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[176660_s_at]

Description B0207.12A /REP_DB=WormBase Gene ID /WP=CE28278 /GEN=gbr-2 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 31      6grp_high1_10_10 > 03      4grp_highest_8_8 > 59      4grp_high_8_8 > 25      6grp_highest_nonorm > 40      6grp_total > 15      4grp_total > 47      6grp_highest_sub > De      6grp_cgc > 31      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 797
1 2 3 4 range
A 977.3
(3)
P 		364.3
(-2.4)
P 		296
(-3)
P 		384.8
(-2.2)
P 		681
B 1050.5
(3)
P 		429.6
(-2.2)
P 		335.2
(-3)
P 		490.7
(-1.7)
P 		715
C 1093.4
(3)
P 		438.2
(-2)
P 		308.6
(-3)
P 		479.4
(-1.7)
P 		785
avr 1040.4
(3) 		410.7
(-2.2) 		313.3
(-3) 		451.6
(-1.9) 		727

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 720
1 2 3 4 5 6 range
A 954.3
(3)
P 		693.9
(0.8)
P 		606.8
(0.1)
P 		446.3
(-1.2)
P 		404
(-1.6)
P 		234.1
(-3)
P 		720
B 822.9
(3)
P 		787.8
(2.5)
P 		624.1
(0.4)
P 		429.4
(-2.1)
P 		358.5
(-3)
P 		356.8
(-3)
P 		466
C 797.9
(3)
P 		733.4
(2.2)
P 		536.9
(-0)
P 		472.2
(-0.8)
P 		322.9
(-2.5)
P 		282.5
(-3)
P 		515
avr 858.4
(3) 		738.4
(1.7) 		589.3
(0.2) 		449.3
(-1.3) 		361.8
(-2.3) 		291.1
(-3) 		567
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 306
1 2 3 4 range
A 355.8
(3)
P 		79.1
(-2.5)
P 		77.4
(-2.6)
P 		56.3
(-3)
P 		300
B 335
(3)
P 		106.1
(-2.3)
P 		77.7
(-3)
P 		104.6
(-2.4)
P 		257
C 361.9
(3)
P 		126
(-2)
P 		107.5
(-2.4)
P 		80.8
(-3)
P 		281
avr 350.9
(3) 		103.7
(-2.5) 		87.5
(-2.8) 		80.6
(-3) 		270

