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[176617_s_at]

Description K08D12.1 /REP_DB=WormBase Gene ID /WP=CE25941 /GEN=pbs-1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 27      6grp_high1_10_10 > 62      4grp_highest_8_8 > 02      4grp_high_8_8 > 46      6grp_highest_nonorm > 57      6grp_total > 53      4grp_total > 49      6grp_highest_sub > 03_04      6grp_cgc > 90      6grp_high1_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2849
1 2 3 4 range
A 2414.8
(-3)
P 		4552.6
(2.5)
P 		4726.4
(3)
P 		2916.4
(-1.7)
P 		2312
B 2313.5
(-3)
P 		4102.7
(1.3)
P 		4827.3
(3)
P 		2601.2
(-2.3)
P 		2514
C 1978.7
(-3)
P 		4270.7
(2.9)
P 		4305.1
(3)
P 		2416.6
(-1.9)
P 		2326
avr 2235.7
(-3) 		4308.7
(2.2) 		4619.6
(3) 		2644.7
(-2) 		2384

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1088
1 2 3 4 5 6 range
A 2525.2
(-0.7)
P 		2477
(-1.4)
P 		2768.1
(3)
P 		2727.4
(2.4)
P 		2533.7
(-0.6)
P 		2374.6
(-3)
P 		394
B 2794.7
(0.2)
P 		2316.3
(-3)
P 		3202.6
(3)
P 		2866.5
(0.7)
P 		2915.1
(1.1)
P 		2933.1
(1.2)
P 		886
C 2385.8
(-0.9)
P 		2473.7
(-0.3)
P 		2765.5
(2)
P 		2232.8
(-2.1)
P 		2899.8
(3)
P 		2114.4
(-3)
P 		785
avr 2568.6
(-1.2) 		2422.3
(-3) 		2912.1
(3) 		2608.9
(-0.7) 		2782.9
(1.4) 		2474
(-2.4) 		490
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1093
1 2 3 4 range
A 879.2
(0.4)
P 		988.2
(1.2)
P 		1235.6
(3)
P 		426.9
(-3)
P 		809
B 737.8
(-1)
P 		1013.3
(1.9)
P 		1118.6
(3)
P 		554.3
(-3)
P 		564
C 655
(-1.6)
P 		1227.8
(1.5)
P 		1500.5
(3)
P 		407.5
(-3)
P 		1093
avr 757.3
(-0.9) 		1076.4
(1.5) 		1284.9
(3) 		462.9
(-3) 		822

