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[176627_s_at]

Description C36E6.F /REP_DB=WormBase Gene ID /WP=CE20545 /CHR=X /FEA=Sanger Annotation /DEF=locus:mlc-1 (ST.LOUIS)
Cluster 6grp_highest_10_10 > 03      6grp_high1_10_10 > 21      4grp_highest_8_8 > 49      4grp_high_8_8 > 41      6grp_highest_nonorm > 17      6grp_total > 32      4grp_total > 54      6grp_highest_sub > De      6grp_cgc > 70      6grp_high1_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3435
1 2 3 4 range
A 12757.1
(3)
P 		12673.1
(2.7)
P 		11109.5
(-3)
P 		11152.8
(-2.8)
P 		1648
B 11854.8
(3)
P 		10116.2
(-2.3)
P 		10887
(0)
P 		9904.6
(-3)
P 		1950
C 12334.2
(3)
P 		9999.3
(-1.7)
P 		10105.2
(-1.4)
P 		9322.2
(-3)
P 		3012
avr 12315.4
(3) 		10929.5
(-0.8) 		10700.6
(-1.4) 		10126.5
(-3) 		2189

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8171
1 2 3 4 5 6 range
A 11755
(1.1)
P 		12082.6
(1.5)
P 		12470.6
(1.9)
P 		13636.9
(3)
P 		8853.9
(-1.7)
P 		7537.4
(-3)
P 		6100
B 11359.7
(2)
P 		10865.8
(1.5)
P 		12367.9
(3)
P 		9522
(0.2)
P 		9661.3
(0.4)
P 		6202.1
(-3)
P 		6166
C 14373.1
(3)
P 		10425.2
(0)
P 		13684.5
(2.5)
P 		9640.5
(-0.6)
P 		14002
(2.7)
P 		6405.5
(-3)
P 		7968
avr 12495.9
(2.7) 		11124.5
(1.3) 		12841
(3) 		10933.1
(1.1) 		10839.1
(1) 		6715
(-3) 		6126
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3073
1 2 3 4 range
A 4644.9
(3)
P 		2751
(-0.8)
P 		2904.2
(-0.5)
P 		1632.6
(-3)
P 		3012
B 3780.4
(3)
P 		2498.5
(-1.6)
P 		2522.8
(-1.5)
P 		2110.7
(-3)
P 		1670
C 4083
(3)
P 		2874.8
(0.1)
P 		3522
(1.7)
P 		1571.9
(-3)
P 		2511
avr 4169.4
(3) 		2708.1
(-0.7) 		2983
(0) 		1771.7
(-3) 		2398

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3492
1 2 3 4 5 6 range
A 2166.9
(-2.2)
P 		4297.2
(3)
P 		2715.8
(-0.9)
P 		3458.6
(1)
P 		2671.7
(-1)
P 		1835.1
(-3)
P 		2462
B 3952.6
(3)
P 		3616.8
(1.8)
P 		3003.3
(-0.5)
P 		2522
(-2.2)
P 		3925.3
(2.9)
P 		2312.7
(-3)
P 		1640
C 5327.5
(3)
P 		3181
(-0.8)
P 		3482.9
(-0.3)
P 		1938.7
(-3)
P 		4530.8
(1.6)
P 		2144.3
(-2.6)
P 		3389
avr 3815.7
(3) 		3698.3
(2.6) 		3067.3
(0.4) 		2639.8
(-1.1) 		3709.3
(2.6) 		2097.4
(-3) 		1718
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176627_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19564
Sequence Type Exemplar sequence
Representative Public ID C36E6.F NCBI
Target Description C36E6.F /REP_DB=WormBase Gene ID /WP=CE20545 /CHR=X /FEA=Sanger Annotation /DEF=locus:mlc-1 (ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078427(11), NM_078428(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078427 NCBI Caenorhabditis elegans Myosin Light Chain family member (mlc-2) (mlc-2) mRNA, complete cds. 11/11 A 
NM_078428 NCBI Caenorhabditis elegans Myosin Light Chain family member (mlc-1) (mlc-1) mRNA, complete cds. 11/11 A 
SNAP00000036942 ENSEMBL cdna:SNAP chromosome:CEL140:X:17455812:17456478:-1 11/11 None
GENEFINDER00000036960 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:17455812:17456478:-1 11/11 None
SNAP00000036943 ENSEMBL cdna:SNAP chromosome:CEL140:X:17459385:17460067:1 11/11 None
GENEFINDER00000036964 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:17459385:17460067:1 11/11 None