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 239
1 2 3 4 5 6 range
A 175.9
(0.8)
P 		246.8
(3)
P 		132.1
(-0.6)
P 		113.2
(-1.2)
P 		121.9
(-0.9)
P 		57
(-3)
P 		190
B 286.3
(3)
P 		262.2
(2.2)
P 		151.5
(-1.7)
P 		113.7
(-3)
P 		145.7
(-1.9)
P 		133
(-2.3)
P 		173
C 295.7
(3)
P 		223.8
(0.9)
P 		136.6
(-1.7)
P 		95
(-3)
P 		104.5
(-2.7)
P 		94.6
(-3)
P 		201
avr 252.6
(3) 		244.3
(2.7) 		140.1
(-1.3) 		107.3
(-2.5) 		124
(-1.9) 		94.9
(-3) 		158
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176660_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1930
Sequence Type Exemplar sequence
Representative Public ID B0207.12A NCBI
Target Description B0207.12A /REP_DB=WormBase Gene ID /WP=CE28278 /GEN=gbr-2 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001025792(11), NM_001025791(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001025792 NCBI Caenorhabditis elegans altered AVeRmectin sensitivity family member (avr-14) (avr-14) mRNA, complete cds. 11/11 None
NM_001025791 NCBI Caenorhabditis elegans altered AVeRmectin sensitivity family member (avr-14) (avr-14) mRNA, complete cds. 11/11 None
GENEFINDER00000009182 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5975550:5981455:-1 11/11 None
B0207.12b ENSEMBL cdna:known chromosome:CEL140:I:5975380:5981455:-1 gene:B0207.12 11/11 None
B0207.12a ENSEMBL cdna:known chromosome:CEL140:I:5975380:5981455:-1 gene:B0207.12 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000009180 5/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
172270 3 2 NM_001025791 176660_s_at (11), 194145_x_at (9)
NM_001025792 176660_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5975549-5981455(-) 100.0 100.0
Public Domain and Genome References
Ensembl B0207.12
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68666 Altered avermectin sensitivity protein 14, isoform b [Caenorhabditis elegans] ref|NP_001020963.1| altered AVeRmectin sensitivity family member (avr-14) [Caenorhabditis elegans] 0.0
blast AAC25482 inhibitory amino acid receptor subunit gbr-2B [Caenorhabditis elegans] 0.0
blast CAA74623 GBR-2B protein [Haemonchus contortus] 0.0
blast AAK68665 Altered avermectin sensitivity protein 14, isoform a [Caenorhabditis elegans] ref|NP_001020962.1| altered AVeRmectin sensitivity family member (avr-14) [Caenorhabditis elegans] 0.0
blast AAC25481 inhibitory amino acid receptor subunit gbr-2A [Caenorhabditis elegans] 0.0
blast CAE60327 Hypothetical protein CBG03919 [Caenorhabditis briggsae] 0.0
blast C87791 protein B0207.12 [imported] - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006029 EMBL-EBI Neurotransmitter-gated ion-channel transmembrane region 4.5E-42
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 1.4E-62
Pfam IPR006029 EMBL-EBI Neurotransmitter-gated ion-channel transmembrane region 5.2E-39
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 6.3E-63
Pfam IPR006029 EMBL-EBI Neurotransmitter-gated ion-channel transmembrane region 4.5E-42
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001020963.1 4 255-277,284-303,318-340,403-425
NP_001020962.1 3 253-275,315-337,393-415
B0207.12b 4 255-277,284-303,318-340,403-425
B0207.12a 3 253-275,315-337,393-415
GENEFINDER00000009182 5 253-275,315-337,424-446,488-510,573-595
Sequence
Target Sequence 
>C. ELEGANS:176660_S_AT
aatacagttgtgcacgggtcgttttgcgtcttcgcagagaatacagttattatctcattc
aactttacattccatgtattatgcttgtcgttgtttcatgggtatctttctggcttgata
aggacgctgtgccagctcgtgtctctttgggagtaacaacacttctcacaatgactactc
aagccagtggaattaactcaaaattaccgcctgtttcttatattaaagctgttgatgtat
ggatcggagtgtgtcttgcattcatttttggagctcttctcgaatatgcagttgtcaatt
attacggaagaaaagaatttttgagaaaggaaaagaagaaaaaaacacgaattgatgatt
gtgtatgtccatcagatcgccctccacttcgattagatttatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATACAGTTGTGCACGGGTCGTTTT 704 167 1352 Antisense
GGTCGTTTTGCGTCTTCGCAGAGAA 581 501 1368 Antisense
TATCTCATTCAACTTTACATTCCAT 198 659 1402 Antisense
GTTTCATGGGTATCTTTCTGGCTTG 395 447 1444 Antisense
CCAGCTCGTGTCTCTTTGGGAGTAA 115 273 1483 Antisense
ACAACACTTCTCACAATGACTACTC 460 111 1507 Antisense
CTCAAAATTACCGCCTGTTTCTTAT 110 1 1548 Antisense
GGATCGGAGTGTGTCTTGCATTCAT 308 513 1592 Antisense
GGAGCTCTTCTCGAATATGCAGTTG 385 521 1621 Antisense
ATTGTGTATGTCCATCAGATCGCCC 220 5 1709 Antisense
GCCCTCCACTTCGATTAGATTTATC 185 287 1730 Antisense
Sequence Source Affymetrix Proprietary Database