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[176632_s_at]

Description C27D11.1 /REP_DB=WormBase Gene ID /WP=CE00543 /GEN=egl-45 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 63      6grp_high1_10_10 > 35      4grp_highest_8_8 > 10      4grp_high_8_8 > 54      6grp_highest_nonorm > 67      6grp_total > 36      4grp_total > 57      6grp_highest_sub > Cu      6grp_cgc > 25      6grp_high1_sub > 02      6grp_total_sub > Cup      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1321
1 2 3 4 range
A 2661.2
(-0.2)
P 		2917.6
(1)
P 		3337.4
(3)
P 		2070.1
(-3)
P 		1267
B 2422.7
(-3)
P 		3177.5
(1.7)
P 		3390.8
(3)
P 		2679
(-1.4)
P 		968
C 2528
(-1.5)
P 		3007.9
(2)
P 		3150
(3)
P 		2322
(-3)
P 		828
avr 2537.3
(-1.8) 		3034.3
(1.3) 		3292.7
(3) 		2357
(-3) 		936

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1727
1 2 3 4 5 6 range
A 1956.7
(0.2)
P 		2646.8
(3)
P 		1348.9
(-2.3)
P 		1727.8
(-0.7)
P 		1176
(-3)
P 		2402.9
(2)
P 		1471
B 1899.2
(3)
P 		1898.7
(3)
P 		1506.6
(0.6)
P 		1840.8
(2.6)
P 		919.7
(-3)
P 		1585.9
(1.1)
P 		980
C 2509.5
(3)
P 		1947
(-0.2)
P 		2382.9
(2.3)
P 		1537.7
(-2.5)
P 		1658.3
(-1.8)
P 		1456.1
(-3)
P 		1053
avr 2121.8
(2.7) 		2164.2
(3) 		1746.1
(0.3) 		1702.1
(-0) 		1251.3
(-3) 		1815
(0.7) 		913
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 795
1 2 3 4 range
A 968.9
(3)
P 		633.3
(-0)
P 		872.5
(2.1)
P 		303
(-3)
P 		666
B 772.6
(2.6)
P 		784.8
(3)
P 		785.7
(3)
P 		570.9
(-3)
P 		215
C 836.8
(0.8)
P 		864.8
(1)
P 		1097.9
(3)
P 		391.5
(-3)
P 		706
avr 859.4
(2.3) 		761
(1.1) 		918.7
(3) 		421.8
(-3) 		497

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 648
1 2 3 4 5 6 range
A 360.7
(-2.4)
P 		941.3
(3)
P 		293.8
(-3)
P 		438.2
(-1.7)
P 		354.9
(-2.4)
P 		585
(-0.3)
P 		648
B 660.8
(3)
P 		632
(2.4)
P 		365.8
(-3)
P 		487.6
(-0.5)
P 		373.6
(-2.8)
P 		591.4
(1.6)
P 		295
C 930.2
(3)
P 		594.1
(-0.2)
P 		606.5
(-0.1)
P 		309.2
(-3)
P 		536.6
(-0.8)
P 		487.4
(-1.3)
P 		621
avr 650.6
(1.6) 		722.5
(3) 		422
(-2.8) 		411.7
(-3) 		421.7
(-2.8) 		554.6
(-0.2) 		311
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176632_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7058
Sequence Type Exemplar sequence
Representative Public ID C27D11.1 NCBI
Target Description C27D11.1 /REP_DB=WormBase Gene ID /WP=CE00543 /GEN=egl-45 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066297(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066297 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-45) (egl-45) mRNA, complete cds. 11/11 None
C27D11.1 ENSEMBL cdna:known chromosome:CEL140:III:7824306:7828548:-1 gene:C27D11.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7824615-7828508(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol egl-45
Ensembl C27D11.1
Entrez Gene 176094 Entrez gene
SwissProt P34339 EMBL-EBI
WormBase CE00543 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:254873_AT eukaryotic translation initiation factor 3 subunit 10 / eIF-3 theta / eIF3a (TIF3A1) at
CANINE:1606087_AT similar to eukaryotic translation initiation factor 3, subunit 10 (theta) cfa
CANINE:1588841_AT similar to eukaryotic translation initiation factor 3, subunit 10 (theta) cfa
CANINE_2:CFA.16750.1.S1_AT similar to eukaryotic translation initiation factor 3, subunit 10 (theta) cfa