C36E6.5.3 ENSEMBL cdna:known chromosome:CEL140:X:17455618:17456505:-1 gene:C36E6.5 11/11 None
C36E6.5.2 ENSEMBL cdna:known chromosome:CEL140:X:17455638:17456505:-1 gene:C36E6.5 11/11 A 
C36E6.5.1 ENSEMBL cdna:known chromosome:CEL140:X:17455638:17456497:-1 gene:C36E6.5 11/11 A 
C36E6.5.4 ENSEMBL cdna:known chromosome:CEL140:X:17455716:17456502:-1 gene:C36E6.5 11/11 None
C36E6.3 ENSEMBL cdna:known chromosome:CEL140:X:17459085:17460757:1 gene:C36E6.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:17456859-17460066(+) 100.0 100.0
Public Domain and Genome References
Gene Title

Gene Symbol mlc-1
mlc-2
Ensembl C36E6.5
C36E6.3
Entrez Gene 181775 Entrez gene
181776 Entrez gene
SwissProt P19626 EMBL-EBI
WormBase CE34269 Wormbase
CE20542 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.21284.1.S1_AT similar to myosin light chain 2, precursor lymphocyte-specific cfa
CANINE:1590988_S_AT similar to Myosin regulatory light chain 2, skeletal muscle isoform (G2) (DTNB) (MLC-2) cfa
CANINE:1591371_S_AT similar to Myosin regulatory light chain 2, skeletal muscle isoform (G2) (DTNB) (MLC-2) cfa
CANINE_2:CFA.1238.8.S1_S_AT similar to Myosin regulatory light chain 2, skeletal muscle isoform (G2) (DTNB) (MLC-2) cfa
CHICKEN:GGA.13194.1.S1_AT similar to myosin L2B regulatory light chain, cardiac muscle - chicken gga
HG-U133_PLUS_2:221659_S_AT myosin light chain 2, precursor lymphocyte-specific hs
HG-U133A:221659_S_AT myosin light chain 2, precursor lymphocyte-specific hs
HG-U133A_2:221659_S_AT myosin light chain 2, precursor lymphocyte-specific hs
U133_X3P:G12803868_3P_A_AT myosin light chain 2, precursor lymphocyte-specific hs
HG-U133_PLUS_2:221660_AT myosin light chain 2, precursor lymphocyte-specific hs
HG-U133A:221660_AT myosin light chain 2, precursor lymphocyte-specific hs
HG-U133A_2:221660_AT myosin light chain 2, precursor lymphocyte-specific hs
HG-U133_PLUS_2:205163_AT fast skeletal myosin light chain 2 hs
HG-U133A:205163_AT fast skeletal myosin light chain 2 hs
HG-FOCUS:205163_AT fast skeletal myosin light chain 2 hs
HG-U133A_2:205163_AT fast skeletal myosin light chain 2 hs
HUGENEFL:M21812_AT fast skeletal myosin light chain 2 hs
U133_X3P:G7019426_3P_AT fast skeletal myosin light chain 2 hs
HG-U95AV2:34881_AT fast skeletal myosin light chain 2 hs
MOE430A:1420805_AT myosin light chain 2, precursor lymphocyte-specific mm
MOUSE430_2:1420805_AT myosin light chain 2, precursor lymphocyte-specific mm
MOUSE430A_2:1420805_AT myosin light chain 2, precursor lymphocyte-specific mm
MU11KSUBB:X65979_S_AT myosin light chain 2, precursor lymphocyte-specific mm
MG-U74AV2:101195_AT myosin light chain 2, precursor lymphocyte-specific mm
MU11KSUBA:U77943_S_AT myosin light chain, phosphorylatable, fast skeletal muscle mm
MU11KSUBB:MSA.17147.0_F_AT myosin light chain, phosphorylatable, fast skeletal muscle mm
MU11KSUBB:MSA.3233.0_F_AT myosin light chain, phosphorylatable, fast skeletal muscle mm
MU11KSUBB:MSA.24240.0_S_AT myosin light chain, phosphorylatable, fast skeletal muscle mm
MG-U74AV2:92881_AT myosin light chain, phosphorylatable, fast skeletal muscle mm
