176702_at]

Description C25F6.3 /REP_DB=WormBase Gene ID /WP=CE02496 /GB=AAK39195.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 32      6grp_high1_10_10 > 13      4grp_highest_8_8 > 25      4grp_high_8_8 > 60      6grp_highest_nonorm > 12      6grp_total > 24      4grp_total > 60      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3786
1 2 3 4 range
A 3562.2
(1.3)
P 		4351
(2.6)
P 		4592.2
(3)
P 		1014.4
(-3)
P 		3578
B 4121.2
(2)
P 		4071.7
(2)
P 		4710.3
(3)
P 		1019.8
(-3)
P 		3691
C 3768.9
(1.8)
P 		4104.6
(2.3)
P 		4503
(3)
P 		924.2
(-3)
P 		3579
avr 3817.4
(1.7) 		4175.8
(2.3) 		4601.8
(3) 		986.1
(-3) 		3616

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2537
1 2 3 4 5 6 range
A 3156.7
(3)
P 		3009.6
(2.6)
P 		1908.1
(-0)
P 		1444.1
(-1.2)
P 		941.9
(-2.4)
P 		690.2
(-3)
P 		2467
B 2712.1
(2.3)
P 		2981
(3)
P 		2231.5
(1.1)
P 		1698.3
(-0.3)
P 		863.5
(-2.4)
P 		619.3
(-3)
P 		2362
C 2756.5
(2.8)
P 		2845.1
(3)
P 		2177.9
(1.2)
P 		1540.4
(-0.6)
P 		1015.7
(-2.1)
P 		677.9
(-3)
P 		2167
avr 2875.1
(2.8) 		2945.2
(3) 		2105.8
(0.8) 		1560.9
(-0.6) 		940.4
(-2.3) 		662.5
(-3) 		2283
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1421
1 2 3 4 range
A 1297
(3)
P 		944.5
(1.2)
P 		1200.5
(2.5)
P 		148.5
(-3)
P 		1149
B 1314.2
(3)
P 		1005.6
(1.3)
P 		1091.5
(1.8)
P 		217.3
(-3)
P 		1097
C 1247.6
(1.6)
P 		1180.1
(1.3)
P 		1569.4
(3)
P 		155.8
(-3)
P 		1414
avr 1286.3
(3) 		1043.4
(1.7) 		1287.1
(3) 		173.9
(-3) 		1113

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 902
1 2 3 4 5 6 range
A 581.9
(-0.2)
P 		1070.4
(3)
P 		415.5
(-1.4)
P 		366.3
(-1.7)
P 		284.2
(-2.2)
P 		168.1
(-3)
P 		902
B 943.7
(2.6)
P 		992.2
(3)
P 		541.9
(-0.5)
P 		449.8
(-1.3)
P 		350.8
(-2.1)
P 		230.9
(-3)
P 		761
C 1021.7
(3)
P 		868.1
(1.8)
P 		554.3
(-0.5)
P 		309.8
(-2.4)
P 		328.7
(-2.2)
P 		226.9
(-3)
P 		795
avr 849.1
(2) 		976.9
(3) 		503.9
(-0.7) 		375.3
(-1.7) 		321.2
(-2.1) 		208.6
(-3) 		768
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176702_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17110
Sequence Type Exemplar sequence
Representative Public ID C25F6.3 NCBI
Target Description C25F6.3 /REP_DB=WormBase Gene ID /WP=CE02496 /GB=AAK39195.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076526(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076526 NCBI Caenorhabditis elegans C25F6.3 (C25F6.3) mRNA, complete cds. 11/11 None
SNAP00000000865 ENSEMBL cdna:SNAP chromosome:CEL140:X:5467761:5472484:1 11/11 None
GENEFINDER00000000871 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:5467836:5472484:1 11/11 None