CANINE_2:CFAAFFX.18749.1.S1_S_AT similar to eukaryotic translation initiation factor 3, subunit 10 (theta) cfa
DROSOPHILA_2:1631404_AT dm
DROSGENOME1:149279_AT dm
CHICKEN:GGAAFFX.22876.1.S1_AT similar to eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa; eukaryotic translation initiation factor 3, subunit 10 (theta, 170kD); Eukaryotic translation initiation factor 3, subunit 10, 170kD; eukaryotic translation initia... gga
CHICKEN:GGAAFFX.22889.1.S1_S_AT similar to eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa; eukaryotic translation initiation factor 3, subunit 10 (theta, 170kD); Eukaryotic translation initiation factor 3, subunit 10, 170kD; eukaryotic translation initia... gga
HG-U133_PLUS_2:200595_S_AT eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa hs
HG-U133A:200595_S_AT eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa hs
HG-FOCUS:200595_S_AT eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa hs
HG-U133A_2:200595_S_AT eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa hs
HG-U133_PLUS_2:200596_S_AT eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa hs
HG-U133A_2:200596_S_AT eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa hs
HG-U133A:200596_S_AT eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa hs
HG-U133_PLUS_2:200597_AT eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa hs
HG-U133A_2:200597_AT eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa hs
HG-U133A:200597_AT eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa hs
HUGENEFL:U58046_S_AT eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa hs
U133_X3P:G4503508_3P_A_AT eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa hs
U133_X3P:HS.198899.0.S3_3P_AT eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa hs
U133_X3P:HS.198899.0.S1_3P_X_AT eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa hs
U133_X3P:HS.198899.0.S1_3P_A_AT eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa hs
HG-U95AV2:32785_AT eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa hs
HG-U95E:72966_AT eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa hs
U133_X3P:HS.283861.0.S1_3P_AT Eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa hs
HG-U133_PLUS_2:234466_AT Eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa hs
HG-U133B:234466_AT Eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa hs
HG-U133_PLUS_2:234342_AT Eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa hs
HG-U133B:234342_AT Eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa hs
MOE430A:1416660_AT eukaryotic translation initiation factor 3, subunit 10 (theta) mm
MOUSE430A_2:1416660_AT eukaryotic translation initiation factor 3, subunit 10 (theta) mm
MOUSE430_2:1416660_AT eukaryotic translation initiation factor 3, subunit 10 (theta) mm
MOE430A:1416661_AT eukaryotic translation initiation factor 3, subunit 10 (theta) mm
MOUSE430A_2:1416661_AT eukaryotic translation initiation factor 3, subunit 10 (theta) mm
MOUSE430_2:1416661_AT eukaryotic translation initiation factor 3, subunit 10 (theta) mm
MOUSE430_2:1448425_AT eukaryotic translation initiation factor 3, subunit 10 (theta) mm
MOE430A:1448425_AT eukaryotic translation initiation factor 3, subunit 10 (theta) mm
