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[176614_at] 7TM chemoreceptor, srg family

Description T12A2.12 /REP_DB=WormBase Gene ID /WP=CE25994 /GEN=srg-4 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 23      4grp_total > 28      6grp_cgc > 30      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 48
1 2 3 4 range
A 26.5
(-0)
A 		44.1
(2.3)
A 		4.1
(-3)
A 		49.2
(3)
P 		45
B 37.1
(3)
P 		1.4
(-3)
A 		25.8
(1.1)
A 		24.9
(0.9)
A 		36
C 12
(-3)
A 		38.3
(3)
A 		15.2
(-2.3)
A 		27
(0.4)
A 		26
avr 25.2
(0.3) 		27.9
(1.1) 		15
(-3) 		33.7
(3) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 54
1 2 3 4 5 6 range
A 33.9
(3)
A 		22.4
(-1.4)
A 		30.5
(1.7)
A 		31.1
(1.9)
A 		18.1
(-3)
A 		21.7
(-1.6)
A 		16
B 38.8
(3)
A 		9.3
(-2)
A 		23
(0.3)
A 		17.3
(-0.6)
A 		14.4
(-1.1)
A 		3.2
(-3)
A 		36
C 25.4
(-1.6)
A 		57.6
(3)
A 		35.7
(-0.1)
A 		29
(-1)
A 		15.2
(-3)
A 		18.6
(-2.5)
A 		42
avr 32.7
(3) 		29.8
(2) 		29.7
(2) 		25.8
(0.7) 		15.9
(-2.5) 		14.5
(-3) 		18
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 9.6
(3)
A 		9.6
(3)
A 		1.1
(-3)
A 		7.2
(1.3)
P 		9
B 11.8
(3)
P 		0.3
(-3)
A 		6
(-0)
A 		5.3
(-0.4)
A 		12
C 4
(-3)
A 		11
(3)
A 		5.3
(-1.9)
A 		4.6
(-2.5)
A 		7
avr 8.5
(3) 		7
(1) 		4.1
(-3) 		5.7
(-0.8) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 6.2
(-1)
A 		8
(3)
A 		6.6
(-0.1)
A 		7.9
(2.8)
A 		5.5
(-2.6)
A 		5.3
(-3)
A 		3
B 13.5
(3)
A 		3.1
(-2.1)
A 		5.6
(-0.9)
A 		4.6
(-1.3)
A 		5.9
(-0.7)
A 		1.2
(-3)
A 		12
C 9.4
(-0.9)
A 		17.6
(3)
A 		9.1
(-1)
A 		5.8
(-2.6)
A 		4.9
(-3)
A 		6.2
(-2.4)
A 		13
avr 9.7
(3) 		9.6
(2.9) 		7.1
(0.2) 		6.1
(-0.9) 		5.4
(-1.7) 		4.2
(-3) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176614_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6724
Sequence Type Exemplar sequence
Representative Public ID T12A2.12 NCBI
Target Description T12A2.12 /REP_DB=WormBase Gene ID /WP=CE25994 /GEN=srg-4 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065967(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065967 NCBI Caenorhabditis elegans Serpentine Receptor, class G (gamma) family member (srg-4) (srg-4) mRNA, complete cds. 11/11 None
T12A2.12 ENSEMBL cdna:known chromosome:CEL140:III:6260155:6262024:-1 gene:T12A2.12 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000044244 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6260927-6262023(-) 100.0 100.0
Public Domain and Genome References
Gene Title 7TM chemoreceptor, srg family
Gene Symbol srg-4
Ensembl T12A2.12
Entrez Gene 191830 Entrez gene
SwissProt P54126 EMBL-EBI
WormBase CE33181 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7165 signal transduction inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4888 transmembrane receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK84568 Serpentine receptor, class g (gamma) protein 4 [Caenorhabditis elegans] ref|NP_498368.2| Serpentine Receptor, class G (gamma) family member (srg-4) [Caenorhabditis elegans] sp|P54126|SRG4_CAEEL Serpentine receptor class gamma-4 (Protein srg-4) 1.0E-171
blast 2123261AD chemosensory receptor 1.0E-167
blast 2123261AE chemosensory receptor 3.0E-64
Protein Domains
Database ID Description E-Value
Pfam IPR000609 EMBL-EBI Integral membrane protein Srg, nematode type 1.6E-93
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498368.2 6 21-43,99-121,142-160,188-206,227-249,259-281
T12A2.12 6 21-43,99-121,142-160,188-206,227-249,259-281
Sequence
Target Sequence 
>C. ELEGANS:176614_AT
ttcacatatatttcccacagctttgtgaaggattctcagtgtttttggaaattcactgga
tgattccaaacattgtataccccttttatttgtacgcgttcactgcaaaatcagtgatcc
actcgtttttgtcgataaatagagcaagctgcgtgttgatgccgacaaaatacgcatata
tatggcgctctcatatgaagaaagttattgtttttatacttctctatccatttttgctac
tttggaatgtgattatatcggaaaaatatttggattttatatttggaggctttgcaagcc
tatccaaatttcaaataatctcttatgtattcacattctcggtaacacttgtcacaaata
gtataactttatcaaaaatggcaaaactcaaaaagcggcttctaatggcggaacggcatc
tctgcgttgccacagcatggatttcctccggatttgtgatttcactgattgctcaagcac
attttgcattttttcgagggg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCACATATATTTCCCACAGCTTTG 112 687 236 Antisense
CCCTTTTATTTGTACGCGTTCACTG 351 255 316 Antisense
TCAGTGATCCACTCGTTTTTGTCGA 474 623 346 Antisense
AGAGCAAGCTGCGTGTTGATGCCGA 322 69 376 Antisense
GTTTTTATACTTCTCTATCCATTTT 259 449 445 Antisense
GTATTCACATTCTCGGTAACACTTG 9 453 562 Antisense
TCTAATGGCGGAACGGCATCTCTGC 565 613 636 Antisense
ATCTCTGCGTTGCCACAGCATGGAT 115 33 653 Antisense
ATTTCCTCCGGATTTGTGATTTCAC 228 17 676 Antisense
GTGATTTCACTGATTGCTCAAGCAC 110 485 691 Antisense
GCACATTTTGCATTTTTTCGAGGGG 326 315 712 Antisense
Sequence Source Affymetrix Proprietary Database