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 735
1 2 3 4 5 6 range
A 465.5
(-3)
P 		881
(3)
P 		602.8
(-1)
P 		691.7
(0.3)
P 		764.6
(1.3)
P 		578.2
(-1.4)
P 		416
B 972.4
(0)
P 		771
(-2.8)
P 		777.7
(-2.7)
P 		759.2
(-3)
P 		1184.4
(3)
P 		1093.7
(1.7)
P 		425
C 884.3
(2.3)
P 		754.8
(0.7)
P 		703.9
(0.1)
P 		449
(-3)
P 		938.3
(3)
P 		707.8
(0.2)
P 		489
avr 774.1
(-0.4) 		802.3
(0.1) 		694.8
(-1.9) 		633.3
(-3) 		962.4
(3) 		793.2
(-0.1) 		329
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176617_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10378
Sequence Type Exemplar sequence
Representative Public ID K08D12.1 NCBI
Target Description K08D12.1 /REP_DB=WormBase Gene ID /WP=CE25941 /GEN=pbs-1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067724(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067724 NCBI Caenorhabditis elegans Proteasome Beta Subunit family member (pbs-1) (pbs-1) mRNA, complete cds. 11/11 None
SNAP00000039170 ENSEMBL cdna:SNAP chromosome:CEL140:IV:1708902:1709740:-1 11/11 None
GENEFINDER00000039179 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:1708902:1709740:-1 11/11 None
K08D12.1 ENSEMBL cdna:known chromosome:CEL140:IV:1708818:1709743:-1 gene:K08D12.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:1708907-1709737(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol pbs-1
Ensembl K08D12.1
Entrez Gene 176978 Entrez gene
SwissProt Q966I8 EMBL-EBI
WormBase CE25941 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:253521_AT 20S proteasome beta subunit A (PBA1) (PRCD) at
CANINE_2:CFA.2649.1.S1_S_AT similar to Proteasome subunit beta type 6 precursor (Proteasome delta chain) (Macropain delta chain) (Multicatalytic endopeptidase complex delta chain) (Proteasome subunit Y) cfa
DROSOPHILA_2:1641616_AT dm
DROSGENOME1:153183_AT dm
HG-U95AV2:941_AT proteasome (prosome, macropain) subunit, beta type, 6 hs
HC-G110:941_AT proteasome (prosome, macropain) subunit, beta type, 6 hs
HG-U133A:208827_AT proteasome (prosome, macropain) subunit, beta type, 6 hs
HG-U133_PLUS_2:208827_AT proteasome (prosome, macropain) subunit, beta type, 6 hs
HG-U133A_2:208827_AT proteasome (prosome, macropain) subunit, beta type, 6 hs
HUGENEFL:D29012_AT proteasome (prosome, macropain) subunit, beta type, 6 hs
U133_X3P:G12654058_3P_AT proteasome (prosome, macropain) subunit, beta type, 6 hs
MOE430A:1448822_AT proteasome (prosome, macropain) subunit, beta type 6 mm
MOUSE430_2:1448822_AT proteasome (prosome, macropain) subunit, beta type 6 mm
MOUSE430A_2:1448822_AT proteasome (prosome, macropain) subunit, beta type 6 mm
MU11KSUBA:U13393_S_AT proteasome (prosome, macropain) subunit, beta type 6 mm
MG-U74AV2:101992_AT proteasome (prosome, macropain) subunit, beta type 6 mm
MU19KSUBA:TC14279_I_AT Proteasome (prosome, macropain) subunit, beta type 6, mRNA (cDNA clone MGC:14026 IMAGE:4038758) mm
MU19KSUBA:TC14280_AT Proteasome (prosome, macropain) subunit, beta type 6, mRNA (cDNA clone MGC:14026 IMAGE:4038758) mm
MU19KSUBB:TC33505_AT Proteasome (prosome, macropain) subunit, beta type 6, mRNA (cDNA clone MGC:14026 IMAGE:4038758) mm
RG-U34A:D10754_G_AT proteasome (prosome, macropain) subunit, beta type 6 rn
RG-U34A:D10754_AT proteasome (prosome, macropain) subunit, beta type 6 rn
RAE230A:1398777_AT proteasome (prosome, macropain) subunit, beta type 6 rn
RAT230_2:1398777_AT proteasome (prosome, macropain) subunit, beta type 6 rn
YEAST_2:1779845_AT 20S proteasome beta-type subunit, responsible for cleavage after acidic residues in peptides Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6511 ubiquitin-dependent protein catabolism inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5839 proteasome core complex (sensu Eukaryota) inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4175 endopeptidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK84540 Proteasome beta subunit protein 1 [Caenorhabditis elegans] ref|NP_500125.1| Proteasome Beta Subunit family member (pbs-1) [Caenorhabditis elegans] 1.0E-133
blast CAE67980 Hypothetical protein CBG13586 [Caenorhabditis briggsae] 1.0E-132
blast CAE67980 Hypothetical protein CBG13586 [Caenorhabditis briggsae] 1.0E-130
blast AAF58077 CG8392-PA [Drosophila melanogaster] ref|NP_652031.2| CG8392-PA [Drosophila melanogaster] 3.0E-56
Protein Domains
Database ID Description E-Value
Pfam IPR001353 EMBL-EBI Peptidase T1, 20S proteasome 1.1E-64
Pfam IPR001353 EMBL-EBI Peptidase T1, 20S proteasome 1.1E-64
Sequence
Target Sequence 
>C. ELEGANS:176617_S_AT
cgtcggaaccgactcgagaaccagcgccggctcgttcatcacctcccgtgccaccaacaa
aatcaccccgatcaccgataatatggttgtgtgccgttctggatctgcagcggacactca
agcgatcgccgatattgccaagtaccatattgatgtttataccatgacagagaacaagcc
agtgacaatctaccgctcgagccagattttccgccaattcctctacaactaccgcgagca
gctctccgcctcagtgctcgtcgccggctgggatgaggagctcggcggccaggtctacgc
aatcccaatcggaggattcgtgtcccgccagcgatcgacagcttccggctccggttccac
attcgtgcagggcttcctcgacagtcaatggagaccaaacttgacgctcgaggagtgcaa
ggcgatcgtgaagcaagccgtcggactcgccaccttccgtgacggatcctccggcggagt
tgtccgtctcgccgtgatcaacaaagccggctcacagttcgagctgttccgtcccgacaa
accaggattcccattcgtgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGTCGGAACCGACTCGAGAACCAGC 625 243 120 Antisense
ATCACCCCGATCACCGATAATATGG 638 25 181 Antisense
TATGGTTGTGTGCCGTTCTGGATCT 309 655 201 Antisense
TGCAGCGGACACTCAAGCGATCGCC 110 579 225 Antisense
CAAGCCAGTGACAATCTACCGCTCG 29 187 294 Antisense
GGGCTTCCTCGACAGTCAATGGAGA 84 491 489 Antisense
GGAGACCAAACTTGACGCTCGAGGA 409 519 509 Antisense
GATCGTGAAGCAAGCCGTCGGACTC 660 417 543 Antisense
CGGATCCTCCGGCGGAGTTGTCCGT 351 1 582 Antisense
AAGCCGGCTCACAGTTCGAGCTGTT 452 149 623 Antisense
GACAAACCAGGATTCCCATTCGTGC 706 365 655 Antisense
Sequence Source Affymetrix Proprietary Database