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[176631_s_at]

Description Y47D7A.8 /REP_DB=WormBase Gene ID /WP=CE22057 /GEN=skr-14 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 77      4grp_total > 09      6grp_cgc > 48      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 68
1 2 3 4 range
A 2
(-3)
A 		6.8
(-1.8)
A 		25.2
(3)
A 		12.1
(-0.4)
A 		23
B 2.9
(-2.6)
A 		12.5
(-0.4)
A 		1.1
(-3)
A 		27.9
(3)
A 		27
C 18.2
(-2.6)
A 		18.9
(-2.5)
A 		14.1
(-3)
A 		68.9
(3)
A 		55
avr 7.7
(-3) 		12.7
(-2) 		13.5
(-1.8) 		36.3
(3) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 239
1 2 3 4 5 6 range
A 1.2
(-3)
A 		3.8
(-2.9)
A 		4.6
(-2.9)
A 		1.5
(-3)
A 		48.1
(-1.2)
A 		158.7
(3)
P 		158
B 17.1
(-2.7)
A 		6.5
(-3)
A 		8.5
(-2.9)
A 		26.5
(-2.5)
A 		59.6
(-1.6)
A 		240
(3)
P 		234
C 3.7
(-3)
A 		2.5
(-3)
A 		2.4
(-3)
A 		2.2
(-3)
A 		25
(-2.4)
A 		224.9
(3)
P 		223
avr 7.3
(-2.9) 		4.3
(-3) 		5.2
(-3) 		10.1
(-2.8) 		44.2
(-1.8) 		207.9
(3) 		204
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 0.7
(-3)
A 		1.5
(-2.2)
A 		6.6
(3)
A 		1.8
(-1.9)
A 		6
B 0.9
(-2.3)
A 		3.1
(0.1)
A 		0.2
(-3)
A 		5.9
(3)
A 		6
C 6
(-2)
A 		5.4
(-2.6)
A 		4.9
(-3)
A 		11.6
(3)
A 		7
avr 2.5
(-3) 		3.3
(-1.8) 		3.9
(-0.8) 		6.4
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 89
1 2 3 4 5 6 range
A 0.2
(-3)
A 		1.4
(-2.8)
A 		1
(-2.9)
A 		0.4
(-3)
A 		14.5
(-0.8)
A 		38.6
(3)
P 		38
B 5.9
(-2.7)
A 		2.2
(-3)
A 		2.1
(-3)
A 		7
(-2.7)
A 		24.2
(-1.5)
A 		89.5
(3)
P 		87
C 1.4
(-2.9)
A 		0.8
(-3)
A 		0.6
(-3)
A 		0.4
(-3)
A 		8.1
(-2.4)
A 		75.3
(3)
P 		75
avr 2.5
(-2.9) 		1.5
(-3) 		1.2
(-3) 		2.6
(-2.9) 		15.6
(-1.7) 		67.8
(3) 		67
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176631_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15421
Sequence Type Exemplar sequence
Representative Public ID Y47D7A.8 NCBI
Target Description Y47D7A.8 /REP_DB=WormBase Gene ID /WP=CE22057 /GEN=skr-14 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071819(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071819 NCBI Caenorhabditis elegans SKp1 Related (ubiquitin ligase complex component) family member (skr-14) (skr-14) mRNA, complete cds. 11/11 None
SNAP00000043262 ENSEMBL cdna:SNAP chromosome:CEL140:V:4420883:4421891:-1 11/11 None
Y47D7A.8 ENSEMBL cdna:known chromosome:CEL140:V:4420811:4421891:-1 gene:Y47D7A.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
AF440515 8/11 Cross Hyb Matching Probes None
GENEFINDER00000043281 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:4420882-4421891(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol skr-14
Ensembl Y47D7A.8
Entrez Gene 178839 Entrez gene
SwissProt Q9N3U9 EMBL-EBI
WormBase CE22057 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF60635 Skp1 related (ubiquitin ligase complex component) protein 14 [Caenorhabditis elegans] ref|NP_504220.3| SKp1 Related (ubiquitin ligase complex component) family member (skr-14) [Caenorhabditis elegans] 1.0E-108
blast AAL34103 SKR-14 [Caenorhabditis elegans] 4.0E-94
blast NP_503045 SKp1 Related (ubiquitin ligase complex component) family member (skr-12) [Caenorhabditis elegans] gb|AAK77208.1| Skp1 related (ubiquitin ligase complex component) protein 12 [Caenorhabditis elegans] gb|AAL34101.1| SKR-12 [Caenorhabditis elegans] gb|AAB17536.1| homolog to Skp1p, an evolutionarily conserved kinetochore protein in budding yeast [Caenorhabditis elegans] 1.0E-82
Protein Domains
Database ID Description E-Value
Pfam IPR001232 EMBL-EBI SKP1 component 4.5E-40
Pfam IPR001232 EMBL-EBI SKP1 component 1.4E-29
Sequence
Target Sequence 
>C. ELEGANS:176631_S_AT
gaacagactgttttcgctaaacgacgagaacgctgcaaatgtaacccatcggacgcgcga
atcgacatactcatcaccatgtctgctccagtttccgatgctccagccgcccaaatcttt
tacaagatcatttcaaacgatggagtcgtgacaaaaatgagcgagaaagccgtccaacaa
tcgaagactctcagcaatctcattgagaatctcggttataccattgaaaacattgagacc
cgtgatccaattccagttactaatgtgaatggaaagactatggagaaggtcgctgaatgg
tgtgagaagcacaacgccgatgcaattccagaggacaatatgaatgttctcaagaccctc
accattccagaatgggatcaaaagttcttgaaaattgaggatgaagctctgttcgatttg
attctggcgtcaaacttccttgacatcaagggtcttatgtactatggttgtaagactgta
tccaacatggcaaagggcaaaacgacagctgaactcag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACAGACTGTTTTCGCTAAACGAC 481 351 22 Antisense
GCTGCAAATGTAACCCATCGGACGC 400 287 53 Antisense
TCGGACGCGCGAATCGACATACTCA 666 603 70 Antisense
GACATACTCATCACCATGTCTGCTC 271 371 85 Antisense
CAGCCGCCCAAATCTTTTACAAGAT 310 201 125 Antisense
TGAGCGAGAAAGCCGTCCAACAATC 620 565 179 Antisense
CGAAGACTCTCAGCAATCTCATTGA 539 253 203 Antisense
GGAGAAGGTCGCTGAATGGTGTGAG 501 517 303 Antisense
CTCTGTTCGATTTGATTCTGGCGTC 470 231 428 Antisense
ATCAAGGGTCTTATGTACTATGGTT 598 23 466 Antisense
AGGGCAAAACGACAGCTGAACTCAG 131 57 515 Antisense
Sequence Source Affymetrix Proprietary Database