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[176701_at]

Description M04F3.1 /REP_DB=WormBase Gene ID /WP=CE12414 /GB=AAK71380.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 96      6grp_high1_10_10 > 69      4grp_highest_8_8 > 13      4grp_high_8_8 > 30      6grp_highest_nonorm > 78      6grp_total > 69      4grp_total > 24      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1148
1 2 3 4 range
A 1662.6
(-3)
P 		2016.3
(-1.1)
P 		2395.3
(0.9)
P 		2784.5
(3)
P 		1122
B 1799.7
(-3)
P 		2252.3
(0.9)
P 		2416.5
(2.3)
P 		2497
(3)
P 		697
C 1636.9
(-3)
P 		2310.5
(1)
P 		2148.6
(0)
P 		2649.4
(3)
P 		1013
avr 1699.7
(-3) 		2193
(0.1) 		2320.1
(0.9) 		2643.6
(3) 		944

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 934
1 2 3 4 5 6 range
A 1586.9
(0)
P 		1633.8
(0.6)
P 		1341.2
(-3)
P 		1660.9
(0.9)
P 		1506.1
(-1)
P 		1830
(3)
P 		489
B 1637.8
(1.7)
P 		1565.1
(1)
P 		1155.5
(-3)
P 		1451.9
(-0.1)
P 		1774
(3)
P 		1667.2
(2)
P 		619
C 1675.5
(-1.5)
P 		1649.8
(-1.8)
P 		1540.4
(-3)
P 		1653.2
(-1.8)
P 		1684.2
(-1.4)
P 		2089.9
(3)
P 		550
avr 1633.4
(0.3) 		1616.2
(0.1) 		1345.7
(-3) 		1588.7
(-0.2) 		1654.8
(0.6) 		1862.4
(3) 		517
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 341
1 2 3 4 range
A 605.4
(2.4)
P 		437.7
(-2.2)
P 		626.2
(3)
P 		407.6
(-3)
P 		219
B 573.9
(3)
P 		556.3
(0.5)
P 		560
(1)
P 		532.1
(-3)
P 		42
C 541.8
(-1.1)
P 		664.3
(1.3)
P 		748.9
(3)
P 		446.7
(-3)
P 		302
avr 573.7
(0.7) 		552.8
(-0) 		645
(3) 		462.1
(-3) 		183

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 440
1 2 3 4 5 6 range
A 292.5
(-3)
P 		581
(3)
P 		292.1
(-3)
P 		421.2
(-0.3)
P 		454.5
(0.4)
P 		445.6
(0.2)
P 		289
B 569.9
(0.9)
P 		521
(0.3)
P 		280.6
(-3)
P 		384.6
(-1.6)
P 		720.8
(3)
P 		621.7
(1.6)
P 		440
C 621
(1.7)
P 		503.4
(-0.2)
P 		392.1
(-2)
P 		332.5
(-3)
P 		545
(0.5)
P 		699.6
(3)
P 		367
avr 494.5
(0.9) 		535.1
(1.8) 		321.6
(-3) 		379.4
(-1.7) 		573.4
(2.6) 		589
(3) 		267
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176701_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1636
Sequence Type Exemplar sequence
Representative Public ID M04F3.1 NCBI
Target Description M04F3.1 /REP_DB=WormBase Gene ID /WP=CE12414 /GB=AAK71380.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059045(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059045 NCBI Caenorhabditis elegans M04F3.1 (M04F3.1) mRNA, complete cds. 11/11 None
SNAP00000000878 ENSEMBL cdna:SNAP chromosome:CEL140:I:4770305:4771267:1 11/11 None
GENEFINDER00000000886 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:4770305:4771267:1 11/11 None
M04F3.1.1 ENSEMBL cdna:known chromosome:CEL140:I:4770292:4771355:1 gene:M04F3.1 11/11 None
M04F3.1.2 ENSEMBL cdna:known chromosome:CEL140:I:4770293:4771349:1 gene:M04F3.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4770304-4771267(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl M04F3.1
Entrez Gene 172092 Entrez gene
SwissProt Q95Y97 EMBL-EBI
WormBase CE12414 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK71380 Hypothetical protein M04F3.1 [Caenorhabditis elegans] ref|NP_491446.1| M04F3.1 [Caenorhabditis elegans] 1.0E-163
blast CAE60359 Hypothetical protein CBG03956 [Caenorhabditis briggsae] 1.0E-111
Sequence
Target Sequence 
>C. ELEGANS:176701_AT
tacacgtacgacctctgtgatccaaacaacacggagatggaatatcgtacgctcaagtat
gacaatgagggttcgacattcgatcattcttcaattgtcgagggaactcgtgttcgagcc
atcggaaagctcaagggattcgatggaaacaataccattatgctgttcaacatcactcca
gttgaagacgacaaggatttcacgatcttcgagctcgaggcagaagctgcacgtctgttc
ttccaaaagaacgtgagcgagaaactgaagtcggtcgactcggatggcttccaaggaatg
ctggcaccaccgaagagcagaatgtcacaaacatcgcatcaaagctcgcaaggatcggac
accaaggagagattgtatgctcaaccgcaaaaagtgatgacgggagcgaaccaaggagat
gtgctcagagagcgcattaccgccgtcttgatggctgtgccagagggttctcgtgacga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TACACGTACGACCTCTGTGATCCAA 371 643 265 Antisense
TTCGACATTCGATCATTCTTCAATT 576 695 336 Antisense
GAGGGAACTCGTGTTCGAGCCATCG 81 401 364 Antisense
TATGCTGTTCAACATCACTCCAGTT 531 657 423 Antisense
ATTTCACGATCTTCGAGCTCGAGGC 685 13 461 Antisense
CGAGGCAGAAGCTGCACGTCTGTTC 559 249 480 Antisense
GAAGTCGGTCGACTCGGATGGCTTC 708 335 531 Antisense
GCTTCCAAGGAATGCTGGCACCACC 358 307 551 Antisense
AAGCTCGCAAGGATCGGACACCAAG 475 149 606 Antisense
CATTACCGCCGTCTTGATGGCTGTG 52 217 699 Antisense
CTGTGCCAGAGGGTTCTCGTGACGA 52 239 719 Antisense
Sequence Source Affymetrix Proprietary Database