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[176656_s_at]

Description Y71G12B.H_rc /REP_DB=WormBase Gene ID /WP=CE22897 /CHR=1 /FEA=Sanger Annotation /REVCOMP /DEF=(ST.LOUIS)
Cluster 4grp_high_8_8 > 36      6grp_total > 45      4grp_total > 44      6grp_total_sub > Cup      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 80
1 2 3 4 range
A 90
(-0.4)
P 		116.7
(3)
P 		70.2
(-3)
P 		72.1
(-2.8)
P 		47
B 95.2
(2.7)
P 		98
(3)
P 		61.8
(-0.5)
P 		36.8
(-3)
A 		61
C 66.8
(-3)
P 		86.4
(3)
P 		82.4
(1.8)
P 		66.9
(-3)
A 		20
avr 84
(0.6) 		100.4
(3) 		71.5
(-1.1) 		58.6
(-3) 		42

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 63
1 2 3 4 5 6 range
A 36.3
(0.7)
A 		44.3
(1.6)
P 		49.6
(2.2)
P 		56.8
(3)
A 		2.4
(-3)
A 		57
(3)
A 		55
B 65.7
(3)
P 		60.3
(2.5)
P 		60.7
(2.5)
A 		5.9
(-3)
A 		32.6
(-0.3)
A 		34.7
(-0.1)
P 		60
C 60.1
(3)
P 		46.9
(1.6)
P 		49.9
(1.9)
P 		4.8
(-3)
A 		5.4
(-2.9)
A 		33.9
(0.2)
A 		55
avr 54
(3) 		50.5
(2.5) 		53.4
(2.9) 		22.5
(-1.7) 		13.5
(-3) 		41.9
(1.2) 		41
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 25
1 2 3 4 range
A 32.8
(3)
P 		25.3
(1)
P 		18.4
(-0.9)
P 		10.6
(-3)
P 		22
B 30.4
(3)
P 		24.2
(1.4)
P 		14.3
(-1.3)
P 		7.8
(-3)
A 		23
C 22.1
(0.7)
P 		24.8
(1.7)
P 		28.7
(3)
P 		11.3
(-3)
A 		17
avr 28.4
(3) 		24.8
(1.8) 		20.5
(0.4) 		9.9
(-3) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 6.7
(-0.6)
A 		15.8
(3)
P 		10.8
(1)
P 		14.4
(2.4)
A 		0.7
(-3)
A 		13.9
(2.2)
A 		15
B 22.9
(3)
P 		20.1
(2.2)
P 		14.7
(0.7)
A 		1.6
(-3)
A 		13.2
(0.3)
A 		12.9
(0.2)
P 		21
C 22.3
(3)
P 		14.3
(0.7)
P 		12.7
(0.3)
P 		1
(-3)
A 		1.8
(-2.8)
A 		11.3
(-0.1)
A 		21
avr 17.3
(3) 		16.7
(2.7) 		12.7
(0.7) 		5.7
(-2.8) 		5.2
(-3) 		12.7
(0.7) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176656_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.28176
Sequence Type Exemplar sequence
Representative Public ID Y71G12B.H NCBI
Target Description Y71G12B.H_rc /REP_DB=WormBase Gene ID /WP=CE22897 /CHR=1 /FEA=Sanger Annotation /REVCOMP /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058485(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058485 NCBI Caenorhabditis elegans Y71G12B.11a (Y71G12B.11) mRNA, complete cds. 11/11 A 
GENEFINDER00000001829 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:1721598:1740273:1 11/11 None
SNAP00000001789 ENSEMBL cdna:SNAP chromosome:CEL140:I:1733299:1740273:1 11/11 None
Y71G12B.11a ENSEMBL cdna:known chromosome:CEL140:I:1721598:1740640:1 gene:Y71G12B.11 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
171736 2 1 NM_001026647 194260_x_at (11)
NM_058485 176329_at (11), 176650_s_at (11), 176656_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:1736515-1736878(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y71G12B.11
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK73909 Hypothetical protein Y71G12B.11a [Caenorhabditis elegans] ref|NP_490886.2| Y71G12B.11a [Caenorhabditis elegans] gb|AAA74747.1| talin 0.0
blast AAK73910 Hypothetical protein Y71G12B.11b [Caenorhabditis elegans] ref|NP_001021818.1| Y71G12B.11b [Caenorhabditis elegans] 0.0
blast CAE74421 Hypothetical protein CBG22153 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002558 EMBL-EBI I/LWEQ domain 6.7E-107
Pfam IPR000299 EMBL-EBI Band 4.1 9.1E-36
Pfam IPR002558 EMBL-EBI I/LWEQ domain 6.7E-107
Sequence
Target Sequence 
>C. ELEGANS:176656_S_AT
gcgcgatggattgctatgctagtggaaatgtgcagaaacatggtgcaaacgcagaacgtc
agcttctccaacaagttcaacatattgctagtcagctcgaggacaaggttgatgatcttc
ataatgccgcggtagaacatggtgaacgtctcccgaaggtggttcaactgcactgtcaaa
tggttgaggatctcgctgatgctgcgtgctgtgcagctggaatgactgttgatagcaacc
aacaaacggaacttttcgacaagtgtaagacggtagtcgaggcagaacttgcgatgatgg
tggctagccgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCGCGATGGATTGCTATGCTAGTGG 675 285 35 Antisense
GCAAACGCAGAACGTCAGCTTCTCC 143 319 79 Antisense
AGTTCAACATATTGCTAGTCAGCTC 571 63 108 Antisense
GTCAGCTCGAGGACAAGGTTGATGA 250 465 125 Antisense
GATGATCTTCATAATGCCGCGGTAG 157 411 145 Antisense
AACATGGTGAACGTCTCCCGAAGGT 177 137 170 Antisense
CGAAGGTGGTTCAACTGCACTGTCA 681 251 188 Antisense
GTTGAGGATCTCGCTGATGCTGCGT 177 439 217 Antisense
GATGCTGCGTGCTGTGCAGCTGGAA 709 413 232 Antisense
GACGGTAGTCGAGGCAGAACTTGCG 308 377 303 Antisense
ACTTGCGATGATGGTGGCTAGCCGT 76 101 321 Antisense
Sequence Source Affymetrix Proprietary Database