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[176638_s_at]

Description R144.6 /REP_DB=WormBase Gene ID /WP=CE26980 /GB=AAK21460.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 96      4grp_total > 24      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 212
1 2 3 4 range
A 86.4
(-3)
A 		163.3
(-0.2)
A 		178.5
(0.4)
P 		249.4
(3)
P 		163
B 105.3
(-3)
A 		154.8
(-1.1)
P 		182.1
(-0)
P 		259.8
(3)
A 		155
C 122.7
(-3)
A 		163.4
(-1.6)
P 		199.6
(-0.4)
P 		298
(3)
P 		175
avr 104.8
(-3) 		160.5
(-1) 		186.7
(-0) 		269.1
(3) 		164

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 593
1 2 3 4 5 6 range
A 72.8
(-3)
A 		96.6
(-2.7)
P 		151.8
(-2)
P 		315.1
(0.1)
P 		452.3
(1.8)
P 		543.2
(3)
P 		470
B 68
(-3)
A 		72.6
(-3)
A 		199.1
(-1.6)
P 		279.7
(-0.7)
P 		445.2
(1)
P 		631.4
(3)
P 		563
C 45.5
(-3)
A 		106.6
(-2.4)
A 		187.4
(-1.6)
P 		354.5
(0.1)
P 		417.4
(0.8)
P 		638.3
(3)
P 		593
avr 62.1
(-3) 		91.9
(-2.7) 		179.4
(-1.7) 		316.4
(-0.2) 		438.3
(1.2) 		604.3
(3) 		542
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 38
1 2 3 4 range
A 31.4
(-3)
A 		35.4
(-1.4)
A 		46.7
(3)
P 		36.5
(-1)
P 		15
B 33.6
(-3)
A 		38.2
(-1.7)
P 		42.2
(-0.6)
P 		55.4
(3)
A 		22
C 40.6
(-3)
A 		47
(-1.7)
P 		69.6
(3)
P 		50.2
(-1)
P 		29
avr 35.2
(-3) 		40.2
(-1.3) 		52.8
(3) 		47.4
(1.2) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 222
1 2 3 4 5 6 range
A 13.4
(-3)
A 		34.4
(-2)
P 		33.1
(-2)
P 		79.9
(0.2)
P 		136.5
(3)
P 		132.2
(2.8)
P 		123
B 23.7
(-3)
A 		24.1
(-3)
A 		48.3
(-2.3)
P 		74.1
(-1.6)
P 		180.9
(1.5)
P 		235.4
(3)
P 		212
C 16.9
(-3)
A 		32.5
(-2.5)
A 		47.7
(-2.1)
P 		71.3
(-1.3)
P 		135.1
(0.6)
P 		213.7
(3)
P 		197
avr 18
(-3) 		30.3
(-2.6) 		43
(-2.1) 		75.1
(-1.1) 		150.8
(1.5) 		193.8
(3) 		176
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176638_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7535
Sequence Type Exemplar sequence
Representative Public ID R144.6 NCBI
Target Description R144.6 /REP_DB=WormBase Gene ID /WP=CE26980 /GB=AAK21460.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065660(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065660 NCBI Caenorhabditis elegans R144.10 (R144.10) mRNA, complete cds. 11/11 None
GENEFINDER00000019432 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5001433:5004682:-1 11/11 None
R144.10 ENSEMBL cdna:known chromosome:CEL140:III:5001331:5002272:-1 gene:R144.10 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000019420 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5001431-5006925(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R144.10
Entrez Gene 187902 Entrez gene
SwissProt Q9BI75 EMBL-EBI
WormBase CE26946 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast T16753 hypothetical protein R144.6 - Caenorhabditis elegans 0.0
blast NP_498062 R144.6 [Caenorhabditis elegans] gb|AAU87817.1| Hypothetical protein R144.6 [Caenorhabditis elegans] sp|Q10000|YRR6_CAEEL Hypothetical protein R144.6 in chromosome III 1.0E-176
blast CAE70087 Hypothetical protein CBG16528 [Caenorhabditis briggsae] 1.0E-157
blast NP_498061 R144.10 [Caenorhabditis elegans] gb|AAK21459.1| Hypothetical protein R144.10 [Caenorhabditis elegans] 1.0E-114
blast T16753 hypothetical protein R144.6 - Caenorhabditis elegans 1.0E-104
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000019432 8 2-24,34-56,69-91,116-138,193-215,230-252,265-283,293-315
Sequence
Target Sequence 
>C. ELEGANS:176638_S_AT
attcatcgccaatatcgtccacgatgaagaatggagacgagattacagtaaagataccaa
aaaatacctttcttggatgccaactacccacgtcatcggcaaagaggacttcgaaaatct
gcttaaacacccgactgttgctgacaaagtggattttatttcaggaagtgttgatcttgt
tctttcgaacatgtacgtgaaatgcaataattcagaatatatggtgtgcaaagtgagaat
cccggacattgataaaccagaaatcatctgtcactggccaactgaaccaaaggctcatta
taaaaagtcaaaacgatcaatgacagagccagacgaagaagatgaccgagttttagaaca
acatgaaaaagaaatcgctcatctcgtggcggaaaacactaatttgcgtcaagagctcgc
cattctgaagcagtcttttgaagagttcaagaaggcggctgaattggctagaaatcgtga
caaggcagacttttgggctga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTCATCGCCAATATCGTCCACGAT 522 11 1995 Antisense
TACCTTTCTTGGATGCCAACTACCC 236 617 2059 Antisense
CTACCCACGTCATCGGCAAAGAGGA 36 225 2078 Antisense
GAAAATCTGCTTAAACACCCGACTG 483 353 2107 Antisense
CACCCGACTGTTGCTGACAAAGTGG 195 201 2122 Antisense
GGTGTGCAAAGTGAGAATCCCGGAC 148 499 2217 Antisense
ATCTGTCACTGGCCAACTGAACCAA 671 31 2260 Antisense
GAAATCGCTCATCTCGTGGCGGAAA 482 361 2365 Antisense
AATTTGCGTCAAGAGCTCGCCATTC 417 173 2395 Antisense
GAGCTCGCCATTCTGAAGCAGTCTT 352 387 2407 Antisense
GTGACAAGGCAGACTTTTGGGCTGA 248 483 2471 Antisense
Sequence Source Affymetrix Proprietary Database