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[176650_s_at]

Description Y71G12B.G_rc /REP_DB=WormBase Gene ID /WP=CE22896 /CHR=1 /FEA=Sanger Annotation /REVCOMP /DEF=(ST.LOUIS)
Cluster 6grp_total > 16      4grp_total > 52      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 173
1 2 3 4 range
A 164
(2)
P 		193.4
(3)
P 		83.3
(-0.8)
A 		20.1
(-3)
A 		173
B 148.6
(3)
P 		105
(-0)
A 		80.5
(-1.7)
A 		62.3
(-3)
A 		86
C 137.9
(1.3)
P 		176
(3)
A 		127.4
(0.8)
A 		41.8
(-3)
A 		134
avr 150.2
(2.6) 		158.1
(3) 		97.1
(-0.1) 		41.4
(-3) 		117

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 133
1 2 3 4 5 6 range
A 32.4
(-1.1)
A 		84.9
(3)
A 		7.8
(-3)
A 		42.3
(-0.3)
A 		9.1
(-2.9)
A 		17.4
(-2.3)
A 		77
B 140.9
(3)
P 		54.1
(-1.5)
M 		24.7
(-3)
A 		26.6
(-2.9)
A 		27
(-2.9)
A 		36
(-2.4)
A 		116
C 122.4
(3)
P 		67.6
(-0.6)
A 		56.2
(-1.4)
A 		40.3
(-2.5)
A 		32.3
(-3)
A 		52.1
(-1.7)
A 		90
avr 98.6
(3) 		68.9
(0.6) 		29.6
(-2.5) 		36.4
(-1.9) 		22.8
(-3) 		35.2
(-2) 		76
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 57
1 2 3 4 range
A 59.7
(3)
P 		42
(1.1)
P 		21.8
(-1)
A 		2.9
(-3)
A 		57
B 47.4
(3)
P 		25.9
(-0.8)
A 		18.7
(-2)
A 		13.3
(-3)
A 		34
C 45.6
(2.3)
P 		50.6
(3)
A 		44.4
(2.1)
A 		7
(-3)
A 		44
avr 50.9
(3) 		39.5
(1.4) 		28.3
(-0.1) 		7.7
(-3) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 47
1 2 3 4 5 6 range
A 6
(-2.1)
A 		30.2
(3)
A 		1.7
(-3)
A 		10.7
(-1.1)
A 		2.7
(-2.8)
A 		4.2
(-2.5)
A 		29
B 49
(3)
P 		18
(-1.3)
M 		6
(-3)
A 		7
(-2.9)
A 		11
(-2.3)
A 		13.4
(-2)
A 		43
C 45.4
(3)
P 		20.6
(-1)
A 		14.3
(-2)
A 		8.1
(-3)
A 		10.5
(-2.6)
A 		17.4
(-1.5)
A 		37
avr 33.5
(3) 		22.9
(0.6) 		7.3
(-3) 		8.6
(-2.7) 		8.1
(-2.8) 		11.7
(-2) 		26
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176650_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.28173
Sequence Type Exemplar sequence
Representative Public ID Y71G12B.G NCBI
Target Description Y71G12B.G_rc /REP_DB=WormBase Gene ID /WP=CE22896 /CHR=1 /FEA=Sanger Annotation /REVCOMP /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058485(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058485 NCBI Caenorhabditis elegans Y71G12B.11a (Y71G12B.11) mRNA, complete cds. 11/11 A 
GENEFINDER00000001829 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:1721598:1740273:1 11/11 None
SNAP00000001789 ENSEMBL cdna:SNAP chromosome:CEL140:I:1733299:1740273:1 11/11 None
Y71G12B.11a ENSEMBL cdna:known chromosome:CEL140:I:1721598:1740640:1 gene:Y71G12B.11 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
171736 2 1 NM_001026647 194260_x_at (11)
NM_058485 176329_at (11), 176650_s_at (11), 176656_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:1737706-1738033(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y71G12B.11
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK73909 Hypothetical protein Y71G12B.11a [Caenorhabditis elegans] ref|NP_490886.2| Y71G12B.11a [Caenorhabditis elegans] gb|AAA74747.1| talin 0.0
blast AAK73910 Hypothetical protein Y71G12B.11b [Caenorhabditis elegans] ref|NP_001021818.1| Y71G12B.11b [Caenorhabditis elegans] 0.0
blast CAE74421 Hypothetical protein CBG22153 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002558 EMBL-EBI I/LWEQ domain 6.7E-107
Pfam IPR000299 EMBL-EBI Band 4.1 9.1E-36
Pfam IPR002558 EMBL-EBI I/LWEQ domain 6.7E-107
Sequence
Target Sequence 
>C. ELEGANS:176650_S_AT
gagactcaagtccttgtgatccacgcggttcgtgacgttgccgcagcgctcacctctctg
attcaagccaccaagaacgcctcgggactttcccttcaacatccagcaatgggacatctc
aaagatgcggcaaaggttatggtttccaacgttgctcgtctcctcaagacggtggccact
gtcgaagagaagaatcaacagggaacacgagcagtcgaggcagcagtagatgctattggc
tttgagatgaagcaattcgagcatgatctcaatgag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGACTCAAGTCCTTGTGATCCACG 654 393 28 Antisense
TGATCCACGCGGTTCGTGACGTTGC 530 565 44 Antisense
CCTCTCTGATTCAAGCCACCAAGAA 34 269 80 Antisense
TTTCCCTTCAACATCCAGCAATGGG 119 671 116 Antisense
GATGCGGCAAAGGTTATGGTTTCCA 497 411 151 Antisense
TTATGGTTTCCAACGTTGCTCGTCT 518 679 164 Antisense
TTGCTCGTCTCCTCAAGACGGTGGC 213 703 179 Antisense
AGACGGTGGCCACTGTCGAAGAGAA 197 71 194 Antisense
GGAACACGAGCAGTCGAGGCAGCAG 55 529 229 Antisense
GTAGATGCTATTGGCTTTGAGATGA 172 457 253 Antisense
GCAATTCGAGCATGATCTCAATGAG 205 323 279 Antisense
Sequence Source Affymetrix Proprietary Database