C25F6.3 ENSEMBL cdna:known chromosome:CEL140:X:5467761:5472632:1 gene:C25F6.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:5467761-5472485(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C25F6.3
Entrez Gene 180818 Entrez gene
WormBase CE38489 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:258162_AT dihydroorotate dehydrogenase family protein / dihydroorotate oxidase family protein at
CANINE_2:CFA.20077.1.S1_AT similar to Dihydropyrimidine dehydrogenase [NADP+] precursor (DPD) (DHPDHase) (Dihydrouracil dehydrogenase) (Dihydrothymine dehydrogenase) cfa
CANINE_2:CFAAFFX.30709.1.S1_S_AT similar to Dihydropyrimidine dehydrogenase [NADP+] precursor (DPD) (DHPDHase) (Dihydrouracil dehydrogenase) (Dihydrothymine dehydrogenase) cfa
CANINE_2:CFAAFFX.30711.1.S1_AT similar to Dihydropyrimidine dehydrogenase [NADP+] precursor (DPD) (DHPDHase) (Dihydrouracil dehydrogenase) (Dihydrothymine dehydrogenase) cfa
CANINE_2:CFAAFFX.30717.1.S1_AT similar to Dihydropyrimidine dehydrogenase [NADP+] precursor (DPD) (DHPDHase) (Dihydrouracil dehydrogenase) (Dihydrothymine dehydrogenase) cfa
DROSOPHILA_2:1625686_S_AT Rhythmically expressed gene 3 dm
DROSGENOME1:151805_AT Rhythmically expressed gene 3 dm
CHICKEN:GGAAFFX.3458.1.S1_S_AT dihydropyrimidine dehydrogenase gga
HG-U133_PLUS_2:204646_AT dihydropyrimidine dehydrogenase hs
HG-U133A:204646_AT dihydropyrimidine dehydrogenase hs
HG-FOCUS:204646_AT dihydropyrimidine dehydrogenase hs
HG-U133A_2:204646_AT dihydropyrimidine dehydrogenase hs
HUGENEFL:U20938_AT dihydropyrimidine dehydrogenase hs
HUGENEFL:U09178_S_AT dihydropyrimidine dehydrogenase hs
U133_X3P:G4557874_3P_AT dihydropyrimidine dehydrogenase hs
U133_X3P:HS2.1602.2.S2_3P_AT dihydropyrimidine dehydrogenase hs
U133_X3P:HS2.1602.2.S1_3P_AT dihydropyrimidine dehydrogenase hs
HG-U133_PLUS_2:1554534_AT dihydropyrimidine dehydrogenase hs
HG-U133_PLUS_2:1554536_AT dihydropyrimidine dehydrogenase hs
HG-U95AV2:38220_AT dihydropyrimidine dehydrogenase hs
U133_X3P:HS2.383624.1.S1_3P_X_AT SEC63-like (S. cerevisiae) hs
U133_X3P:1566966_3P_AT SEC63-like (S. cerevisiae) hs
HG-U133_PLUS_2:1566966_AT SEC63-like (S. cerevisiae) hs
HG-U133_PLUS_2:1566965_AT SEC63-like (S. cerevisiae) hs
HG-U95C:66093_AT SEC63-like (S. cerevisiae) hs
HU35KSUBC:RC_N74467_AT SEC63-like (S. cerevisiae) hs
HU35KSUBC:RC_W49559_AT SEC63-like (S. cerevisiae) hs
MOE430A:1427946_S_AT dihydropyrimidine dehydrogenase mm
MOUSE430A_2:1427946_S_AT dihydropyrimidine dehydrogenase mm
MOUSE430_2:1427946_S_AT dihydropyrimidine dehydrogenase mm
MOE430A:1427945_AT dihydropyrimidine dehydrogenase mm
MOUSE430A_2:1427945_AT dihydropyrimidine dehydrogenase mm
MOUSE430_2:1427945_AT dihydropyrimidine dehydrogenase mm
