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[176681_at]

Description F35B3.4 /REP_DB=WormBase Gene ID /WP=CE07171 /GB=AAK68375.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 19      6grp_high1_10_10 > 92      6grp_highest_nonorm > 55      6grp_total > 83      4grp_total > 49      6grp_highest_sub > 05      6grp_high1_sub > 04_05      6grp_total_sub > Hat      6grp_rule_based > S5      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3483
1 2 3 4 range
A 39.6
(-3)
A 		3490
(3)
P 		3264.1
(2.6)
P 		90.6
(-2.9)
A 		3450
B 33.8
(-3)
A 		2625.8
(1.7)
P 		3352.8
(3)
P 		40.6
(-3)
A 		3319
C 7.3
(-3)
A 		2739.1
(2.6)
P 		2929.5
(3)
P 		92.8
(-2.8)
A 		2922
avr 26.9
(-3) 		2951.6
(2.6) 		3182.1
(3) 		74.7
(-2.9) 		3155

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2408
1 2 3 4 5 6 range
A 314.2
(-3)
P 		1312.8
(-0)
P 		1367
(0.1)
P 		2000.3
(2)
P 		2343.1
(3)
P 		1787.3
(1.4)
P 		2029
B 347.8
(-3)
P 		1163.9
(-0.5)
P 		1493.2
(0.5)
P 		1677.8
(1.1)
P 		2296.3
(3)
P 		1259.5
(-0.2)
P 		1949
C 378.9
(-3)
P 		1079.8
(-1.2)
P 		1761.7
(0.5)
P 		1979.4
(1.1)
P 		2722.4
(3)
P 		1420.4
(-0.3)
P 		2344
avr 347
(-3) 		1185.5
(-0.6) 		1540.6
(0.4) 		1885.8
(1.4) 		2453.9
(3) 		1489.1
(0.3) 		2107
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1019
1 2 3 4 range
A 14.4
(-3)
A 		757.6
(2.3)
P 		853.3
(3)
P 		13.3
(-3)
A 		840
B 10.8
(-3)
A 		648.5
(2)
P 		776.9
(3)
P 		8.7
(-3)
A 		768
C 2.4
(-3)
A 		787.5
(1.6)
P 		1021
(3)
P 		15.6
(-2.9)
A 		1019
avr 9.2
(-3) 		731.2
(2) 		883.7
(3) 		12.5
(-3) 		875

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 875
1 2 3 4 5 6 range
A 57.9
(-3)
P 		466.9
(0.8)
P 		297.7
(-0.8)
P 		507.3
(1.2)
P 		707
(3)
P 		435.1
(0.5)
P 		649
B 121
(-3)
P 		387.4
(-1)
P 		362.6
(-1.2)
P 		444.4
(-0.6)
P 		933
(3)
P 		469.7
(-0.4)
P 		812
C 140.4
(-3)
P 		329.5
(-1.5)
P 		448.4
(-0.5)
P 		398.1
(-0.9)
P 		880.9
(3)
P 		475.5
(-0.3)
P 		741
avr 106.4
(-3) 		394.6
(-0.6) 		369.6
(-0.8) 		449.9
(-0.2) 		840.3
(3) 		460.1
(-0.1) 		734
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176681_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17928
Sequence Type Exemplar sequence
Representative Public ID F35B3.4 NCBI
Target Description F35B3.4 /REP_DB=WormBase Gene ID /WP=CE07171 /GB=AAK68375.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078349(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078349 NCBI Caenorhabditis elegans F35B3.4 (F35B3.4) mRNA, complete cds. 11/11 None
SNAP00000019166 ENSEMBL cdna:SNAP chromosome:CEL140:X:17019644:17020645:-1 11/11 None
GENEFINDER00000019173 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:17019644:17020645:-1 11/11 None
F35B3.4 ENSEMBL cdna:known chromosome:CEL140:X:17019552:17020677:-1 gene:F35B3.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:17019643-17020645(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F35B3.4
Entrez Gene 185255 Entrez gene
SwissProt Q966J9 EMBL-EBI
WormBase CE07171 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68375 Hypothetical protein F35B3.4 [Caenorhabditis elegans] ref|NP_510750.1| F35B3.4 [Caenorhabditis elegans] 1.0E-108
blast CAE69706 Hypothetical protein CBG15974 [Caenorhabditis briggsae] 1.0E-100
blast NP_501132 C42D4.3 [Caenorhabditis elegans] gb|AAA83347.1| Hypothetical protein C42D4.3 [Caenorhabditis elegans] 2.0E-67
Protein Domains
Database ID Description E-Value
Pfam IPR002602 EMBL-EBI Protein of unknown function DB 6.9E-58
Sequence
Target Sequence 
>C. ELEGANS:176681_AT
tccagtgtgctcgggaggttgcggaggaggttactcctgtggaagatatggctgtgcaaa
ggcgagaggatccaaaactctttcaatcaagagtgagaaaacaatgtcaccagacgagaa
gtttatgagatgctgccaggagagaaatcttccagatgcctgtctttccaagtgttcctt
ccgcacatacaccaagacagctctccaagccatgtacttcaagcaagactactgcccaat
gcaagcagcagcagatattcaatactgtgctgctcaagggcaagatcacactgactgctg
ctatagaaactcggttggaaccacattggccggaaacaaatgcttcactttctgcgacca
gcgtccaggaaacgtcacacaacttgacctctcctacttggcttgttacgatcgttttga
gaacatgaaggcctgcttttggcagaagctcaacgagcaatccaccccagagttctcatc
taacatcgattcagagtccgaattctctgagcaaggaccaaacagacacttcgcact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCAGTGTGCTCGGGAGGTTGCGGA 138 589 117 Antisense
AGAAATCTTCCAGATGCCTGTCTTT 690 71 259 Antisense
TTCCGCACATACACCAAGACAGCTC 195 699 295 Antisense
CAAGACAGCTCTCCAAGCCATGTAC 478 185 309 Antisense
GGGCAAGATCACACTGACTGCTGCT 676 493 394 Antisense
CTCGGTTGGAACCACATTGGCCGGA 66 233 426 Antisense
AAACAAATGCTTCACTTTCTGCGAC 85 129 450 Antisense
AAACGTCACACAACTTGACCTCTCC 232 127 486 Antisense
TCTCCTACTTGGCTTGTTACGATCG 33 609 506 Antisense
CACCCCAGAGTTCTCATCTAACATC 102 201 579 Antisense
AAGGACCAAACAGACACTTCGCACT 241 159 629 Antisense
Sequence Source Affymetrix Proprietary Database