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[176619_s_at]

Description E01A2.3 /REP_DB=WormBase Gene ID /WP=CE24870 /GEN=lin-44 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 23      6grp_high1_10_10 > 21      4grp_highest_8_8 > 15      4grp_high_8_8 > 13      6grp_highest_nonorm > 90      6grp_total > 32      4grp_total > 10      6grp_highest_sub > 01_02_03      6grp_cgc > 71      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 121
1 2 3 4 range
A 100.4
(-2.9)
P 		119.2
(-1.9)
P 		97.7
(-3)
P 		218.4
(3)
P 		121
B 117
(-3)
P 		132.5
(1.8)
P 		136.4
(3)
P 		130.4
(1.1)
P 		19
C 123.6
(-1.7)
P 		112.2
(-2.4)
P 		103.9
(-3)
P 		193
(3)
P 		89
avr 113.7
(-2.9) 		121.3
(-2.2) 		112.7
(-3) 		180.6
(3) 		68

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 110
1 2 3 4 5 6 range
A 118.6
(2.9)
P 		83.1
(-0)
P 		120
(3)
P 		80.5
(-0.2)
P 		73.3
(-0.8)
P 		46.8
(-3)
P 		73
B 120.5
(0.8)
P 		124
(1)
P 		156.8
(3)
P 		59.2
(-3)
P 		77.1
(-1.9)
P 		70.3
(-2.3)
P 		98
C 96
(-0.1)
P 		138.1
(2.8)
P 		84.2
(-0.9)
P 		141.6
(3)
P 		53.9
(-3)
P 		67.3
(-2.1)
P 		88
avr 111.7
(2.1) 		115.1
(2.5) 		120.3
(3) 		93.8
(0.3) 		68.1
(-2.3) 		61.5
(-3) 		59
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 36.6
(3)
P 		25.9
(-2.8)
P 		25.5
(-3)
P 		32
(0.5)
P 		11
B 37.3
(3)
P 		32.7
(0.1)
P 		31.6
(-0.6)
P 		27.8
(-3)
P 		10
C 40.9
(3)
P 		32.3
(-3)
P 		36.2
(-0.3)
P 		32.5
(-2.9)
P 		9
avr 38.3
(3) 		30.3
(-3) 		31.1
(-2.4) 		30.8
(-2.6) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 31
1 2 3 4 5 6 range
A 21.9
(0.5)
P 		29.6
(3)
P 		26.1
(1.8)
P 		20.4
(-0)
P 		22.1
(0.5)
P 		11.4
(-3)
P 		18
B 41.9
(3)
P 		41.3
(2.9)
P 		38.1
(2.1)
P 		15.7
(-3)
P 		31.3
(0.6)
P 		26.2
(-0.6)
P 		26
C 35.6
(1.4)
P 		42.1
(3)
P 		21.4
(-2)
P 		28.5
(-0.3)
P 		17.5
(-3)
P 		22.5
(-1.8)
P 		25
avr 33.1
(1.4) 		37.7
(3) 		28.5
(-0.1) 		21.5
(-2.5) 		23.6
(-1.8) 		20
(-3) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176619_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1339
Sequence Type Exemplar sequence
Representative Public ID E01A2.3 NCBI
Target Description E01A2.3 /REP_DB=WormBase Gene ID /WP=CE24870 /GEN=lin-44 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001025911(9), NM_001025910(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001025911 NCBI Caenorhabditis elegans abnormal cell LINeage family member (lin-44) (lin-44) mRNA, complete cds. 9/11 None
NM_001025910 NCBI Caenorhabditis elegans abnormal cell LINeage family member (lin-44) (lin-44) mRNA, complete cds. 11/11 None
SNAP00000008530 ENSEMBL cdna:SNAP chromosome:CEL140:I:4126179:4129102:1 11/11 None
GENEFINDER00000008596 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:4126179:4129235:1 11/11 None
E01A2.3b ENSEMBL cdna:known chromosome:CEL140:I:4126057:4129301:1 gene:E01A2.3 9/11 None
E01A2.3a ENSEMBL cdna:known chromosome:CEL140:I:4126147:4129311:1 gene:E01A2.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4126178-4129102(+) 98.95 100.0
Public Domain and Genome References
Ensembl E01A2.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK85460 Abnormal cell lineage protein 44, isoform a [Caenorhabditis elegans] ref|NP_001021081.1| abnormal cell LINeage family member (lin-44) [Caenorhabditis elegans] gb|AAA64847.1| Wnt protein gb|AAA64846.1| Wnt protein prf||2122239A lin-44 gene 0.0
blast CAE66720 Hypothetical protein CBG12066 [Caenorhabditis briggsae] 0.0
blast AAT68899 Abnormal cell lineage protein 44, isoform b [Caenorhabditis elegans] ref|NP_001021082.1| abnormal cell LINeage family member (lin-44) [Caenorhabditis elegans] 1.0E-100
Protein Domains
Database ID Description E-Value
Pfam IPR005817 EMBL-EBI Wnt superfamily 5.1E-17
Pfam IPR005817 EMBL-EBI Wnt superfamily 2.5E-10
Pfam IPR005817 EMBL-EBI Wnt superfamily 2.3E-9
Pfam IPR005817 EMBL-EBI Wnt superfamily 3.3E-22
Pfam IPR005817 EMBL-EBI Wnt superfamily 2.5E-10
Sequence
Target Sequence 
>C. ELEGANS:176619_S_AT
gttcggcggatgtactcatggtgttcaacacggaataacggctagtcgaaagctgctcac
aaaggtcggagcggtgaatactttgttaaggaaggtggagaagcacaatttgaaggcagg
gagattggccatcaaaaagaccctaatctcttcctgcaaatgccacggagtatccggttc
ttgtcaacagaaaacttgctggaaacgaactgcaacccttgagcacattaccgattattt
agtggaaaagtatgcacgagccaagctctatacggatgattcggtggtaaagacgacgga
tttgatatatttagaagcgtctccggatgtttgcaaggcaaaatcagttgccggcagagt
gtgtgcatggaggaatgagacgcatacccaaggtgattgtgatcggctgtgttgcgggaa
tggattcagcattcgtcacgaagtggtccgtgtgaagtgtgactgtgaattcgtgtggtg
ttgcaatctggtctgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTCGGCGGATGTACTCATGGTGTT 201 443 495 Antisense
AACGGCTAGTCGAAAGCTGCTCACA 195 143 531 Antisense
CTAATCTCTTCCTGCAAATGCCACG 138 227 637 Antisense
TGCCACGGAGTATCCGGTTCTTGTC 238 569 655 Antisense
GAACTGCAACCCTTGAGCACATTAC 115 349 701 Antisense
GAGCCAAGCTCTATACGGATGATTC 294 385 752 Antisense
TAGAAGCGTCTCCGGATGTTTGCAA 56 651 806 Antisense
GTTGCCGGCAGAGTGTGTGCATGGA 159 441 841 Antisense
GATTGTGATCGGCTGTGTTGCGGGA 70 435 889 Antisense
ATGGATTCAGCATTCGTCACGAAGT 168 51 914 Antisense
CGTGTGGTGTTGCAATCTGGTCTGT 221 245 966 Antisense
Sequence Source Affymetrix Proprietary Database