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[176626_at]

Description C36E6.F /REP_DB=WormBase Gene ID /WP=CE20545 /CHR=X /FEA=Sanger Annotation /DEF=locus:mlc-1 (ST.LOUIS)
Cluster 6grp_highest_10_10 > 03      6grp_high1_10_10 > 22      4grp_highest_8_8 > 16      4grp_high_8_8 > 52      6grp_highest_nonorm > 22      6grp_total > 32      4grp_total > 59      6grp_highest_sub > De      6grp_cgc > 80      6grp_high1_sub > De      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2347
1 2 3 4 range
A 1344.1
(1)
P 		1875.4
(3)
P 		1819.2
(2.8)
P 		268.6
(-3)
P 		1607
B 1527.1
(2.1)
P 		1747.5
(3)
P 		1535.5
(2.1)
P 		271.3
(-3)
P 		1476
C 1995.5
(1.5)
P 		2576.8
(3)
P 		2421.6
(2.6)
P 		229.5
(-3)
P 		2347
avr 1622.2
(1.5) 		2066.6
(3) 		1925.4
(2.5) 		256.5
(-3) 		1810

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1910
1 2 3 4 5 6 range
A 1351.9
(-1.5)
P 		1803.2
(1.3)
P 		2079.5
(3)
P 		1282.1
(-1.9)
P 		1550.7
(-0.2)
P 		1100.8
(-3)
P 		979
B 1228
(-0.4)
P 		1862.6
(1.9)
P 		2167.3
(3)
P 		1059.8
(-1)
P 		991.8
(-1.2)
P 		491
(-3)
P 		1676
C 2400.7
(3)
P 		1017.3
(-1.8)
P 		1068.6
(-1.7)
P 		708.6
(-2.9)
P 		1804.1
(0.9)
P 		684.4
(-3)
P 		1716
avr 1660.2
(2.3) 		1561
(1.8) 		1771.8
(3) 		1016.8
(-1.5) 		1448.9
(1.1) 		758.7
(-3) 		1013
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 805
1 2 3 4 range
A 489.4
(3)
P 		407.1
(1.9)
P 		475.6
(2.8)
P 		39.3
(-3)
P 		450
B 487
(3)
P 		431.6
(2.2)
P 		355.8
(1.2)
P 		57.8
(-3)
P 		429
C 660.6
(1.6)
P 		740.8
(2.2)
P 		844
(3)
P 		38.7
(-3)
P 		805
avr 545.7
(2.9) 		526.5
(2.6) 		558.5
(3) 		45.3
(-3) 		513