MOUSE430A_2:1448425_AT eukaryotic translation initiation factor 3, subunit 10 (theta) mm
MOUSE430A_2:1416659_AT eukaryotic translation initiation factor 3, subunit 10 (theta) mm
MOE430A:1416659_AT eukaryotic translation initiation factor 3, subunit 10 (theta) mm
MOUSE430_2:1416659_AT eukaryotic translation initiation factor 3, subunit 10 (theta) mm
MU11KSUBA:U14172_S_AT eukaryotic translation initiation factor 3, subunit 10 (theta) mm
MG-U74AV2:94250_AT eukaryotic translation initiation factor 3, subunit 10 (theta) mm
RG-U34B:RC_AA956907_G_AT eukaryotic translation initiation factor 3, subunit 10 (theta) (predicted) rn
RG-U34B:RC_AA956907_AT eukaryotic translation initiation factor 3, subunit 10 (theta) (predicted) rn
RAE230A:1389968_AT eukaryotic translation initiation factor 3, subunit 10 (theta) (predicted) rn
RAT230_2:1389968_AT eukaryotic translation initiation factor 3, subunit 10 (theta) (predicted) rn
RAE230B:1379485_AT eukaryotic translation initiation factor 3, subunit 10 (theta) (predicted) rn
RAT230_2:1379485_AT eukaryotic translation initiation factor 3, subunit 10 (theta) (predicted) rn
RG-U34B:RC_AA997389_AT Eukaryotic translation initiation factor 3, subunit 10 (theta) (predicted) rn
RG-U34B:RC_AA957623_AT Eukaryotic translation initiation factor 3, subunit 10 (theta) (predicted) rn
YEAST_2:1771444_AT Subunit of the core complex of translation initiation factor 3(eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes Sc
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
45182 translation regulator activity inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA27955 Egg laying defective protein 45 [Caenorhabditis elegans] ref|NP_498698.1| EGg Laying defective family member (egl-45) [Caenorhabditis elegans] sp|P34339|IF3A_CAEEL Probable eukaryotic translation initiation factor 3 subunit 10 (eIF-3 theta) (eIF3a) 0.0
blast CAE57182 Hypothetical protein CBG00021 [Caenorhabditis briggsae] 0.0
blast AAH54342 EIF3S10 protein [Homo sapiens] 1.0E-148
Sequence
Target Sequence 
>C. ELEGANS:176632_S_AT
aacccaccacgtgagagtttgcctccaagagaaacgagaccaatgcgtgatggaccaaca
cgtgaacctcgagaattccgtggagatcgtgatcgtgaacctagagagccattccgtgag
gtgccatcttctaaagctgatactgacaactcttggagaagttctgctcaaccaactcgt
aaacctgacgatcgccgcagcgacgagttccggcgcaacgatgatgtccgtcgcaacgac
gatgttcgccgcaatgatccaccccgtccagcatcgaaggctgatactggggataagtgg
gaacgaggagtgaagccagtcgtttctccaccaaagaccgatgccccatcagtttctgaa
ccgaaatcggaaggacccaagaggttcgtgccaccccatcttcgaaacagacaaggaggt
ggtggagcaggtggttccgaggaacaatccgctcctgctagatctggaaattccaatgtg
acaagcccaccagaccgggctcaaggactccgaggcccaccaccaactggccgcaactcg
ttgcctcgtcgtgacggaccaccaccacggtctaacaacactgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACCCACCACGTGAGAGTTTGCCTC 544 143 2620 Antisense
TGATGGACCAACACGTGAACCTCGA 61 567 2667 Antisense
GTGAACCTAGAGAGCCATTCCGTGA 645 481 2714 Antisense
ATTCCGTGAGGTGCCATCTTCTAAA 370 5 2730 Antisense
CCGGCGCAACGATGATGTCCGTCGC 647 263 2829 Antisense
CAACGACGATGTTCGCCGCAATGAT 461 187 2853 Antisense
GGAGTGAAGCCAGTCGTTTCTCCAC 656 515 2926 Antisense
GAAGGACCCAAGAGGTTCGTGCCAC 186 159 2989 Antisense
ATCCGCTCCTGCTAGATCTGGAAAT 364 39 3066 Antisense
TCCAATGTGACAAGCCCACCAGACC 283 585 3091 Antisense
CACCACCACGGTCTAACAACACTGG 575 201 3179 Antisense
Sequence Source Affymetrix Proprietary Database