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[176646_at]

Description R144.9 /REP_DB=WormBase Gene ID /WP=CE25092 /GB=AAK21456.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 44      6grp_high1_10_10 > 44      4grp_highest_8_8 > 04      4grp_high_8_8 > 37      6grp_highest_nonorm > 71      6grp_total > 40      4grp_total > 35      6grp_highest_sub > 02      6grp_cgc > 72      6grp_high1_sub > 02      6grp_cgc_sub > 02      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 142
1 2 3 4 range
A 363
(-0.6)
P 		400
(3)
P 		338.4
(-3)
P 		354
(-1.5)
P 		62
B 347.8
(-3)
P 		349.9
(-2.8)
P 		352.9
(-2.6)
P 		429
(3)
P 		81
C 335.3
(1.5)
P 		352.1
(3)
P 		350.4
(2.8)
P 		287
(-3)
P 		65
avr 348.7
(-2.6) 		367.3
(3) 		347.2
(-3) 		356.7
(-0.2) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 129
1 2 3 4 5 6 range
A 306.1
(-2.8)
P 		374
(0.9)
P 		413.4
(3)
P 		368.3
(0.6)
P 		302.9
(-3)
P 		343.4
(-0.8)
P 		111
B 299.3
(-2.6)
P 		394.7
(3)
P 		321.8
(-1.3)
P 		385.2
(2.4)
P 		300.3
(-2.6)
P 		292.9
(-3)
P 		102
C 362.6
(1.5)
P 		347.1
(0.6)
P 		331.6
(-0.3)
P 		388.8
(3)
P 		284
(-3)
P 		365.1
(1.6)
P 		105
avr 322.7
(-1.1) 		371.9
(2.4) 		355.6
(1.2) 		380.8
(3) 		295.7
(-3) 		333.8
(-0.3) 		85
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 84
1 2 3 4 range
A 132.2
(3)
P 		86.8
(-0.4)
P 		88.5
(-0.3)
P 		51.8
(-3)
P 		80
B 110.9
(3)
P 		86.4
(-2.1)
P 		81.8
(-3)
P 		91.4
(-1)
P 		29
C 111
(2.1)
P 		101.2
(1.3)
P 		122.1
(3)
P 		48.4
(-3)
P 		74
avr 118
(3) 		91.5
(0.1) 		97.5
(0.7) 		63.9
(-3) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 78
1 2 3 4 5 6 range
A 56.4
(-3)
P 		133
(3)
P 		90
(-0.4)
P 		93.4
(-0.1)
P 		91.4
(-0.3)
P 		83.6
(-0.9)
P 		77
B 104.1
(-0.1)
P 		131.4
(3)
P 		78.1
(-3)
P 		102
(-0.3)
P 		122
(1.9)
P 		109.2
(0.5)
P 		53
C 134.4
(3)
P 		105.9
(-0)
P 		84.4
(-2.3)
P 		78.2
(-3)
P 		91.9
(-1.5)
P 		122.2
(1.7)
P 		56
avr 98.3
(-0.8) 		123.4
(3) 		84.2
(-3) 		91.2
(-1.9) 		101.8
(-0.3) 		105
(0.2) 		39
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176646_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8037
Sequence Type Exemplar sequence
Representative Public ID R144.9 NCBI
Target Description R144.9 /REP_DB=WormBase Gene ID /WP=CE25092 /GB=AAK21456.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065665(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065665 NCBI Caenorhabditis elegans MeDiaTor family member (mdt-11) (mdt-11) mRNA, complete cds. 11/11 None
SNAP00000019426 ENSEMBL cdna:SNAP chromosome:CEL140:III:5021187:5021850:1 11/11 None
GENEFINDER00000019437 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5021187:5021850:1 11/11 None
R144.9 ENSEMBL cdna:known chromosome:CEL140:III:5021187:5021857:1 gene:R144.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5021185-5021849(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol mdt-11
Ensembl R144.9
Entrez Gene 175682 Entrez gene
SwissProt Q9BI74 EMBL-EBI
WormBase CE25092 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_498066 MeDiaTor family member (mdt-11) [Caenorhabditis elegans] gb|AAK21456.1| Mediator protein 11 [Caenorhabditis elegans] 2.0E-71
blast T16757 hypothetical protein R144.3 - Caenorhabditis elegans 5.0E-60
blast CAE70083 Hypothetical protein CBG16524 [Caenorhabditis briggsae] 4.0E-49
Sequence
Target Sequence 
>C. ELEGANS:176646_AT
cccatccgatcctgttctaacagatcggattcaagcaatcgtgacgactgaaaaaagtat
tgatgagatgatgaagtgtgctcgagaaattattcaagatttgggaaaagagaaacaaat
cggaaaaaataaaatggaagataatgcgaacaatttcaaaaaactgataactcaagtgga
aaatgaattgagcgctcaaatgcaatacttatcgcatgtttgtgttggatcatcacatca
aggatcaacattcggtgtacttcaaaactcacttctcgctcaatctggactttcttctct
tcattcggaactttttcaaatcgtgagatatcttgatccaacatcagatgaaccacaaac
tacagaagaagatgaggaggacggatccgatgatctgaatgaagatggagcagatggtgc
tccatcttcgacagtgacgtcatcgactactgatggtagcggtggaggtgatgacgctgc
aagtagcagtgctccgcgaagtcaagaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCCATCCGATCCTGTTCTAACAGAT 450 259 24 Antisense
GAGCGCTCAAATGCAATACTTATCG 50 387 213 Antisense
ATACTTATCGCATGTTTGTGTTGGA 393 23 228 Antisense
AAGGATCAACATTCGGTGTACTTCA 183 159 263 Antisense
GTGTACTTCAAAACTCACTTCTCGC 126 487 278 Antisense
CTTCTCTTCATTCGGAACTTTTTCA 330 221 317 Antisense
GCTCCATCTTCGACAGTGACGTCAT 53 299 442 Antisense
GTGACGTCATCGACTACTGATGGTA 413 483 457 Antisense
CGGTGGAGGTGATGACGCTGCAAGT 124 245 483 Antisense
GACGCTGCAAGTAGCAGTGCTCCGC 473 369 496 Antisense
TAGCAGTGCTCCGCGAAGTCAAGAA 632 651 507 Antisense
Sequence Source Affymetrix Proprietary Database