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[176648_at]

Description C32E8.10C /REP_DB=WormBase Gene ID /WP=CE24822 /GEN=unc-11 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 26      4grp_total > 20      6grp_cgc > 04      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 77
1 2 3 4 range
A 56.4
(1.4)
A 		74.2
(3)
A 		8.5
(-3)
A 		63.6
(2)
A 		66
B 42.7
(3)
A 		27.2
(-3)
A 		32.2
(-1.1)
A 		33.5
(-0.6)
P 		16
C 10.7
(-2.7)
A 		10.4
(-2.7)
A 		7.1
(-3)
A 		83.6
(3)
P 		77
avr 36.6
(-0.2) 		37.3
(-0.1) 		15.9
(-3) 		60.2
(3) 		44

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 96
1 2 3 4 5 6 range
A 99.3
(3)
A 		61.4
(0.5)
P 		27.8
(-1.6)
A 		6.6
(-3)
A 		42
(-0.7)
A 		29.4
(-1.5)
A 		93
B 4.4
(-3)
A 		40.5
(3)
A 		31.8
(1.6)
A 		17.7
(-0.8)
A 		21.6
(-0.1)
A 		8
(-2.4)
A 		36
C 16.7
(-0)
A 		27
(2.3)
A 		3.3
(-3)
A 		4.3
(-2.8)
A 		9.3
(-1.7)
A 		30.2
(3)
A 		27
avr 40.1
(2.5) 		43
(3) 		21
(-0.9) 		9.5
(-3) 		24.3
(-0.3) 		22.5
(-0.7) 		34
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 20.6
(3)
A 		16.1
(1.5)
A 		2.2
(-3)
A 		9.3
(-0.7)
A 		18
B 13.6
(3)
A 		6.7
(-3)
A 		7.5
(-2.3)
A 		7.1
(-2.7)
P 		7
C 3.6
(-2.4)
A 		3
(-2.7)
A 		2.5
(-3)
A 		14.1
(3)
P 		12
avr 12.6
(3) 		8.6
(0.2) 		4.1
(-3) 		10.2
(1.3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 21
1 2 3 4 5 6 range
A 18.3
(2)
A 		21.8
(3)
P 		6.1
(-1.7)
A 		1.7
(-3)
A 		12.7
(0.3)
A 		7.2
(-1.4)
A 		20
B 1.5
(-3)
A 		13.5
(3)
A 		7.7
(0.1)
A 		4.7
(-1.4)
A 		8.8
(0.7)
A 		3
(-2.3)
A 		12
C 6.2
(0.5)
A 		8.2
(1.8)
A 		0.8
(-3)
A 		0.9
(-2.9)
A 		3
(-1.6)
A 		10.1
(3)
A 		9
avr 8.7
(0.1) 		14.5
(3) 		4.9
(-1.8) 		2.4
(-3) 		8.2
(-0.1) 		6.8
(-0.8) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176648_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.254
Sequence Type Exemplar sequence
Representative Public ID C32E8.10C NCBI
Target Description C32E8.10C /REP_DB=WormBase Gene ID /WP=CE24822 /GEN=unc-11 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058827(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058827 NCBI Caenorhabditis elegans UNCoordinated family member (unc-11) (unc-11) mRNA, complete cds. 11/11 None
GENEFINDER00000040668 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3799207:3806810:-1 11/11 None
C32E8.10c ENSEMBL cdna:known chromosome:CEL140:I:3798463:3805720:-1 gene:C32E8.10 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000040657 8/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
171952 7 1 NM_001025843 172028_x_at (10), 172029_x_at (11), 172150_x_at (10), 172151_x_at (10), 176649_s_at (11)
NM_001025845 172028_x_at (11), 172029_x_at (11), 172150_x_at (10), 172151_x_at (10), 176649_s_at (11)
NM_058826 172028_x_at (10), 172029_x_at (11), 172149_x_at (11), 172150_x_at (10), 172151_x_at (10), 172152_x_at (11), 176649_s_at (11)
NM_058827 172028_x_at (10), 172029_x_at (11), 172149_x_at (11), 172150_x_at (10), 172151_x_at (10), 172152_x_at (11), 176648_at (11), 176649_s_at (11)
NM_058828 172028_x_at (10), 172029_x_at (9), 172150_x_at (11), 172151_x_at (9), 176649_s_at (11)
NM_058829 172028_x_at (10), 172029_x_at (11), 172149_x_at (11), 172150_x_at (10), 172151_x_at (10), 172152_x_at (11), 176649_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3799206-3805706(-) 98.53 100.0
Public Domain and Genome References
Ensembl C32E8.10
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68214 Uncoordinated protein 11, isoform c [Caenorhabditis elegans] ref|NP_491228.1| UNCoordinated family member (unc-11) [Caenorhabditis elegans] 0.0
blast T25603 hypothetical protein C32E8.10 - Caenorhabditis elegans 0.0
blast AAK68212 Uncoordinated protein 11, isoform a [Caenorhabditis elegans] ref|NP_491227.1| UNCoordinated family member (unc-11) [Caenorhabditis elegans] gb|AAD37365.1| AP180-like adaptor protein [Caenorhabditis elegans] sp|Q9XZI6|PICA_CAEEL Phosphatidylinositol-binding clathrin assembly protein unc-11 (AP180-like adaptor protein) (Uncoordinated protein 11) 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001026 EMBL-EBI Epsin N-terminal homology 1.1E-44
Pfam IPR001026 EMBL-EBI Epsin N-terminal homology 1.1E-44
Sequence
Target Sequence 
>C. ELEGANS:176648_AT
cgcaagctccaaatgagcccgaaaacccatttataactgctcccgcggcaccacaaatgc
acaatgcgccgcccgtcccaccaccaccagcatctcagggggctccagctcctatcaatc
cttttgctgatccttcagctactgctgcttcttcagcacaaccgtttggggatccagatg
acttcaaattt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGCAAGCTCCAAATGAGCCCGAAAA 620 253 1376 Antisense
GCTCCAAATGAGCCCGAAAACCCAT 92 297 1381 Antisense
GAAAACCCATTTATAACTGCTCCCG 363 335 1396 Antisense
CCAGCTCCTATCAATCCTTTTGCTG 490 273 1480 Antisense
GCTCCTATCAATCCTTTTGCTGATC 13 299 1483 Antisense
TATCAATCCTTTTGCTGATCCTTCA 662 659 1488 Antisense
TTGCTGATCCTTCAGCTACTGCTGC 680 701 1499 Antisense
CTTCTTCAGCACAACCGTTTGGGGA 77 221 1523 Antisense
CAGCACAACCGTTTGGGGATCCAGA 306 203 1529 Antisense
ACCGTTTGGGGATCCAGATGACTTC 592 21 1536 Antisense
TGGGGATCCAGATGACTTCAAATTT 274 553 1542 Antisense
Sequence Source Affymetrix Proprietary Database