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[176671_at]

Description F35B3.5 /REP_DB=WormBase Gene ID /WP=CE28298 /GB=AAK68376.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 11      6grp_high1_10_10 > 06      4grp_highest_8_8 > 51      4grp_high_8_8 > 32      6grp_highest_nonorm > 60      6grp_total > 07      4grp_total > 47      6grp_cgc > 20      6grp_high1_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 276
1 2 3 4 range
A 503.8
(3)
P 		265.2
(-2.7)
P 		252.7
(-3)
P 		362.7
(-0.4)
P 		251
B 489.1
(3)
P 		318.7
(-1.8)
P 		275.6
(-3)
P 		293.2
(-2.5)
P 		214
C 528.9
(3)
P 		277
(-2.8)
P 		324.2
(-1.7)
P 		267.5
(-3)
P 		261
avr 507.3
(3) 		287
(-2.9) 		284.2
(-3) 		307.8
(-2.4) 		223

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 542
1 2 3 4 5 6 range
A 667.1
(3)
P 		482.8
(0.7)
P 		319.5
(-1.3)
P 		385.3
(-0.5)
P 		184
(-3)
P 		427.4
(0)
P 		483
B 501.1
(3)
P 		448.7
(2.2)
P 		402.5
(1.4)
P 		396.9
(1.3)
P 		128.4
(-3)
P 		169.4
(-2.3)
P 		373
C 670
(3)
P 		366.8
(-1)
P 		400.5
(-0.6)
P 		309.6
(-1.7)
P 		236.1
(-2.7)
P 		214.6
(-3)
P 		455
avr 612.7
(3) 		432.8
(0.5) 		374.2
(-0.3) 		363.9
(-0.5) 		182.8
(-3) 		270.5
(-1.8) 		430
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 138
1 2 3 4 range
A 183.4
(3)
P 		57.6
(-2.8)
P 		66.1
(-2.4)
P 		53.1
(-3)
P 		130
B 156
(3)
P 		78.7
(-2)
P 		63.9
(-2.9)
P 		62.5
(-3)
P 		94
C 175.1
(3)
P 		79.6
(-1.4)
P 		113
(0.1)
P 		45.1
(-3)
P 		130
avr 171.5
(3) 		72
(-2.1) 		81
(-1.6) 		53.6
(-3) 		118

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 196
1 2 3 4 5 6 range
A 123
(0.5)
P 		171.7
(3)
P 		69.6
(-2.3)
P 		97.7
(-0.8)
P 		55.5
(-3)
P 		104.1
(-0.5)
P 		116
B 174.3
(3)
P 		149.4
(1.8)
P 		97.7
(-0.8)
P 		105.1
(-0.4)
P 		52.2
(-3)
P 		63.2
(-2.5)
P 		122
C 248.3
(3)
P 		111.9
(-1.4)
P 		101.9
(-1.7)
P 		62.3
(-3)
P 		76.4
(-2.5)
P 		71.8
(-2.7)
P 		186
avr 181.9
(3) 		144.3
(1.1) 		89.7
(-1.6) 		88.4
(-1.7) 		61.4
(-3) 		79.7
(-2.1) 		121
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176671_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17321
Sequence Type Exemplar sequence
Representative Public ID F35B3.5 NCBI
Target Description F35B3.5 /REP_DB=WormBase Gene ID /WP=CE28298 /GB=AAK68376.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078350(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078350 NCBI Caenorhabditis elegans Prion-like-(Q/N-rich)-domain-bearing protein family member (pqn-34) (pqn-34) mRNA, complete cds. 10/11 None
GENEFINDER00000019174 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:17022182:17032011:-1 10/11 None
F35B3.5a ENSEMBL cdna:known chromosome:CEL140:X:17021734:17030113:-1 gene:F35B3.5 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000019167 6/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
181743 2 1 NM_078350 176671_at (10)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:17022181-17029514(-) 98.19 100.0
Public Domain and Genome References
Ensembl F35B3.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68376 Prion-like-(q/n-rich)-domain-bearing protein protein 34, isoform a [Caenorhabditis elegans] ref|NP_510751.3| Prion-like-(Q/N-rich)-domain-bearing protein family member (pqn-34) [Caenorhabditis elegans] 0.0
blast CAE69707 Hypothetical protein CBG15975 [Caenorhabditis briggsae] 0.0
blast AAO21429 Prion-like-(q/n-rich)-domain-bearing protein protein 34, isoform b [Caenorhabditis elegans] ref|NP_872252.1| Prion-like-(Q/N-rich)-domain-bearing protein family member (pqn-34) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001888 EMBL-EBI Transposase, type 1 2.8E-41
Sequence
Target Sequence 
>C. ELEGANS:176671_AT
gaagaggacattctcaaccaccgttcatcaggacaaagtctcaaatgagtgaagtaacag
agtcttcaaggcagaatactcctagaatgagaggccagtcatctgttgagcaaagggtat
cggtgagcagtttggcggaaccaagtgagtataaaaaagtcaatcaagtgatgaaatttg
ttgcaccacacaagttatacgccaaaacagtgaccaaatccaaccgtggattaatcaata
acgctcttcaattcagtgtgttccctggagcagttaataatgcaacaaggcaggcgacaa
taacacaaatggcgtcatctagctcgaagcattttctgattcttttccgagaccaaaagt
gtcagtacagaggactgtacacttgggacgagatttccgacacagccgttaaaatttcgg
gacaaggaccgccaaaatgcactgaggcaatgatgaattcaatgtttaaatacgattccg
gagcgaaaaacttcacgaacattgccacaaagcacttgtctgccac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGAGGACATTCTCAACCACCGTT 395 337 2711 Antisense
AACCACCGTTCATCAGGACAAAGTC 695 145 2726 Antisense
GAGAGGCCAGTCATCTGTTGAGCAA 326 395 2799 Antisense
GTTGCACCACACAAGTTATACGCCA 533 439 2890 Antisense
AACGCTCTTCAATTCAGTGTGTTCC 584 145 2950 Antisense
AAATGGCGTCATCTAGCTCGAAGCA 694 119 3017 Antisense
GAAGCATTTTCTGATTCTTTTCCGA 51 343 3036 Antisense
ACAGAGGACTGTACACTTGGGACGA 144 97 3077 Antisense
TTGGGACGAGATTTCCGACACAGCC 460 709 3093 Antisense
GGGACAAGGACCGCCAAAATGCACT 533 489 3129 Antisense
TTGCCACAAAGCACTTGTCTGCCAC 305 701 3212 Antisense
Sequence Source Affymetrix Proprietary Database