MG-U74AV2:162101_F_AT myosin light chain, phosphorylatable, fast skeletal muscle mm
MG-U74CV2:170347_F_AT myosin light chain, phosphorylatable, fast skeletal muscle mm
MOE430A:1448371_AT myosin light chain, phosphorylatable, fast skeletal muscle mm
MOUSE430A_2:1448371_AT myosin light chain, phosphorylatable, fast skeletal muscle mm
MOUSE430_2:1448371_AT myosin light chain, phosphorylatable, fast skeletal muscle mm
MU11KSUBB:MSA.30406.0_F_AT Myosin light chain, phosphorylatable, fast skeletal muscle (Mylpf), mRNA mm
MU11KSUBB:MSA.6060.0_AT Myosin light chain, phosphorylatable, fast skeletal muscle (Mylpf), mRNA mm
MU19KSUBA:TC22501_AT Myosin light chain, phosphorylatable, fast skeletal muscle (Mylpf), mRNA mm
MU19KSUBA:TC22502_F_AT Myosin light chain, phosphorylatable, fast skeletal muscle (Mylpf), mRNA mm
RG-U34A:X00975_AT myosin, light polypeptide 2 rn
RG-U34A:X00975_G_AT myosin, light polypeptide 2 rn
RAE230A:1387787_AT myosin, light polypeptide 2 rn
RAT230_2:1387787_AT myosin, light polypeptide 2 rn
RG-U34B:RC_AA998118_AT Myosin, light polypeptide 2 rn
RG-U34C:RC_AI230218_AT Myosin, light polypeptide 2 rn
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5859 muscle myosin inferred from direct assay QuickGO AmiGO
5859 muscle myosin inferred from sequence similarity QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK09069 Myosin light chain protein 2 [Caenorhabditis elegans] ref|NP_510828.1| Myosin Light Chain family member (mlc-2) [Caenorhabditis elegans] sp|P19626|MLR2_CAEEL Myosin regulatory light chain 2 gb|AAA28114.1| myosin light chain 2.0E-81
blast AAK09070 Myosin light chain protein 1 [Caenorhabditis elegans] ref|NP_510829.2| Myosin Light Chain family member (mlc-1) [Caenorhabditis elegans] sp|P19625|MLR1_CAEEL Myosin regulatory light chain 1 gb|AAA28113.1| myosin light chain 2.0E-81
Protein Domains
Database ID Description E-Value
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.7E-6
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.3
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.2
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.7E-6
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.2
Sequence
Target Sequence 
>C. ELEGANS:176627_S_AT
agaagtcctcaaagaagcgctcgggatccgaggccgcccaattcgaccagaagaccatcc
aggaattcaaggaggctttcggaatcatggatcagaacaaggacggaataattgacaagt
ctgacctcaaggatctctacgcttccatgggacaaatcgccccagacagccagatcgacg
ccatgattaaagaggcttccggaccaatcaacttcaccgtcttcctcacccttttcggag
agagattgacaggaactgacccagaggccaccattattggagcctttgccatgttcgaca
agaaggactgcggaaagatcaaggaggacgaccttatcaagatccttcaaaaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAAGTCCTCAAAGAAGCGCTCGGG 58 75 332 Antisense
AAGAAGCGCTCGGGATCCGAGGCCG 265 153 343 Antisense
TTCGGAATCATGGATCAGAACAAGG 701 681 409 Antisense
GACAAGTCTGACCTCAAGGATCTCT 266 365 445 Antisense
AGTCTGACCTCAAGGATCTCTACGC 279 61 449 Antisense
CATGGGACAAATCGCCCCAGACAGC 648 207 477 Antisense
GAGAGATTGACAGGAACTGACCCAG 479 393 571 Antisense
TGACAGGAACTGACCCAGAGGCCAC 558 571 578 Antisense
GATCAAGGAGGACGACCTTATCAAG 26 423 648 Antisense
GGACGACCTTATCAAGATCCTTCAA 74 525 657 Antisense
GACCTTATCAAGATCCTTCAAAACA 357 381 661 Antisense
Sequence Source Affymetrix Proprietary Database