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[176623_at]

Description K04A8.4 /REP_DB=WormBase Gene ID /WP=CE11706 /GEN=twk-24 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 09      4grp_total > 22      6grp_cgc > 0      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 136
1 2 3 4 range
A 126.7
(2.1)
M 		45.1
(-3)
A 		78.4
(-0.9)
A 		140.6
(3)
M 		96
B 93
(3)
P 		49.8
(-2.2)
A 		43
(-3)
A 		61.1
(-0.8)
A 		50
C 90.8
(1.1)
P 		77.8
(0.5)
A 		4.8
(-3)
A 		130.7
(3)
A 		126
avr 103.5
(2.4) 		57.6
(-1.6) 		42.1
(-3) 		110.8
(3) 		69

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 47
1 2 3 4 5 6 range
A 52.3
(1.7)
A 		52.7
(1.8)
A 		50
(1.2)
A 		57.8
(3)
A 		38.8
(-1.4)
A 		32
(-3)
A 		26
B 72.8
(3)
A 		45
(-1.4)
A 		34.7
(-3)
A 		36
(-2.8)
A 		65
(1.8)
A 		71.5
(2.8)
A 		38
C 61.3
(0.4)
A 		51.4
(-1.1)
A 		42.6
(-2.4)
A 		78.9
(3)
A 		38.3
(-3)
P 		51
(-1.1)
A 		41
avr 62.1
(3) 		49.7
(-0.8) 		42.4
(-3) 		57.6
(1.6) 		47.4
(-1.5) 		51.5
(-0.2) 		20
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 44
1 2 3 4 range
A 46.1
(3)
M 		9.8
(-3)
A 		20.5
(-1.2)
A 		20.6
(-1.2)
M 		36
B 29.7
(3)
P 		12.3
(-2.3)
A 		10
(-3)
A 		13
(-2.1)
A 		20
C 30.1
(3)
P 		22.4
(1.4)
A 		1.7
(-3)
A 		22
(1.3)
A 		28
avr 35.3
(3) 		14.8
(-2) 		10.7
(-3) 		18.5
(-1.1) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 9.6
(-2)
A 		18.7
(3)
A 		10.9
(-1.3)
A 		14.7
(0.8)
A 		11.7
(-0.9)
A 		7.8
(-3)
A 		11
B 25.3
(2.5)
A 		15
(-0.8)
A 		8.4
(-3)
A 		9.5
(-2.6)
A 		26.4
(2.9)
A 		26.7
(3)
A 		18
C 22.7
(3)
A 		15.7
(-0.5)
A 		10.8
(-3)
A 		15.9
(-0.4)
A 		12.4
(-2.2)
P 		17.1
(0.2)
A 		12
avr 19.2
(3) 		16.5
(1.2) 		10
(-3) 		13.4
(-0.8) 		16.8
(1.4) 		17.2
(1.7) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176623_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12447
Sequence Type Exemplar sequence
Representative Public ID K04A8.4 NCBI
Target Description K04A8.4 /REP_DB=WormBase Gene ID /WP=CE11706 /GEN=twk-24 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072262(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072262 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-10) (twk-10) mRNA, complete cds. 11/11 None
SNAP00000001683 ENSEMBL cdna:SNAP chromosome:CEL140:V:6554942:6556680:1 11/11 None
GENEFINDER00000001696 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6554942:6556680:1 11/11 None
K04A8.4 ENSEMBL cdna:known chromosome:CEL140:V:6555063:6556577:1 gene:K04A8.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6555062-6556577(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol twk-10
Ensembl K04A8.4
Entrez Gene 192080 Entrez gene
SwissProt Q94253 EMBL-EBI
WormBase CE11706 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6813 potassium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5267 potassium channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC48050 Twik family of potassium channels protein 10 [Caenorhabditis elegans] ref|NP_504663.1| TWiK family of potassium channels family member (twk-10) [Caenorhabditis elegans] 1.0E-147
blast CAE71906 Hypothetical protein CBG18967 [Caenorhabditis briggsae] 1.0E-123
blast AAC48050 Twik family of potassium channels protein 10 [Caenorhabditis elegans] ref|NP_504663.1| TWiK family of potassium channels family member (twk-10) [Caenorhabditis elegans] 1.0E-112
blast CAE71906 Hypothetical protein CBG18967 [Caenorhabditis briggsae] 1.0E-98
blast AAC48050 Twik family of potassium channels protein 10 [Caenorhabditis elegans] ref|NP_504663.1| TWiK family of potassium channels family member (twk-10) [Caenorhabditis elegans] 5.0E-95
blast CAE71906 Hypothetical protein CBG18967 [Caenorhabditis briggsae] 3.0E-92
blast CAE57706 Hypothetical protein CBG00713 [Caenorhabditis briggsae] 2.0E-30
blast CAE57706 Hypothetical protein CBG00713 [Caenorhabditis briggsae] 2.0E-23
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504663.1 6 12-34,137-159,166-188,219-241,246-268,272-294
K04A8.4 6 12-34,137-159,166-188,219-241,246-268,272-294
SNAP00000001683 5 108-130,137-156,171-193,200-222,232-254
GENEFINDER00000001696 6 37-59,142-164,171-190,205-227,234-256,266-288
Sequence
Target Sequence 
>C. ELEGANS:176623_AT
atccggtagccgcgaacatgttcgattgccttttctattctcaatccaactacacaccac
tttggactaccgactcttctcttttattcactgcaactactatcattcctgttggatatg
gatacattgctccgttgacatcaactggaagaattgttctttgtatttacgcggcatttg
gaattccgctggctcttgtcatgatgtcagacgttggaaagttttttgcagacgcgtttg
tcaagttttttcacgaggttcgacgaatagttcaacgtttaaaaatgtctttaaaaattc
agaatataacagcgttcatggtagttcttctcatattgctagttgcatattctgtgattg
gaggaattgcgtactcaaaaatagttggagttccaatgattgaaggagtctatttcagta
caatcactatttttacaattggatttggagatataacaccgggtatccctgtatacgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCCGGTAGCCGCGAACATGTTCGA 84 37 362 Antisense
ACATGTTCGATTGCCTTTTCTATTC 558 105 377 Antisense
TTCTATTCTCAATCCAACTACACAC 272 691 394 Antisense
CTACACACCACTTTGGACTACCGAC 705 225 411 Antisense
TCTTCTCTTTTATTCACTGCAACTA 152 617 436 Antisense
TCACTGCAACTACTATCATTCCTGT 504 625 449 Antisense
CGGCATTTGGAATTCCGCTGGCTCT 195 243 533 Antisense
CCGCTGGCTCTTGTCATGATGTCAG 407 263 547 Antisense
ACAGCGTTCATGGTAGTTCTTCTCA 339 109 670 Antisense
GTTCTTCTCATATTGCTAGTTGCAT 389 443 685 Antisense
TAACACCGGGTATCCCTGTATACGT 222 637 815 Antisense
Sequence Source Affymetrix Proprietary Database