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[176667_at]

Description F38E9.2 /REP_DB=WormBase Gene ID /WP=CE28306 /GB=AAA93348.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 06      4grp_total > 47      6grp_cgc > 12      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 143
1 2 3 4 range
A 157.2
(3)
P 		67.9
(-2.2)
A 		54.1
(-3)
A 		90.9
(-0.9)
A 		103
B 65.5
(3)
P 		57.7
(-1.1)
A 		54.3
(-2.9)
A 		54.1
(-3)
A 		11
C 79.5
(3)
A 		54.8
(0.7)
A 		48.4
(0.1)
A 		14.1
(-3)
A 		65
avr 100.7
(3) 		60.1
(-2) 		52.3
(-3) 		53
(-2.9) 		48

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 70
1 2 3 4 5 6 range
A 75.1
(3)
A 		56.5
(1.2)
A 		11.4
(-3)
A 		51.3
(0.8)
A 		39.5
(-0.4)
A 		67.2
(2.3)
P 		64
B 79
(3)
P 		55.7
(0.1)
A 		52.9
(-0.3)
A 		31.6
(-3)
A 		62.7
(0.9)
A 		43.7
(-1.5)
P 		47
C 61.1
(0.9)
P 		51.4
(-0.2)
A 		80.9
(3)
A 		48.3
(-0.5)
A 		48.7
(-0.5)
A 		25.3
(-3)
A 		56
avr 71.7
(3) 		54.5
(-0.7) 		48.4
(-2) 		43.7
(-3) 		50.3
(-1.6) 		45.4
(-2.6) 		28
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 55
1 2 3 4 range
A 57.2
(3)
P 		14.7
(-2.8)
A 		14.1
(-2.9)
A 		13.3
(-3)
A 		44
B 20.9
(3)
P 		14.3
(-1.2)
A 		12.6
(-2.3)
A 		11.5
(-3)
A 		9
C 26.3
(3)
A 		15.7
(0.3)
A 		16.9
(0.6)
A 		2.4
(-3)
A 		24
avr 34.8
(3) 		14.9
(-1.6) 		14.5
(-1.7) 		9.1
(-3) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 25
1 2 3 4 5 6 range
A 13.8
(0.9)
A 		20.1
(3)
A 		2.5
(-3)
A 		13
(0.6)
A 		11.9
(0.2)
A 		16.4
(1.7)
P 		18
B 27.5
(3)
P 		18.6
(0.2)
A 		12.8
(-1.6)
A 		8.4
(-3)
A 		25.5
(2.4)
A 		16.3
(-0.5)
P 		19
C 22.7
(3)
P 		15.7
(0)
A 		20.6
(2.1)
A 		9.7
(-2.5)
A 		15.8
(0.1)
A 		8.5
(-3)
A 		14
avr 21.3
(3) 		18.1
(1.2) 		12
(-2.1) 		10.4
(-3) 		17.7
(1) 		13.7
(-1.2) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176667_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17955
Sequence Type Exemplar sequence
Representative Public ID F38E9.2 NCBI
Target Description F38E9.2 /REP_DB=WormBase Gene ID /WP=CE28306 /GB=AAA93348.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078271(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078271 NCBI Caenorhabditis elegans Nematode AStacin protease family member (nas-39) (nas-39) mRNA, complete cds. 10/11 None
GENEFINDER00000030344 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:16462002:16471063:1 10/11 None
F38E9.2 ENSEMBL cdna:known chromosome:CEL140:X:16457346:16471853:1 gene:F38E9.2 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000030339 4/11 Cross Hyb Matching Probes None
SNAP00000030340 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:16461229-16471063(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nas-39
Ensembl F38E9.2
Entrez Gene 3565986 Entrez gene
SwissProt Q20176 EMBL-EBI
WormBase CE30977 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.14075.1.S1_S_AT similar to tolloid-like 1 cfa
DROSOPHILA_2:1641053_S_AT tolkin dm
DROSGENOME1:141301_AT tolkin dm
CHICKEN:GGA.239.1.S1_AT tolloid-like 1 gga
HG-U133_PLUS_2:206415_AT tolloid-like 1 hs
HG-U133A_2:206415_AT tolloid-like 1 hs