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 747
1 2 3 4 5 6 range
A 249.2
(-3)
P 		641.3
(3)
P 		452.9
(0.1)
P 		325.2
(-1.8)
P 		467.9
(0.3)
P 		268
(-2.7)
P 		392
B 427.3
(0.4)
P 		620
(3)
P 		526.3
(1.7)
P 		280.7
(-1.7)
P 		403
(0)
P 		183.1
(-3)
P 		437
C 889.8
(3)
P 		310.4
(-1.7)
P 		272
(-2)
P 		142.5
(-3)
P 		583.8
(0.5)
P 		229.1
(-2.3)
P 		747
avr 522.1
(3) 		523.9
(3) 		417.1
(0.8) 		249.5
(-2.5) 		484.9
(2.2) 		226.7
(-3) 		297
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176626_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19564
Sequence Type Exemplar sequence
Representative Public ID C36E6.F NCBI
Target Description C36E6.F /REP_DB=WormBase Gene ID /WP=CE20545 /CHR=X /FEA=Sanger Annotation /DEF=locus:mlc-1 (ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078428
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000036960 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:17455812:17456478:-1 None
SNAP00000036943 ENSEMBL cdna:SNAP chromosome:CEL140:X:17459385:17460067:1 None
NM_078428 NCBI Caenorhabditis elegans Myosin Light Chain family member (mlc-1) (mlc-1) mRNA, complete cds. A 
C36E6.3 ENSEMBL cdna:known chromosome:CEL140:X:17459085:17460757:1 gene:C36E6.3 A B 
GENEFINDER00000036964 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:17459385:17460067:1 None
SNAP00000036942 ENSEMBL cdna:SNAP chromosome:CEL140:X:17455812:17456478:-1 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:17456859-17460066(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol mlc-1
Ensembl C36E6.3
Entrez Gene 181776 Entrez gene
WormBase CE34269 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.21284.1.S1_AT similar to myosin light chain 2, precursor lymphocyte-specific cfa
CHICKEN:GGA.13194.1.S1_AT similar to myosin L2B regulatory light chain, cardiac muscle - chicken gga
HG-U133_PLUS_2:221659_S_AT myosin light chain 2, precursor lymphocyte-specific hs
HG-U133A:221659_S_AT myosin light chain 2, precursor lymphocyte-specific hs
HG-U133A_2:221659_S_AT myosin light chain 2, precursor lymphocyte-specific hs
U133_X3P:G12803868_3P_A_AT myosin light chain 2, precursor lymphocyte-specific hs
HG-U133_PLUS_2:221660_AT myosin light chain 2, precursor lymphocyte-specific hs
HG-U133A:221660_AT myosin light chain 2, precursor lymphocyte-specific hs
HG-U133A_2:221660_AT myosin light chain 2, precursor lymphocyte-specific hs
MOE430A:1420805_AT myosin light chain 2, precursor lymphocyte-specific mm
MOUSE430_2:1420805_AT myosin light chain 2, precursor lymphocyte-specific mm
MOUSE430A_2:1420805_AT myosin light chain 2, precursor lymphocyte-specific mm
MU11KSUBB:X65979_S_AT myosin light chain 2, precursor lymphocyte-specific mm
MG-U74AV2:101195_AT myosin light chain 2, precursor lymphocyte-specific mm
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5859 muscle myosin inferred from direct assay QuickGO AmiGO
5859 muscle myosin inferred from sequence similarity QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK09070 Myosin light chain protein 1 [Caenorhabditis elegans] ref|NP_510829.2| Myosin Light Chain family member (mlc-1) [Caenorhabditis elegans] sp|P19625|MLR1_CAEEL Myosin regulatory light chain 1 gb|AAA28113.1| myosin light chain 2.0E-81
blast AAK09069 Myosin light chain protein 2 [Caenorhabditis elegans] ref|NP_510828.1| Myosin Light Chain family member (mlc-2) [Caenorhabditis elegans] sp|P19626|MLR2_CAEEL Myosin regulatory light chain 2 gb|AAA28114.1| myosin light chain 2.0E-81
Protein Domains
Database ID Description E-Value
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.7E-6
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.2
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.3
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.7E-6
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.2
Sequence
Target Sequence 
>C. ELEGANS:176626_AT
aactgtttggcttcgaatattttctcaagtacatctctctcaaaagcagttagtctcagt
tcactactcagtacagtccacaaaatgtccaaggccgccaagaagaagtcctcaaagaag
cgctcgggatccgaggccgcccaattcgaccagaagaccatccaggaattcaaggaggct
ttcggaatcatggatcagaacaaggacggaataattgacaagtctgacctcaaggatctc
tacgcttccatgggacaaatcgccccagacagccagatcgacgccatgattaaagaggct
tccggaccaatcaacttcaccgtcttcctcacccttttcggagagagattgacaggaact
gacccagaggccaccattattggagcctttgccatgttcgacaagaaggactgcggaaag
atcaaggaggacgaccttatcaagatccttcaaaacaagagaggagagccattggacgag
gatgaggtcaaggctatgtacaagggaaagccaccaatcgagggaggagaggtcgactac
aaggccttcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACTGTTTGGCTTCGAATATTTTCT 458 143 229 Antisense
TATTTTCTCAAGTACATCTCTCTCA 91 663 246 Antisense
GTACATCTCTCTCAAAAGCAGTTAG 443 459 257 Antisense
AAGCAGTTAGTCTCAGTTCACTACT 668 149 272 Antisense
GTTCACTACTCAGTACAGTCCACAA 97 445 287 Antisense
AGTCCACAAAATGTCCAAGGCCGCC 8 61 303 Antisense
GGAATTCAAGGAGGCTTTCGGAATC 635 531 393 Antisense
TAAAGAGGCTTCCGGACCAATCAAC 539 637 519 Antisense
CAGAGGCCACCATTATTGGAGCCTT 128 207 593 Antisense
TTGGAGCCTTTGCCATGTTCGACAA 685 707 608 Antisense
GGAGAGGTCGACTACAAGGCCTTCG 357 517 754 Antisense
Sequence Source Affymetrix Proprietary Database