MG-U74BV2:111949_AT dihydropyrimidine dehydrogenase mm
MOE430B:1443717_AT dihydropyrimidine dehydrogenase mm
MOUSE430_2:1443717_AT dihydropyrimidine dehydrogenase mm
MG-U74CV2:134224_AT Dihydropyrimidine dehydrogenase, mRNA (cDNA clone MGC:47313 IMAGE:4235971) mm
MU19KSUBC:TC35038_AT Dihydropyrimidine dehydrogenase, mRNA (cDNA clone MGC:47313 IMAGE:4235971) mm
Protein Similarities
Method ID Description E-Value
blast AAK39195 Hypothetical protein C25F6.3 [Caenorhabditis elegans] ref|NP_508927.2| C25F6.3 [Caenorhabditis elegans] 0.0
blast Q18164 Probable dihydropyrimidine dehydrogenase [NADP+] (DPD) (DHPDHase) (Dihydrouracil dehydrogenase) (Dihydrothymine dehydrogenase) 0.0
blast CAE68756 Hypothetical protein CBG14689 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
scop a.1.2.Dihydropyrimidine dehydrogenase, N-terminal domain All alpha proteins; Globin-like; alpha-helical ferredoxin; Dihydropyrimidine dehydrogenase, N-terminal domain 0.0
Pfam IPR001450 EMBL-EBI 4Fe-4S ferredoxin, iron-sulfur binding domain 7.7E-5
Pfam IPR001295 EMBL-EBI Dihydroorotate dehydrogenase 1.2E-23
Pfam IPR001450 EMBL-EBI 4Fe-4S ferredoxin, iron-sulfur binding domain 7.7E-5
Pfam IPR001295 EMBL-EBI Dihydroorotate dehydrogenase 1.2E-23
Pfam IPR001450 EMBL-EBI 4Fe-4S ferredoxin, iron-sulfur binding domain 7.7E-5
Pfam IPR001295 EMBL-EBI Dihydroorotate dehydrogenase 1.2E-23
Trans Membrane
ID Number Of Domains Domain Boundaries
C25F6.3 1 5-27
Sequence
Target Sequence 
>C. ELEGANS:176702_AT
ctgggatggacaatcgccaccaatcgaaaaacatcaaaaaggaaaacctatcttgcttca
aggacaaaagaaaatgccattcttcggaaagtacagggatgaacgtgaaaagcttgaggc
tattaaactgtcagaaagcaaccttttggacaccgaaaactatcactttgcttcgagacc
agacactcaagtttcgcgtgtcccaactgttgaagatgtcattggaaaagccctgccacg
tattggcccatatgtcacactcgacaaccaagagcagaaggttgcaattattgatgatga
tatgtgcatcaattgtggaaagtgttatatgacatgcaatgattctggataccaagctat
cacttttgatccagtcacccatcagccacatgttacggaagacgattgtactggatgtac
tctttgctacagtgtgtgcccgattccggaatgtatagaaatggtgccaagaactgggcc
atggaaggctccaaaacgtggtgtgaagccatcggtcgagccaggaactccaaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTGGGATGGACAATCGCCACCAATC 177 239 2673 Antisense
TATCACTTTGCTTCGAGACCAGACA 601 657 2833 Antisense
GAGACCAGACACTCAAGTTTCGCGT 491 393 2847 Antisense
TTTCGCGTGTCCCAACTGTTGAAGA 622 669 2864 Antisense
GGAAAAGCCCTGCCACGTATTGGCC 57 503 2896 Antisense
TTGGCCCATATGTCACACTCGACAA 446 709 2915 Antisense
GCTATCACTTTTGATCCAGTCACCC 436 303 3028 Antisense
ACCCATCAGCCACATGTTACGGAAG 709 89 3049 Antisense
TACAGTGTGTGCCCGATTCCGGAAT 591 643 3100 Antisense
ACGTGGTGTGAAGCCATCGGTCGAG 336 95 3168 Antisense
ATCGGTCGAGCCAGGAACTCCAAAA 179 3 3183 Antisense
Sequence Source Affymetrix Proprietary Database