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[176621_s_at]

Description C50C3.9 /REP_DB=WormBase Gene ID /WP=CE24860 /GEN=unc-36 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 21      6grp_high1_10_10 > 13      4grp_highest_8_8 > 59      4grp_high_8_8 > 33      6grp_highest_nonorm > 51      6grp_total > 14      4grp_total > 47      6grp_highest_sub > De      6grp_cgc > 30      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 596
1 2 3 4 range
A 757.1
(3)
P 		391.2
(-1.4)
P 		261.4
(-3)
P 		303.6
(-2.5)
P 		496
B 837.6
(3)
P 		383.9
(-1.6)
P 		242
(-3)
P 		384.3
(-1.6)
P 		596
C 758.8
(3)
P 		353.5
(-3)
P 		391.9
(-2.4)
P 		431.3
(-1.8)
P 		405
avr 784.5
(3) 		376.2
(-2) 		298.4
(-3) 		373.1
(-2.1) 		486

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 503
1 2 3 4 5 6 range
A 656.3
(2.6)
P 		684.7
(3)
P 		581.2
(1.5)
P 		501.4
(0.4)
P 		349
(-1.8)
P 		262.6
(-3)
P 		422
B 637.6
(3)
P 		573.7
(1.7)
P 		488.8
(0)
P 		461.6
(-0.5)
P 		335.3
(-3)
P 		359.3
(-2.5)
P 		302
C 765.1
(3)
P 		689.3
(2.1)
P 		505
(-0.2)
P 		567.7
(0.6)
P 		310.7
(-2.6)
P 		279
(-3)
P 		486
avr 686.3
(3) 		649.2
(2.4) 		525
(0.5) 		510.2
(0.3) 		331.7
(-2.5) 		300.3
(-3) 		386
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 231
1 2 3 4 range
A 275.7
(3)
P 		84.9
(-1.9)
P 		68.3
(-2.4)
P 		44.4
(-3)
P 		231
B 267.1
(3)
P 		94.8
(-1.9)
P 		56.1
(-3)
P 		81.9
(-2.3)
P 		211
C 251.2
(3)
P 		101.6
(-2)
P 		136.6
(-0.9)
P 		72.7
(-3)
P 		179
avr 264.7
(3) 		93.8
(-2.2) 		87
(-2.4) 		66.3
(-3) 		198