HG-FOCUS:206415_AT tolloid-like 1 hs
HG-U133A:206415_AT tolloid-like 1 hs
U133_X3P:HS2.129700.2.S1_3P_AT tolloid-like 1 hs
U133_X3P:HS.129700.0.S3_3P_AT tolloid-like 1 hs
HG-U133_PLUS_2:1555071_AT tolloid-like 1 hs
HG-U95AV2:31333_AT tolloid-like 1 hs
HG-U95E:86964_AT tolloid-like 1 hs
HG-U95B:42331_AT Tolloid-like 1 hs
HU35KSUBD:RC_R45345_AT Tolloid-like 1 hs
MG-U74AV2:98850_AT tolloid-like mm
MOUSE430_2:1420753_AT tolloid-like mm
MOUSE430A_2:1420753_AT tolloid-like mm
MOE430A:1420753_AT tolloid-like mm
MU11KSUBA:U34042_S_AT tolloid-like mm
MU19KSUBA:TC20753_AT Tolloid-like (Tll1), mRNA mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
8533 astacin activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA93348 Nematode astacin protease protein 39 [Caenorhabditis elegans] ref|NP_510672.2| Nematode AStacin protease family member (nas-39) [Caenorhabditis elegans] sp|Q20176|NAS39_CAEEL Zinc metalloproteinase nas-39 precursor (Nematode astacin 39) 0.0
blast CAE69967 Hypothetical protein CBG16363 [Caenorhabditis briggsae] 0.0
blast XP_393866 PREDICTED: similar to ENSANGP00000021251 [Apis mellifera] 0.0
blast CAC08820 colloid protein [Gallus gallus] ref|NP_990034.1| tolloid-like 1 [Gallus gallus] 1.0E-140
Protein Domains
Database ID Description E-Value
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 6.4E-106
Pfam IPR006209 EMBL-EBI EGF-like domain 4.1E-6
Pfam IPR006209 EMBL-EBI EGF-like domain 5.6E-7
Pfam IPR000859 EMBL-EBI CUB 1.7E-37
Pfam IPR000859 EMBL-EBI CUB 2.4E-41
Pfam IPR000859 EMBL-EBI CUB 2.5E-44
Pfam IPR000859 EMBL-EBI CUB 1.2E-38
Pfam IPR006209 EMBL-EBI EGF-like domain 4.1E-6
Pfam IPR006209 EMBL-EBI EGF-like domain 5.6E-7
Pfam IPR000859 EMBL-EBI CUB 1.7E-37
Pfam IPR000859 EMBL-EBI CUB 2.4E-41
Pfam IPR000859 EMBL-EBI CUB 2.5E-44
Pfam IPR000859 EMBL-EBI CUB 1.2E-38
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_510672.2 1 7-29
F38E9.2 1 7-29
Sequence
Target Sequence 
>C. ELEGANS:176667_AT
ccactccaggtcatcgtctgatgctgacattttctagttttcaagttgaagaacatgctc
aatgcaaatacgatgctgtgtccgtatatgacggcggagatggatcagcacagctagccg
gttggtctttttctgcgtgttctgcggcctggctccacccccactccttctatcgtcctc
caacgagctctatctcactttttcgtcggacgcgtcggtctctagacgaggctttcaggc
tcattacacttcttgactgctcatggattgtcagagcaaaatcgccgggacgtggcgtgc
ggattcagttctcaactttcaacattgaatctgaggagggatgtcagtatgactatattg
agatttacgatggacctgaagctacattggagcgccttgttggcaggttttgtggtgata
ctagtccggaagttataactagcacaggtcctgagctcctgcttatcatgcatacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCACTCCAGGTCATCGTCTGATGCT 358 275 1706 Antisense
AGATGGATCAGCACAGCTAGCCGGT 448 67 1803 Antisense
CAACGAGCTCTATCTCACTTTTTCG 562 185 1886 Antisense
ACGCGTCGGTCTCTAGACGAGGCTT 523 91 1915 Antisense
AGGCTTTCAGGCTCATTACACTTCT 537 53 1934 Antisense
TACACTTCTTGACTGCTCATGGATT 308 643 1950 Antisense
AAAATCGCCGGGACGTGGCGTGCGG 308 133 1983 Antisense
GTGCGGATTCAGTTCTCAACTTTCA 221 479 2002 Antisense
TGGAGCGCCTTGTTGGCAGGTTTTG 331 547 2093 Antisense
AACTAGCACAGGTCCTGAGCTCCTG 584 139 2142 Antisense
TGAGCTCCTGCTTATCATGCATACG 523 567 2157 Antisense
Sequence Source Affymetrix Proprietary Database