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[176620_at]

Description K12H4.1 /REP_DB=WormBase Gene ID /WP=CE00266 /GEN=ceh-26 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 16      4grp_total > 47      6grp_cgc > 11      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 280
1 2 3 4 range
A 391.1
(3)
P 		159.5
(-2.6)
A 		140.9
(-3)
A 		225.1
(-1)
P 		250
B 286.8
(3)
P 		197.9
(-0)
A 		198.9
(-0)
M 		111.3
(-3)
P 		176
C 293.3
(3)
P 		199
(-1.5)
A 		167
(-3)
A 		220.7
(-0.4)
P 		126
avr 323.7
(3) 		185.5
(-2.4) 		168.9
(-3) 		185.7
(-2.3) 		155

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 331
1 2 3 4 5 6 range
A 222
(3)
P 		219
(2.9)
P 		99.4
(-3)
P 		122.6
(-1.9)
A 		117.9
(-2.1)
A 		129.4
(-1.5)
A 		123
B 277
(3)
P 		167.2
(-0.6)
P 		108.4
(-2.5)
A 		104.5
(-2.6)
A 		92.3
(-3)
A 		100.6
(-2.7)
P 		185
C 423.6
(3)
P 		164.4
(-1.7)
P 		190
(-1.3)
A 		131.8
(-2.3)
A 		96
(-3)
P 		115.6
(-2.6)
P 		328
avr 307.5
(3) 		183.5
(-0.6) 		132.6
(-2.1) 		119.6
(-2.5) 		102.1
(-3) 		115.2
(-2.6) 		205
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 119
1 2 3 4 range
A 142.4
(3)
P 		34.6
(-2.9)
A 		36.8
(-2.8)
A 		32.9
(-3)
P 		110
B 91.5
(3)
P 		48.9
(-0.8)
A 		46.1
(-1)
M 		23.7
(-3)
P 		68
C 97.1
(3)
P 		57.2
(-1)
A 		58.2
(-0.9)
A 		37.2
(-3)
P 		60
avr 110.3
(3) 		46.9
(-1.8) 		47
(-1.8) 		31.3
(-3) 		79

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 135
1 2 3 4 5 6 range
A 40.9
(-1)
P 		77.9
(3)
P 		21.7
(-3)
P 		31.1
(-2)
A 		35.6
(-1.5)
A 		31.5
(-2)
A 		56
B 96.4
(3)
P 		55.7
(-0.5)
P 		26.3
(-3)
A 		27.7
(-2.9)
A 		37.5
(-2)
A 		37.5
(-2)
P 		70
C 157
(3)
P 		50.2
(-1.9)
P 		48.4
(-2)
A 		26.5
(-3)
A 		31.1
(-2.8)
P 		38.7
(-2.4)
P 		131
avr 98.1
(3) 		61.3
(-0.2) 		32.1
(-2.7) 		28.4
(-3) 		34.7
(-2.5) 		35.9
(-2.4) 		70
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176620_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6219
Sequence Type Exemplar sequence
Representative Public ID K12H4.1 NCBI
Target Description K12H4.1 /REP_DB=WormBase Gene ID /WP=CE00266 /GEN=ceh-26 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066359(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066359 NCBI Caenorhabditis elegans C.Elegans Homeobox family member (ceh-26) (ceh-26) mRNA, complete cds. 11/11 None
GENEFINDER00000000474 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8067261:8070946:1 11/11 None
K12H4.1 ENSEMBL cdna:known chromosome:CEL140:III:8067216:8071343:1 gene:K12H4.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000000457 6/11 Cross Hyb Matching Probes None
SNAP00000000459 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8067258-8070944(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ceh-26
Ensembl K12H4.1
Entrez Gene 176137 Entrez gene
SwissProt P34522 EMBL-EBI
WormBase CE00266 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_498760 C.Elegans Homeobox family member (ceh-26) [Caenorhabditis elegans] gb|AAA28100.1| C.elegans homeobox protein 26 [Caenorhabditis elegans] sp|P34522|HM26_CAEEL Homeobox protein ceh-26 0.0
blast CAE75068 Hypothetical protein CBG22984 [Caenorhabditis briggsae] 1.0E-130
blast EAA05345 ENSANGP00000010936 [Anopheles gambiae str. PEST] ref|XP_309606.2| ENSANGP00000010936 [Anopheles gambiae str. PEST] 4.0E-61
Protein Domains
Database ID Description E-Value
scop a.4.1.Homeodomain All alpha proteins; DNA/RNA-binding 3-helical bundle; Homeodomain-like; Homeodomain 0.0
Pfam IPR007738 EMBL-EBI Homeobox prospero-like 4.9E-106
Sequence
Target Sequence 
>C. ELEGANS:176620_AT
gattctttccagcaccaactatggttggacactacggtggaatgaactttggagatcgag
aggactcaccaacaaattccgatgagctcagtgaatgtggatatgaaggtggtggctcaa
gttcaatgttaacaccaatgcacttgagaaaggcaaagctgatgtttttctacacacgat
atccaaattcaaatctgctgaaatcgtatttccctgatatccgattcaataagaataaca
ctgcacaattggtgaaatggttcagtaacttcagagagttctactacaatcagatggaga
agttcgcgagacaagctctcgctgagggaattactgatagaaatgatatttttgtgtcaa
aagacagtgaacttttcaaagttttgaatacacattataacagaaataatcatattaagg
caccagatcgtcttgtatttgtggttcaagagactcttcgtgagtttcatgatgcaatca
aacagggaaaagatattgaaccatcatggaagaaaacaatctataaggttatcaatcgat
tggaagatcagattcccgacttcttcaagga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTCTTTCCAGCACCAACTATGGT 627 427 1172 Antisense
AACTATGGTTGGACACTACGGTGGA 490 137 1188 Antisense
GGAGATCGAGAGGACTCACCAACAA 89 519 1222 Antisense
CACCAACAAATTCCGATGAGCTCAG 87 203 1238 Antisense
GATGTTTTTCTACACACGATATCCA 321 409 1332 Antisense
GTATTTCCCTGATATCCGATTCAAT 86 453 1377 Antisense
TGGAGAAGTTCGCGAGACAAGCTCT 462 549 1466 Antisense
GACAAGCTCTCGCTGAGGGAATTAC 232 365 1481 Antisense
AGGCACCAGATCGTCTTGTATTTGT 539 53 1589 Antisense
ACTCTTCGTGAGTTTCATGATGCAA 510 99 1624 Antisense
ATCAGATTCCCGACTTCTTCAAGGA 574 27 1718 Antisense
Sequence Source Affymetrix Proprietary Database