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 220
1 2 3 4 5 6 range
A 121
(-1.1)
P 		243.5
(3)
P 		126.6
(-0.9)
P 		127.2
(-0.9)
P 		105.3
(-1.6)
P 		63.9
(-3)
P 		180
B 221.8
(3)
P 		191
(1.2)
P 		118.7
(-3)
P 		122.3
(-2.8)
P 		136.2
(-2)
P 		134
(-2.1)
P 		103
C 283.6
(3)
P 		210.3
(0.7)
P 		128.5
(-1.9)
P 		114.2
(-2.3)
P 		100.5
(-2.8)
P 		93.4
(-3)
P 		190
avr 208.8
(2.7) 		214.9
(3) 		124.6
(-1.6) 		121.2
(-1.8) 		114
(-2.1) 		97.1
(-3) 		118
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176621_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7688
Sequence Type Exemplar sequence
Representative Public ID C50C3.9 NCBI
Target Description C50C3.9 /REP_DB=WormBase Gene ID /WP=CE24860 /GEN=unc-36 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066388(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066388 NCBI Caenorhabditis elegans UNCoordinated family member (unc-36) (unc-36) mRNA, complete cds. 11/11 None
GENEFINDER00000020244 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8193958:8201022:-1 11/11 None
SNAP00000020238 ENSEMBL cdna:SNAP chromosome:CEL140:III:8194510:8201022:-1 9/11 None
C50C3.9 ENSEMBL cdna:known chromosome:CEL140:III:8193772:8201123:-1 gene:C50C3.9 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000020237 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8193953-8201018(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol unc-36
Ensembl C50C3.9
Entrez Gene 176155 Entrez gene
SwissProt P34374 EMBL-EBI
WormBase CE32168 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1595419_S_AT similar to calcium channel, voltage-dependent, alpha 2/delta 3 subunit cfa
CANINE:1586272_AT similar to calcium channel, voltage-dependent, alpha 2/delta 3 subunit cfa
CANINE_2:CFA.11051.1.A1_AT similar to calcium channel, voltage-dependent, alpha 2/delta 3 subunit cfa
CANINE_2:CFA.1728.1.A1_S_AT similar to calcium channel, voltage-dependent, alpha 2/delta 3 subunit cfa
CANINE_2:CFAAFFX.13064.1.S1_AT similar to calcium channel, voltage-dependent, alpha 2/delta 3 subunit cfa
DROSOPHILA_2:1627533_AT dm
DROSGENOME1:141545_AT dm
CHICKEN:GGA.19540.1.S1_AT similar to calcium channel, voltage dependent, alpha2/delta subunit 3 gga
CHICKEN:GGAAFFX.3394.1.S1_AT similar to calcium channel, voltage dependent, alpha2/delta subunit 3 gga
CHICKEN:GGAAFFX.3397.1.S1_AT similar to calcium channel, voltage dependent, alpha2/delta subunit 3 gga
HG-U133_PLUS_2:219714_S_AT calcium channel, voltage-dependent, alpha 2/delta 3 subunit hs
HG-U133A:219714_S_AT calcium channel, voltage-dependent, alpha 2/delta 3 subunit hs
HG-FOCUS:219714_S_AT calcium channel, voltage-dependent, alpha 2/delta 3 subunit hs
HG-U133A_2:219714_S_AT calcium channel, voltage-dependent, alpha 2/delta 3 subunit hs
HU35KSUBA:RC_AA459684_AT calcium channel, voltage-dependent, alpha 2/delta 3 subunit hs
U133_X3P:G8923764_3P_A_AT calcium channel, voltage-dependent, alpha 2/delta 3 subunit hs
HG-U95C:63591_AT calcium channel, voltage-dependent, alpha 2/delta 3 subunit hs
HG-U95D:74322_S_AT calcium channel, voltage-dependent, alpha 2/delta 3 subunit hs
HG-U95D:74324_R_AT calcium channel, voltage-dependent, alpha 2/delta 3 subunit hs
HG-U95D:74326_AT calcium channel, voltage-dependent, alpha 2/delta 3 subunit hs
HG-U95E:81701_AT calcium channel, voltage-dependent, alpha 2/delta 3 subunit hs
HG-U95C:66796_AT Calcium channel, voltage-dependent, alpha 2/delta 3 subunit hs
HG-U95D:87210_AT Calcium channel, voltage-dependent, alpha 2/delta 3 subunit hs
HG-U95D:88507_AT Calcium channel, voltage-dependent, alpha 2/delta 3 subunit hs
HU35KSUBB:RC_H09289_AT Calcium channel, voltage-dependent, alpha 2/delta 3 subunit hs
MG-U74AV2:98300_AT calcium channel, voltage-dependent, alpha2/delta subunit 3 mm
MOE430A:1419225_AT calcium channel, voltage-dependent, alpha2/delta subunit 3 mm
MOUSE430A_2:1419225_AT calcium channel, voltage-dependent, alpha2/delta subunit 3 mm
MOUSE430_2:1419225_AT calcium channel, voltage-dependent, alpha2/delta subunit 3 mm
RAE230A:1390358_AT calcium channel, voltage-dependent, alpha 2/delta 3 subunit rn
RAT230_2:1390358_AT calcium channel, voltage-dependent, alpha 2/delta 3 subunit rn
RAE230B:1397151_AT Calcium channel, voltage-dependent, alpha 2/delta 3 subunit rn
RAT230_2:1397151_AT Calcium channel, voltage-dependent, alpha 2/delta 3 subunit rn
RAE230B:1398190_AT Calcium channel, voltage-dependent, alpha 2/delta 3 subunit rn
RAT230_2:1398190_AT Calcium channel, voltage-dependent, alpha 2/delta 3 subunit rn
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA27971 Uncoordinated protein 36 [Caenorhabditis elegans] ref|NP_498789.2| UNCoordinated family member (unc-36) [Caenorhabditis elegans] sp|P34374|UNC36_CAEEL Voltage-dependent calcium channel unc-36 precursor (Uncoordinated protein 36) 0.0
blast CAE70192 Hypothetical protein CBG16666 [Caenorhabditis briggsae] 0.0
blast S44618 C50C3.10 protein - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002035 EMBL-EBI von Willebrand factor, type A 1.1E-21
Pfam IPR004010 EMBL-EBI Cache domain 3.6E-26
Pfam IPR002035 EMBL-EBI von Willebrand factor, type A 8.4E-25
Pfam IPR004010 EMBL-EBI Cache domain 3.6E-26
Sequence
Target Sequence 
>C. ELEGANS:176621_S_AT
aggttgtcactacatctggaaacagattaaggcctttctatccgattttccgattcttga
tgcaaactttcaactttatggttcgattagcaagtcagatatcaggcggatttttaatat
ggcttccaaatattcaattcacggaagcctacaccgcgtcttttcatgagggaacagatg
tttatccgtgtccaaaacaatcgtcattttatttttcgaataaagacggaaagaatcggc
ctggaacgacacatcttgttaatggtaatcgttctgagaggccgtgcaaaatgaatgcta
agtgttctgtcaaaatggaagcctcatttgttgatggaacaaatcttgtaatggtatgga
ttacacaggataaagccagtgagaattgttacgatgaatcagaatgttcaatggagattt
caaatcaagtaccattcgggtttgaagaggttaaaaatgaagaaacatgcgaagaaaatg
aaaaaagaaaatctaaagcgaatgatgtttgttattcaattgatgacgatgattctgaga
acgagcgccgtccttgctcaacgagccccacaatcgtgtcaattttcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGGTTGTCACTACATCTGGAAACAG 378 57 3002 Antisense
GATTAAGGCCTTTCTATCCGATTTT 61 431 3026 Antisense
TCCGATTTTCCGATTCTTGATGCAA 86 595 3042 Antisense
CAATTCACGGAAGCCTACACCGCGT 148 177 3136 Antisense
TACACCGCGTCTTTTCATGAGGGAA 2 643 3151 Antisense
GGGAACAGATGTTTATCCGTGTCCA 436 493 3171 Antisense
ATCCGTGTCCAAAACAATCGTCATT 53 39 3185 Antisense
GAATCGGCCTGGAACGACACATCTT 463 329 3234 Antisense
GGTAATCGTTCTGAGAGGCCGTGCA 608 503 3265 Antisense
TGATTCTGAGAACGAGCGCCGTCCT 660 563 3531 Antisense
AGCCCCACAATCGTGTCAATTTTCC 261 83 3565 Antisense
Sequence Source Affymetrix Proprietary Database